cDNA Clone DCAF8L2

• Synonyms: DDB1 and CUL4 associated factor 8 like 2 , WDR42C
• Plasmid: pFBDMb-6His_TEV DCAF8L2 M1-Q594
• Parent Plasmid: pFBDMb-6His_TEV
• Expressed: 6His_TEV DCAF8L2 M1-Q594
• Genbank: NM_001353448.2 → NP_001340377.1
• Tag: 6His
• Species: Human

Amino Acid Sequence

MHHHHHHENLYFQGGMSHQEGSTDGLPDLGTESLFSSPEEQSGAVAATEASSDIDIATSELSVTVTGDGSDSRDGGFPNDASTENRSSDQESASEDIELESLEDFEHFLMSGESLFHYPLVGEEETEREEEDEEIQEEGGEEEEEEEEEEEEEEEEEEEQPRAGPQGSGGNHEQYSLEEDQALEEWVSSETSALPRPRWQVVTALHQRQLGSRPRFVYEACGARAFVQRFRLQYRLADHVGCVNTVHFNQRGTRLASSGDDLKVIVWDWVRQRPVLNFESGHTNNVFQAKFLPNCGDSTLAMCARDGQVRVAELINASYFNNTKCVAQHRGPAHKLALEPDSPYKFLTSGEDAVVFAIDLRQDRPASKVVVTRENDKKVGLYTITVNPANTYQFAVGGQDQFVRIYDQRKIDKKENNGVLKKFTPHHLVNCDFPTNITCVVYSHDGTELLASYNDDDIYLFNSSHSDGAQYSKRFKGHRNNTTVKGVNFYGPRSEFVVSGSDCGHIFFWEKSSCQIIQFLKGSREGTINCLEPHPYLPVLACSGLDHDVKIWTPTAKAATELTGLKKVIKKNKWERDEDSLHHGSLFDQYMLWFLLRHVTQRGRHQ*

• Vector Type: Baculovirus
• Antibiotic: Amp
• Full Snapgene Map: pFBDMb-6His_TEV DCAF8L2 M1-Q594 1.4 MBSnapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU65663
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: PCR product incorporated into Bam-Not cut pFBDMb-6His_TEV vector 65584 by NEB builder.; Clone harbours Thr345Ala listed SNP; **Clone contains a 3 residue Glu deletion in a highly repetitive region between aa140-145 compared to NCBI ref***
• Low Temp: No