Synonyms
HRCA1, RCA11, pVHL, VHL, aGFP, anti-GFP, GFP binder
Plasmid
pRETROX TIGHT Hygro VHL aGFP16
Parent Plasmid
pRetroX tight puro
Expressed
VHL-5xGLY-aGFP16
Genbank
NM_000551.3
Species
Human
Sequence of Insert
View Sequence
VHL
GGATCCGCCATGCCCCGGAGGGCGGAGAACTGGGACGAGGCCGAGGTAGGCGCGGAGGAGGCAGGCGTCGAAGAGTACGGCCCTGAAGAAGACGGCGGGGAGGAGTCGGGCGCCGAGGAGTCCGGCCCGGAAGAGTCCGGCCCGGAGGAACTGGGCGCCGAGGAGGAGATGGAGGCCGGTCGGCCGCGGCCCGTGCTGCGCTCGGTGAACTCGCGCGAGCCCTCCCAGGTCATCTTCTGCAATCGCAGTCCGCGCGTCGTGCTGCCCGTATGGCTCAACTTCGACGGCGAGCCGCAGCCCTACCCAACGCTGCCGCCTGGCACGGGCCGCCGCATCCACAGCTACCGAGGTCACCTTTGGCTCTTCAGAGATGCAGGGACACACGATGGGCTTCTGGTTAACCAAACTGAATTATTTGTGCCATCTCTCAATGTTGACGGACAGCCTATTTTTGCCAATATCACACTGCCAGTGTATACTCTGAAAGAGCGATGCCTCCAGGTTGTCCGGAGCCTAGTCAAGCCTGAGAATTACAGGAGACTGGACATCGTCAGGTCGCTCTACGAAGATCTGGAAGACCACCCAAATGTGCAGAAAGACCTGGAGCGGCTGACACAGGAGCGCATTGCACATCAACGGATGGGAGATGGTGGAGGCGGAGGT
aGFP16
GAATTCGCCATGGCGCAAGTCCAGCTGGTTGAGTCAGGTGGCCGTTTAGTGCAAGCTGGCGATTCCCTGCGCTTGAGCTGTGCAGCGTCAGGACGCACGTTCTCGACTAGCGCAATGGCCTGGTTTCGCCAAGCACCAGGTCGTGAACGCGAGTTTGTAGCCGCGATTACGTGGACCGTTGGGAACACGATTCTGGGCGATTCGGTGAAAGGGCGTTTCACCATCAGTCGCGATCGTGCCAAAAACACCGTCGATCTCCAGATGGACAATCTGGAACCGGAAGATACAGCGGTGTATTACTGCTCGGCTCGTTCTCGGGGTTATGTGCTGTCCGTTTTACGCAGTGTAGACAGCTACGACTATTGGGGACAGGGCACTCAGGTGACCGTTTCTGGCTGAGCGGCCGC
GGATCCGCCATGCCCCGGAGGGCGGAGAACTGGGACGAGGCCGAGGTAGGCGCGGAGGAGGCAGGCGTCGAAGAGTACGGCCCTGAAGAAGACGGCGGGGAGGAGTCGGGCGCCGAGGAGTCCGGCCCGGAAGAGTCCGGCCCGGAGGAACTGGGCGCCGAGGAGGAGATGGAGGCCGGTCGGCCGCGGCCCGTGCTGCGCTCGGTGAACTCGCGCGAGCCCTCCCAGGTCATCTTCTGCAATCGCAGTCCGCGCGTCGTGCTGCCCGTATGGCTCAACTTCGACGGCGAGCCGCAGCCCTACCCAACGCTGCCGCCTGGCACGGGCCGCCGCATCCACAGCTACCGAGGTCACCTTTGGCTCTTCAGAGATGCAGGGACACACGATGGGCTTCTGGTTAACCAAACTGAATTATTTGTGCCATCTCTCAATGTTGACGGACAGCCTATTTTTGCCAATATCACACTGCCAGTGTATACTCTGAAAGAGCGATGCCTCCAGGTTGTCCGGAGCCTAGTCAAGCCTGAGAATTACAGGAGACTGGACATCGTCAGGTCGCTCTACGAAGATCTGGAAGACCACCCAAATGTGCAGAAAGACCTGGAGCGGCTGACACAGGAGCGCATTGCACATCAACGGATGGGAGATGGTGGAGGCGGAGGT
aGFP16
GAATTCGCCATGGCGCAAGTCCAGCTGGTTGAGTCAGGTGGCCGTTTAGTGCAAGCTGGCGATTCCCTGCGCTTGAGCTGTGCAGCGTCAGGACGCACGTTCTCGACTAGCGCAATGGCCTGGTTTCGCCAAGCACCAGGTCGTGAACGCGAGTTTGTAGCCGCGATTACGTGGACCGTTGGGAACACGATTCTGGGCGATTCGGTGAAAGGGCGTTTCACCATCAGTCGCGATCGTGCCAAAAACACCGTCGATCTCCAGATGGACAATCTGGAACCGGAAGATACAGCGGTGTATTACTGCTCGGCTCGTTCTCGGGGTTATGTGCTGTCCGTTTTACGCAGTGTAGACAGCTACGACTATTGGGGACAGGGCACTCAGGTGACCGTTTCTGGCTGAGCGGCCGC
Amino Acid Sequence
View Sequence
MPRRAENWDEAEVGAEEAGVEEYGPEEDGGEESGAEESGPEESGPEELGAEEEMEAGRPRPVLRSVNSREPSQVIFCNRSPRVVLPVWLNFDGEPQPYPT
LPPGTGRRIHSYRGHLWLFRDAGTHDGLLVNQTELFVPSLNVDGQPIFANITLPVYTLKERCLQVVRSLVKPENYRRLDIVRSLYEDLEDHPNVQKDLER
LTQERIAHQRMGDGGGGGEFAMAQVQLVESGGRLVQAGDSLRLSCAASGRTFSTSAMAWFRQAPGREREFVAAITWTVGNTILGDSVKGRFTISRDRAKN
TVDLQMDNLEPEDTAVYYCSARSRGYVLSVLRSVDSYDYWGQGTQVTVSG*
LPPGTGRRIHSYRGHLWLFRDAGTHDGLLVNQTELFVPSLNVDGQPIFANITLPVYTLKERCLQVVRSLVKPENYRRLDIVRSLYEDLEDHPNVQKDLER
LTQERIAHQRMGDGGGGGEFAMAQVQLVESGGRLVQAGDSLRLSCAASGRTFSTSAMAWFRQAPGREREFVAAITWTVGNTILGDSVKGRFTISRDRAKN
TVDLQMDNLEPEDTAVYYCSARSRGYVLSVLRSVDSYDYWGQGTQVTVSG*
Vector Type
Mammalian
Antibiotic
Amp
Full Snapgene Map
pRETROX TIGHT Hygro VHL aGFP16113.97 KBSnapgene files can be opened using software available here: www.snapgene.com
DU Number
DU57840
Unit Source
Location
PPU
MTA
Notes
Designed to allow tet-inducible control of degron expression. Contains aGFP16, academic use only.
Swapped Puro in 54388 vector for Hygro.
Swapped Puro in 54388 vector for Hygro.
Low Temp
No
Price
£150.00
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