cDNA Clone PHIP

• Synonyms: .
• Plasmid: pBabeD P Flag PHIP 5GLY aGFP16
• Parent Plasmid: pBabeD P Flag C-5GLY aGFP16
• Expressed: Flag PHIP 5GLY aGFP16
• Genbank: GeneID 55023
• Tag: FLAG
• Species: Human

Sequence of Insert

GGATCCATGTCTTGTGAGAGGAAAGGCCTCTCGGAGCTGCGATCGGAGCTCTACTTCCTCATCGCCCGGTTCCTGGAAGATGGACCCTGTCAGCAGGCGGCTCAGGTGCTGATCCGCGAGGTGGCCGAGAAGGAGCTGCTGCCCCGGCGCACCGACTGGACCGGGAAGGAGCATCCCAGGACCTACCAGAATCTGGTGAAGTATTACAGACACTTAGCACCTGATCACTTGCTGCAAATATGTCATCGACTAGGACCTCTTCTTGAACAAGAAATTCCTCAAAGTGTTCCTGGAGTACAAACTTTATTAGGAGCTGGAAGACAGTCTTTACTACGCACAAATAAAAGCTGCAAGCATGTTGTGTGGAAAGGATCTGCTCTGGCTGCGTTGCACTGTGGAAGACCACCTGAGTCACCAGTTAACTATGGTAGCCCACCCAGCATTGCGGATACTCTGTTTTCAAGGAAGCTGAATGGGAAATACAGACTTGAGCGACTTGTTCCAACTGCAGTGTATCAGCACATGAAAATGCATAAACGAATTCTTGGACACTTGTCATCTGTGTACTGTGTAACTTTTGATCGAACTGGCAGACGGATATTTACTGGTTCTGATGACTGTCTTGTGAAAATATGGGCAACAGATGATGGGAGGTTGTTAGCTACCTTAAGAGGACATGCTGCTGAAATATCAGACATGGCTGTAAACTATGAGAATACCATGATAGCAGCTGGAAGTTGTGATAAAATGATCCGAGTCTGGTGTCTTCGAACCTGTGCACCTTTGGCTGTTCTTCAGGGCCATAGTGCATCTATTACATCACTACAGTTCTCACCATTGTGCAGTGGCTCAAAGAGATATCTATCTTCTACTGGGGCAGATGGCACTATTTGTTTTTGGCTCTGGGATGCTGGAACCCTTAAAATAAACCCAAGACCTGCAAAATTTACAGAGCGCCCTCGGCCTGGAGTTCAAATGATCTGTTCTTCTTTTAGTGCTGGTGGAATGTTTCTGGCGACGGGAAGCACAGATCATATTATTCGGGTTTATTTTTTTGGATCAGGTCAGCCAGAGAAAATATCAGAATTGGAGTTTCATACTGACAAAGTTGACAGTATCCAGTTTTCCAACACTAGTAACAGGTTTGTAAGTGGCAGTCGTGATGGGACAGCACGTATTTGGCAATTTAAACGAAGAGAGTGGAAGAGCATTTTGTTGGATATGGCTACTCGTCCAGCAGGCCAAAACCTTCAAGGAATAGAAGATAAAATCACAAAAATGAAGGTTACTATGGTAGCTTGGGATCGACATGACAATACAGTTATAACTGCAGTTAATAACATGACTCTGAAAGTTTGGAATTCTTACACTGGTCAACTAATTCATGTCCTGATGGGTCATGAAGATGAGGTATTTGTTCTTGAACCACACCCGTTCGATCCTAGAGTTCTCTTTTCTGCTGGTCATGATGGAAACGTGATAGTGTGGGATCTGGCAAGAGGAGTCAAAATACGATCTTATTTCAATATGATTGAAGGCCAAGGACATGGCGCAGTATTTGACTGCAAATGCTCTCCTGATGGTCAGCATTTTGCATGCACAGACTCTCATGGACATCTTTTAATTTTTGGCTTTGGGTCCAGTAGCAAATATGACAAGATAGCAGATCAGATGTTCTTTCATAGTGATTATCGGCCACTTATTCGTGATGCCAACAATTTTGTATTAGATGAACAGACTCAGCAAGCACCTCATCTTATGCCTCCCCCTTTTTTGGTTGATGTTGATGGTAACCCTCATCCATCAAGATATCAAAGATTAGTTCCTGGCCGTGAAAATTGCAGGGAGGAGCAACTCATCCCTCAGATGGGAGTAACTTCCTCAGGACTGAATCAAGTTTTAAGTCAGCAAGCAAACCAGGAGATCAGCCCACTGGACAGCATGATTCAAAGACTACAACAGGAGCAAGACCTGAGACGTTCTGGTGAAGCAGGTATCAGTAATACCAGCCGTTTAAGTAGAGGCTCCATAAGTTCTACCTCAGAGGTTCATTCACCACCAAACGTAGGACTAAGACGTAGTGGACAAATTGAAGGTGTACGGCAAATGCACAGCAACGCACCAAGAAGTGAAATAGCCACAGAGCGGGATCTGGTAGCTTGGAGTCGAAGGGTGGTAGTACCCGAGCTATCAGCTGGTGTAGCCAGTAGGCAAGAAGAATGGAGAACTGCAAAGGGAGAAGAAGAAATAAAGACTTACAGGTCAGAAGAGAAAAGAAAACACTTAACTGTTCCAAAAGAGAATAAAATACCCACTGTCTCAAAGAATCATGCTCATGAGCATTTCCTGGATCTTGGAGAATCCAAAAAGCAACAGACAAATCAACACAATTATCGTACAAGATCTGCATTGGAAGAGACTCCTAGACCCTCAGAAGAGATAGAAAATGGCAGTAGTTCTTCAGATGAAGGCGAAGTAGTTGCTGTCAGTGGTGGAACATCCGAAGAAGAAGAGAGAGCATGGCACAGTGATGGCAGTTCTAGTGACTACTCCAGTGATTACTCTGACTGGACAGCAGATGCAGGAATTAATCTGCAGCCACCAAAGAAAGTTCCTAAGAATAAAACCAAGAAAGCAGAAAGCAGTTCAGATGAAGAAGAAGAATCTGAAAAACAGAAGCAAAAACAGATTAAAAAGGAAAAGAAAAAAGTAAATGAAGAAAAAGATGGACCAATATCACCAAAGAAAAAGAAGCCCAAAGAAAGAAAACAAAAGAGATTGGCTGTGGGAGAACTAACTGAAAATGGTTTGACATTAGAAGAATGGTTGCCATCAACGTGGATTACAGATACCATTCCCCGAAGATGTCCATTTGTGCCACAGATGGGTGATGAGGTTTATTATTTCCGACAAGGACATGAAGCCTATGTCGAAATGGCCCGGAAAAATAAAATATATAGTATCAATCCCAAAAAACAACCATGGCATAAAATGGAGCTACGGGAACAAGAACTTATGAAAATAGTTGGCATAAAGTATGAAGTGGGATTACCTACCCTTTGCTGCCTTAAACTTGCTTTTCTAGATCCTGATACTGGTAAACTGACTGGCGGATCATTTACCATGAAATACCATGATATGCCTGACGTTATAGATTTCCTAGTCTTGAGACAACAATTTGATGATGCAAAATACAGGCGATGGAATATAGGTGACCGCTTCAGGTCTGTCATAGATGATGCCTGGTGGTTTGGAACAATCGAAAGCCAGGAACCTCTTCAACCTGAGTACCCTGATAGTCTGTTTCAATGCTACAATGTTTGCTGGGACAATGGAGATACAGAAAAGATGAGTCCTTGGGATATGGAGCTTATACCTAATAATGCTGTATTTCCTGAAGAACTAGGTACCAGTGTTCCTTTAACTGATGGTGAGTGCAGATCACTAATCTATAAACCTCTTGATGGAGAATGGGGTACCAATCCCAGGGATGAAGAATGTGAAAGAATTGTGGCAGGAATAAACCAGTTGATGACACTAGATATTGCCTCAGCATTTGTGGCCCCCGTGGATCTGCAAGCCTATCCCATGTATTGCACAGTAGTGGCATATCCAACGGATCTAAGTACAATTAAACAAAGACTGGAAAACAGGTTTTACAGGCGGGTTTCTTCCCTAATGTGGGAAGTTCGATATATAGAGCATAATACACGAACATTTAATGAGCCTGGAAGCCCTATTGTGAAATCTGCTAAATTCGTGACTGATCTTCTTCTACATTTTATAAAGGATCAGACTTGTTATAACATAATTCCACTTTATAATTCAATGAAGAAGAAAGTTTTGTCTGATTCTGAGGATGAAGAGAAAGATGCTGATGTGCCAGGAACTTCTACTCGAAAAAGGAAGGACCATCAGCCTAGAAGAAGATTACGTAATAGAGCCCAGTCTTACGATATTCAAGCATGGAAGAAACAGTGTGAAGAATTGTTAAATCTCATATTTCAATGTGAAGATTCAGAGCCTTTCCGTCAGCCGGTAGATCTCCTTGAATATCCAGACTACAGAGACATCATTGACACTCCAATGGATTTTGCTACCGTTAGAGAAACTTTAGAGGCTGGGAATTATGAGTCACCAATGGAGTTATGTAAAGATGTCAGACTTATTTTCAGTAATTCCAAAGCATATACACCAAGCAAAAGATCAAGGATTTACAGCATGAGTTTGCGCTTGTCTGCATTCTTTGAAGAACACATTAGTTCAGTTTTATCAGATTATAAATCTGCTCTTCGTTTTCATAAAAGAAATACCATAACCAAAAGGAGGAAGAAAAGAAACAGAAGCAGCTCTGTTTCCAGTAGTGCTGCATCAAGCCCTGAAAGGAAAAAAAGGATCTTAAAACCCCAGCTAAAATCAGAAAGCTCTACCTCTGCATTCTCTACACCTACACGATCAATACCGCCAAGACACAATGCTGCTCAGATAAACGGTAAAACAGAATCTAGTTCTGTGGTTCGAACCAGAAGCAACCGAGTGGTTGTAGATCCAGTTGTCACTGAGCAACCATCTACTTCTTCAGCTGCAAAGACTTTTATTACAAAAGCTAATGCATCTGCAATACCAGGGAAAACAATACTAGAGAATTCTGTGAAACATTCCAAAGCTTTGAATACTCTTTCCAGTCCTGGTCAATCCAGTTTTAGTCATGGCACTAGGAATAATTCTGCAAAAGAAAACATGGAAAAGGAAAAGCCAGTCAAACGTAAAATGAAGTCATCTGTACTCCCAAAGGCGTCCACTCTTTCAAAGTCATCAGCTGTCATTGAGCAAGGAGATTGTAAGAACAACGCTCTTGTACCAGGAACCATTCAAGTAAATGGCCATGGAGGACAGCCATCAAAACTTGTGAAGAGGGGACCTGGAAGGAAACCTAAAGTAGAAGTTAATACCAATAGTGGTGAAATTATACACAAGAAAAGGGGTAGAAAGCCCAAAAAGCTACAGTATGCAAAGCCAGAAGATTTAGAGCAAAATAATGTGCATCCCATCAGAGATGAAGTACTTCCTTCTTCAACATGCAATTTTCTTTCTGAAACTAATAATGTAAAGGAAGATTTGTTACAGAAAAAGAATCGTGGAGGTAGGAAGCCCAAAAGGAAGATGAAGACACAAAAATTAGATGCAGATCTCCTAGTCCCTGCAAGTGTCAAAGTGTTAAGGAGAAGTAACCGAAAAAAGATAGATGATCCTATAGATGAGGAAGAAGAGTTTGAAGAACTCAAAGGCTCTGAACCCCACATGAGAACTAGAAATCAAGGTCGAAGGACAGCTTTCTATAATGAGGATGACTCTGAAGAGGAGCAAAGGCAGCTGTTGTTCGAAGACACCTCTTTAACTTTTGGAACTTCTAGTAGAGGACGAGTCCGAAAGTTGACTGAAAAAGCAAAAGCTAATTTAATTGGTTGGGCGGCCGCC

Amino Acid Sequence

MDYKDDDDKGSMSCERKGLSELRSELYFLIARFLEDGPCQQAAQVLIREVAEKELLPRRTDWTGKEHPRTYQNLVKYYRHLAPDHLLQICHRLGPLLEQEIPQSVPGVQTLLGAGRQSLLRTNKSCKHVVWKGSALAALHCGRPPESPVNYGSPPSIADTLFSRKLNGKYRLERLVPTAVYQHMKMHKRILGHLSSVYCVTFDRTGRRIFTGSDDCLVKIWATDDGRLLATLRGHAAEISDMAVNYENTMIAAGSCDKMIRVWCLRTCAPLAVLQGHSASITSLQFSPLCSGSKRYLSSTGADGTICFWLWDAGTLKINPRPAKFTERPRPGVQMICSSFSAGGMFLATGSTDHIIRVYFFGSGQPEKISELEFHTDKVDSIQFSNTSNRFVSGSRDGTARIWQFKRREWKSILLDMATRPAGQNLQGIEDKITKMKVTMVAWDRHDNTVITAVNNMTLKVWNSYTGQLIHVLMGHEDEVFVLEPHPFDPRVLFSAGHDGNVIVWDLARGVKIRSYFNMIEGQGHGAVFDCKCSPDGQHFACTDSHGHLLIFGFGSSSKYDKIADQMFFHSDYRPLIRDANNFVLDEQTQQAPHLMPPPFLVDVDGNPHPSRYQRLVPGRENCREEQLIPQMGVTSSGLNQVLSQQANQEISPLDSMIQRLQQEQDLRRSGEAGISNTSRLSRGSISSTSEVHSPPNVGLRRSGQIEGVRQMHSNAPRSEIATERDLVAWSRRVVVPELSAGVASRQEEWRTAKGEEEIKTYRSEEKRKHLTVPKENKIPTVSKNHAHEHFLDLGESKKQQTNQHNYRTRSALEETPRPSEEIENGSSSSDEGEVVAVSGGTSEEEERAWHSDGSSSDYSSDYSDWTADAGINLQPPKKVPKNKTKKAESSSDEEEESEKQKQKQIKKEKKKVNEEKDGPISPKKKKPKERKQKRLAVGELTENGLTLEEWLPSTWITDTIPRRCPFVPQMGDEVYYFRQGHEAYVEMARKNKIYSINPKKQPWHKMELREQELMKIVGIKYEVGLPTLCCLKLAFLDPDTGKLTGGSFTMKYHDMPDVIDFLVLRQQFDDAKYRRWNIGDRFRSVIDDAWWFGTIESQEPLQPEYPDSLFQCYNVCWDNGDTEKMSPWDMELIPNNAVFPEELGTSVPLTDGECRSLIYKPLDGEWGTNPRDEECERIVAGINQLMTLDIASAFVAPVDLQAYPMYCTVVAYPTDLSTIKQRLENRFYRRVSSLMWEVRYIEHNTRTFNEPGSPIVKSAKFVTDLLLHFIKDQTCYNIIPLYNSMKKKVLSDSEDEEKDADVPGTSTRKRKDHQPRRRLRNRAQSYDIQAWKKQCEELLNLIFQCEDSEPFRQPVDLLEYPDYRDIIDTPMDFATVRETLEAGNYESPMELCKDVRLIFSNSKAYTPSKRSRIYSMSLRLSAFFEEHISSVLSDYKSALRFHKRNTITKRRKKRNRSSSVSSSAASSPERKKRILKPQLKSESSTSAFSTPTRSIPPRHNAAQINGKTESSSVVRTRSNRVVVDPVVTEQPSTSSAAKTFITKANASAIPGKTILENSVKHSKALNTLSSPGQSSFSHGTRNNSAKENMEKEKPVKRKMKSSVLPKASTLSKSSAVIEQGDCKNNALVPGTIQVNGHGGQPSKLVKRGPGRKPKVEVNTNSGEIIHKKRGRKPKKLQYAKPEDLEQNNVHPIRDEVLPSSTCNFLSETNNVKEDLLQKKNRGGRKPKRKMKTQKLDADLLVPASVKVLRRSNRKKIDDPIDEEEEFEELKGSEPHMRTRNQGRRTAFYNEDDSEEEQRQLLFEDTSLTFGTSSRGRVRKLTEKAKANLIGWAAAGGGGGMAQVQLVESGGRLVQAGDSLRLSCAASGRTFSTSAMAWFRQAPGREREFVAAITWTVGNTILGDSVKGRFTISRDRAKNTVDLQMDNLEPEDTAVYYCSARSRGYVLSVLRSVDSYDYWGQGTQVTVSG*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU62338
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Clone contains silent changes at: Thr944 ACA > ACG Leu1410 CTG > TTG Ala1412 GCT > GCA plus listed SNPs V663G and L1093P
• Low Temp: No