cDNA Clone VHL

• Synonyms: HRCA1, RCA11, pVHL, VHL, aGFP, anti-GFP, GFP binder
• Plasmid: pRETROX TIGHT Puro VHL LAG16
• Parent Plasmid: pRetroX tight puro
• Expressed: VHL-5xGLY-aGFP.16
• Genbank: NM_000551.3
• Species: Human

Sequence of Insert

GGATCCGCCATGCCCCGGAGGGCGGAGAACTGGGACGAGGCCGAGGTAGGCGCGGAGGAGGCAGGCGTCGAAGAGTACGGCCCTGAAGAAGACGGCGGGGAGGAGTCGGGCGCCGAGGAGTCCGGCCCGGAAGAGTCCGGCCCGGAGGAACTGGGCGCCGAGGAGGAGATGGAGGCCGGTCGGCCGCGGCCCGTGCTGCGCTCGGTGAACTCGCGCGAGCCCTCCCAGGTCATCTTCTGCAATCGCAGTCCGCGCGTCGTGCTGCCCGTATGGCTCAACTTCGACGGCGAGCCGCAGCCCTACCCAACGCTGCCGCCTGGCACGGGCCGCCGCATCCACAGCTACCGAGGTCACCTTTGGCTCTTCAGAGATGCAGGGACACACGATGGGCTTCTGGTTAACCAAACTGAATTATTTGTGCCATCTCTCAATGTTGACGGACAGCCTATTTTTGCCAATATCACACTGCCAGTGTATACTCTGAAAGAGCGATGCCTCCAGGTTGTCCGGAGCCTAGTCAAGCCTGAGAATTACAGGAGACTGGACATCGTCAGGTCGCTCTACGAAGATCTGGAAGACCACCCAAATGTGCAGAAAGACCTGGAGCGGCTGACACAGGAGCGCATTGCACATCAACGGATGGGAGATGGTGGAGGCGGAGGTGAATTCGCCATGGCGCAAGTCCAGCTGGTTGAGTCAGGTGGCCGTTTAGTGCAAGCTGGCGATTCCCTGCGCTTGAGCTGTGCAGCGTCAGGACGCACGTTCTCGACTAGCGCAATGGCCTGGTTTCGCCAAGCACCAGGTCGTGAACGCGAGTTTGTAGCCGCGATTACGTGGACCGTTGGGAACACGATTCTGGGCGATTCGGTGAAAGGGCGTTTCACCATCAGTCGCGATCGTGCCAAAAACACCGTCGATCTCCAGATGGACAATCTGGAACCGGAAGATACAGCGGTGTATTACTGCTCGGCTCGTTCTCGGGGTTATGTGCTGTCCGTTTTACGCAGTGTAGACAGCTACGACTATTGGGGACAGGGCACTCAGGTGACCGTTTCTGGCTGAGCGGCCGC

Amino Acid Sequence

MPRRAENWDEAEVGAEEAGVEEYGPEEDGGEESGAEESGPEESGPEELGAEEEMEAGRPRPVLRSVNSREPSQVIFCNRSPRVVLPVWLNFDGEPQPYPT
LPPGTGRRIHSYRGHLWLFRDAGTHDGLLVNQTELFVPSLNVDGQPIFANITLPVYTLKERCLQVVRSLVKPENYRRLDIVRSLYEDLEDHPNVQKDLER
LTQERIAHQRMGDGGGGGEFAMAQVQLVESGGRLVQAGDSLRLSCAASGRTFSTSAMAWFRQAPGREREFVAAITWTVGNTILGDSVKGRFTISRDRAKN
TVDLQMDNLEPEDTAVYYCSARSRGYVLSVLRSVDSYDYWGQGTQVTVSG*

• Vector Type: Mammalian
• Antibiotic: Amp
• Full Snapgene Map: pRETROX TIGHT Puro VHL LAG1670.49 KBSnapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU54388
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Designed to allow tet-inducible control of degron expression. Contains aGFP.16, academic use only.
• Low Temp: No