cDNA Clone ULK1

• Synonyms: FLAG-ULK1_S2A_561_6922_2R18
• Plasmid: pBABE-FLAG-ULK1 S556A, S694A 561_6922_2R18
• Parent Plasmid: pBABE FLAG
• Expressed: FLAG-ULK1_S2A_561_6922_2R18
• Genbank: NM_003565.4 , ENSG00000177169, GI:225637564 c2141t (P714L (exisiting SNP)
• Species: Human

Sequence of Insert

see attached Snapgene file

Amino Acid Sequence

MDYKDDDDKGSLEPGRGGTETVGKFEFSRKDLIGHGAFAVVFKGRHREKHDLEVAVKCINKKNLAKSQTLLGKEIKILKELKHENIVALYDFQEMANSVYLVMEYCNGGDLADYLHAMRTLSEDTIRLFLQQIAGAMRLLHSKGIIHRDLKPQNILLSNPAGRRANPNSIRVKIADFGFARYLQSNMMAATLCGSPMYMAPEVIMSQHYDGKADLWSIGTIVYQCLTGKAPFQASSPQDLRLFYEKNKTLVPTIPRETSAPLRQLLLALLQRNHKDRMDFDEFFHHPFLDASPSVRKSPPVPVPSYPSSGSGSSSSSSSTSHLASPPSLGEMQQLQKTLASPADTAGFLHSSRDSGGSKDSSCDTDDFVMVPAQFPGDLVAEAPSAKPPPDSLMCSGSSLVASAGLESHGRTPSPSPPCSSSPSPSGRAGPFSSSRCGASVPIPVPTQVQNYQRIERNLQSPTQFQTPRSSAIRRSGSTSPLGFARASPSPPAHAEHGGVLARKMSLGGGRPYTPSPQVGTIPERPGWSGTPSPQGAEMRGGRSPRPGSSAPEHSPRTSGLGCRLHAAPNLNSPHCVPRDLSWLDLEANMCLPSDLHVVRPKLPKPPTDPLGAVFSPPQASPPQPSHGLQSCRNLRGSPKLPDFLQRNPLPPILGSPTKAVPSFDFPKTPSSQNLLALLARQGVVMTPPRNRTLPDLSEVGPFHGQPLGPGLRPGEDPKGPFGRNSPHCVPRDLSWLDLEANMCLPSFATSRLTDLLLKAAFGTQAPDLGSTESLQEKPMEIAPSAGFGGSLHPGARAGGTSSPSPVVFTVGSPPSGSTPPQGPRTRMFSAGPTGSASSSARHLVPGPCSEAPAPELPAPGHGCSFADPIAANLEGAVTFEAPDLPEETLMEQEHTEILRGLRFTLLFVQHVLEIAALKGSASEAAGGPEYQLQESVVADQISLLSREWGFAEQLVLYLKVAELLSSGLQSAIDQIRAGKLCLSSTVKQVVRRLNELYKASVVSCQGLSLRLQRFFLDKQRLLDRIHSITAERLIFSHAVQMVQSAALDEMFQHREGCVPRYHKALLLLEGLQHMLSDQADIENVTKCKLCIERRLSALLTGICA*

• Vector Type: Mammalian
• Full Snapgene Map: pBABE-FLAG-ULK1 S556A, S694A 561_6922_2R18619.13 KBSnapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU79128
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: peptide sequence (NSPHCVPRDLSWLDLEANMCLP) inserted at two sites in-frame at amino acid site 561 (in between Leu 560 and Serine 561) and site 692 (in between Arg 691 and Serine 692); Grow at or below 30ºC
• Low Temp: Yes