cDNA Clone ZNRF1

• Synonyms: NIN283
• Plasmid: pBABED P FLAG aGFP16 ZNRF1
• Parent Plasmid: pBABED P FLAG aGFP16
• Expressed: FLAG-aGFP16-5GLY-ZNRF1
• Genbank: NM_032268.4
• Tag: FLAG
• Species: Human

Sequence of Insert

GGATCCATGGGGGGCAAGCAGAGCACGGCGGCCCGCTCCCGGGGCCCCTTCCCGGGGGTCTCCACCGATGACAGCGCCGTGCCGCCGCCGGGAGGGGCGCCCCATTTCGGGCACTACCGGACGGGCGGCGGGGCCATGGGGCTGCGCAGCCGCTCGGTCAGCTCGGTGGCAGGCATGGGCATGGACCCCAGCACGGCCGGGGGGGTGCCCTTTGGCCTCTACACCCCCGCCTCCCGGGGCACCGGCGACTCCGAGAGGGCGCCCGGCGGCGGAGGGTCTGCGTCCGACTCCACCTATGCCCATGGCAATGGTTACCAGGAGACGGGCGGCGGTCACCATAGAGACGGGATGCTGTACCTGGGCTCCCGAGCCTCGCTGGCGGATGCTCTACCTCTGCACATCGCACCCAGGTGGTTCAGCTCGCATAGTGGTTTCAAGTGCCCCATTTGCTCCAAGTCTGTGGCTTCTGACGAGATGGAAATGCACTTTATAATGTGTTTGAGCAAACCTCGCCTCTCCTACAACGATGATGTGCTGACTAAAGACGCGGGTGAGTGTGTGATCTGCCTGGAGGAGCTGCTGCAGGGGGACACGATAGCCAGGCTGCCCTGCCTGTGCATCTATCACAAAAGCTGCATAGACTCGTGGTTTGAAGTGAACAGATCTTGTCCGGAACACCCTGCGGACTGAGCGGCCGC

Amino Acid Sequence

MDYKDDDDKMAQVQLVESGGRLVQAGDSLRLSCAASGRTFSTSAMAWFRQAPGREREFVAAITWTVGNTILGDSVKGRFTISRDRAKNTVDLQMDNLEPE
DTAVYYCSARSRGYVLSVLRSVDSYDYWGQGTQVTVSGGGGGGGSMGGKQSTAARSRGPFPGVSTDDSAVPPPGGAPHFGHYRTGGGAMGLRSRSVSSVA
GMGMDPSTAGGVPFGLYTPASRGTGDSERAPGGGGSASDSTYAHGNGYQETGGGHHRDGMLYLGSRASLADALPLHIAPRWFSSHSGFKCPICSKSVASD
EMEMHFIMCLSKPRLSYNDDVLTKDAGECVICLEELLQGDTIARLPCLCIYHKSCIDSWFEVNRSCPEHPAD*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU64431
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Grow at 30oC, construct prone to rearrangement. Sequence data poor and will require maxiprep quality DNA to finalise sequence.
• Low Temp: Yes