cDNA Clone AMPKA2; AMPKB2; AMPKG1

• Synonyms: PRKAA2
• Plasmid: pOPC 6His AMPKA2; AMPKB2; AMPKG1
• Parent Plasmid: pOPC
• Expressed: 6His AMPKA2; AMPKB2; AMPKG1
• Genbank: NP_006242.5 NP_005390.1 AAH00358.1
• Tag: 6His
• Species: Human

Sequence of Insert

DNA sequence of AMPKA2 insert
TCTAGAAATAATTTGTTTAACTTTAAGAAGGAGATATACATATGCATCAT
CACCATCACCATGCTGAGAAGCAGAAGCACGACGGGCGGGTGAAGATCGG
ACACTACGTGCTGGGCGACACGCTGGGCGTCGGCACCTTCGGCAAAGTGA
AGATTGGAGAACATCAATTAACAGGCCATAAAGTGGCAGTTAAAATCTTA
AATAGACAGAAGATTCGCAGTTTAGATGTTGTTGGAAAAATAAAACGAGA
AATTCAAAATCTAAAACTCTTTCGTCATCCTCATATTATCAAACTATACC
AGGTGATCAGCACTCCAACAGATTTTTTTATGGTAATGGAATATGTGTCT
GGAGGTGAATTATTTGACTACATCTGTAAGCATGGACGGGTTGAAGAGAT
GGAAGCCAGGCGGCTCTTTCAGCAGATTCTGTCTGCTGTGGATTACTGTC
ATAGGCATATGGTTGTTCATCGAGACCTGAAACCAGAGAATGTCCTGTTG
GATGCACACATGAATGCCAAGATAGCCGATTTCGGATTATCTAATATGAT
GTCAGATGGTGAATTTCTGAGAACTAGTTGCGGATCTCCAAATTATGCAG
CACCTGAAGTCATCTCAGGCAGATTGTATGCAGGTCCTGAAGTTGATATC
TGGAGCTGTGGTGTTATCTTGTATGCTCTTCTTTGTGGCACCCTCCCATT
TGATGATGAGCATGTACCTACGTTATTTAAGAAGATCCGAGGGGGTGTCT
TTTATATCCCAGAATATCTCAATCGTTCTGTCGCCACTCTCCTGATGCAT
ATGCTGCAGGTTGACCCACTGAAACGAGCAACTATCAAAGACATAAGAGA
GCATGAATGGTTTAAACAAGATTTGCCCAGTTACTTATTTCCTGAAGACC
CTTCCTATGATGCTAACGTCATTGATGATGAGGCTGTGAAAGAAGTGTGT
GAAAAATTTGAATGTACAGAATCAGAAGTAATGAACAGTTTATATAGTGG
TGACCCTCAAGACCAGCTTGCAGTGGCTTATCATCTTATCATTGACAATC
GGAGAATAATGAACCAAGCCAGTGAGTTCTACCTCGCCTCTAGTCCTCCA
TCTGGTTCTTTTATGGATGATAGTGCCATGCATATTCCCCCAGGCCTGAA
ACCTCATCCAGAAAGGATGCCACCTCTTATAGCAGACAGCCCCAAAGCAA
GATGTCCATTGGATGCACTGAATACGACTAAGCCCAAATCTTTAGCTGTG
AAAAAAGCCAAGTGGCATCTTGGAATCCGAAGTCAGAGCAAACCGTATGA
CATTATGGCTGAAGTTTACCGAGCTATGAAGCAGCTGGATTTTGAATGGA
AGGTAGTGAATGCATACCATCTTCGTGTAAGAAGAAAAAATCCAGTGACT
GGCAATTACGTGAAAATGAGCTTACAACTTTACCTGGTTGATAACAGGAG
CTATCTTTTGGACTTTAAAAGCATTGATGATGAAGTAGTGGAGCAGAGAT
CTGGTTCCTCAACACCTCAGCGTTCCTGTTCTGCTGCTGGCTTACACAGA
CCAAGATCAAGTTTTGATTCCACAACTGCAGAGAGCCATTCACTTTCTGG
CTCTCTCACTGGCTCTTTGACCGGAAGCACATTGTCTTCAGTTTCACCTC
GCCTGGGCAGTCACACCATGGATTTTTTTGAAATGTGTGCCAGTCTGATT
ACTACTTTAGCCCGTTGAGAATCC

DNA sequence of AMPKB2 insert
GGAATTCAATAATTTTGTTAACTTTAAGAAGGAGATATACATATGGGAAA
CACCACCAGCGACCGGGTGTCCGGGGAGCGCCACGGCGCCAAGGCTGCAC
GCTCCGAGGGCGCAGGCGGCCATGCCCCGGGGAAGGAGCACAAGATCATG
GTGGGGAGTACGGACGACCCCAGCGTGTTCAGCCTCCCTGACTCCAAGCT
CCCTGGGGACAAAGAGTTTGTATCATGGCAGCAGGATTTGGAGGACTCCG
TAAAGCCCACACAGCAGGCCCGGCCCACTGTTATCCGCTGGTCTGAAGGA
GGCAAGGAGGTCTTCATCTCTGGGTCCTTCAACAATTGGAGCACCAAGAT
TCCACTGATTAAGAGCCATAATGACTTTGTTGCCATCCTGGACCTCCCTG
AGGGAGAGCACCAATACAAGTTCTTTGTGGATGGACAGTGGGTTCATGAT
CCATCAGAGCCTGTGGTTACCAGTCAGCTTGGCACAATTAACAATTTGAT
CCATGTCAAGAAATCTGATTTTGAGGTGTTCGATGCTTTAAAGTTAGATT
CTATGGAAAGTTCTGAGACATCTTGTAGAGACCTTTCCAGCTCACCCCCA
GGGCCTTATGGTCAAGAAATGTATGCGTTTCGATCTGAGGAAAGATTCAA
ATCCCCACCCATCCTTCCTCCTCATCTACTTCAAGTTATTCTTAACAAAG
ACACTAATATTTCTTGTGACCCAGCCTTACTCCCTGAGCCCAACCATGTT
ATGCTGAACCATCTCTATGCATTGTCCATTAAGGACAGTGTGATGGTCCT
TAGCGCAACCCATCGCTACAAGAAGAAGTATGTTACTACTCTGCTATACA
AGCCCATTTGAAAGCTT

DNA sequence of AMPKG1 insert
GAGCTCAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGGAGAC
GGTCATTTCTTCAGATAGCTCCCCAGCTGTGGAAAATGAGCATCCTCAAG
AGACCCCAGAATCCAACAATAGCGTGTATACTTCCTTCATGAAGTCTCAT
CGCTGCTATGACCTGATTCCCACAAGCTCCAAATTGGTTGTATTTGATAC
GTCCCTGCAGGTGAAGAAAGCTTTTTTTGCTTTGGTGACTAACGGTGTAC
GAGCTGCCCCTTTATGGGATAGTAAGAAGCAAAGTTTTGTGGGCATGCTG
ACCATCACTGATTTCATCAATATCCTGCACCGCTACTATAAATCAGCCTT
GGTACAGATCTATGAGCTAGAAGAACACAAGATAGAAACTTGGAGAGAGG
TGTATCTCCAGGACTCCTTTAAACCGCTTGTCTGCATTTCTCCTAATGCC
AGCTTGTTTGATGCTGTCTCTTCATTAATTCGGAACAAGATCCACAGGCT
GCCAGTTATTGACCCAGAATCAGGCAATACTTTGTACATCCTCACCCACA
AGCGCATTCTGAAGTTCCTCAAATTGTTTATCACTGAGTTCCCCAAGCCA
GAGTTCATGTCCAAGTCTCTGGAAGAGCTACAGATTGGCACCTATGCCAA
TATTGCTATGGTTCGCACTACCACCCCCGTCTATGTGGCTCTGGGGATTT
TTGTACAGCATCGAGTCTCAGCCCTGCCAGTGGTGGATGAGAAGGGGCGT
GTGGTGGACATCTACTCCAAGTTTGATGTTATCAATCTGGCAGCAGAAAA
GACCTACAACAACCTAGATGTATCTGTGACTAAAGCCTTGCAACATCGAT
CACATTACTTTGAGGGTGTTCTCAAGTGCTACCTGCATGAGACTCTGGAG
ACCATCATCAACAGGCTAGTGGAAGCAGAGGTTCACCGACTTGTAGTGGT
GGATGAAAATGATGTGGTCAAGGGAATTGTATCACTGTCTGACATCCTGC
AGGCCCTGGTGCTCACAGGTGGAGAGAAGAAGCCCTGAGGTACC

Amino Acid Sequence

Protein sequence of AMPKA2
MHHHHHHAEKQKHDGRVKIGHYVLGDTLGVGTFGKVKIGEHQLTGHKVAV
KILNRQKIRSLDVVGKIKREIQNLKLFRHPHIIKLYQVISTPTDFFMVME
YVSGGELFDYICKHGRVEEMEARRLFQQILSAVDYCHRHMVVHRDLKPEN
VLLDAHMNAKIADFGLSNMMSDGEFLRTSCGSPNYAAPEVISGRLYAGPE
VDIWSCGVILYALLCGTLPFDDEHVPTLFKKIRGGVFYIPEYLNRSVATL
LMHMLQVDPLKRATIKDIREHEWFKQDLPSYLFPEDPSYDANVIDDEAVK
EVCEKFECTESEVMNSLYSGDPQDQLAVAYHLIIDNRRIMNQASEFYLAS
SPPSGSFMDDSAMHIPPGLKPHPERMPPLIADSPKARCPLDALNTTKPKS
LAVKKAKWHLGIRSQSKPYDIMAEVYRAMKQLDFEWKVVNAYHLRVRRKN
PVTGNYVKMSLQLYLVDNRSYLLDFKSIDDEVVEQRSGSSTPQRSCSAAG
LHRPRSSFDSTTAESHSLSGSLTGSLTGSTLSSVSPRLGSHTMDFFEMCA
SLITTLAR*

Protein sequence AMPKB2
MGNTTSDRVSGERHGAKAARSEGAGGHAPGKEHKIMVGSTDDPSVFSLPD
SKLPGDKEFVSWQQDLEDSVKPTQQARPTVIRWSEGGKEVFISGSFNNWS
TKIPLIKSHNDFVAILDLPEGEHQYKFFVDGQWVHDPSEPVVTSQLGTIN
NLIHVKKSDFEVFDALKLDSMESSETSCRDLSSSPPGPYGQEMYAFRSEE
RFKSPPILPPHLLQVILNKDTNISCDPALLPEPNHVMLNHLYALSIKDSV
MVLSATHRYKKKYVTTLLYKPI*

Protein sequence AMPKG1
METVISSDSSPAVENEHPQETPESNNSVYTSFMKSHRCYDLIPTSSKLVV
FDTSLQVKKAFFALVTNGVRAAPLWDSKKQSFVGMLTITDFINILHRYYK
SALVQIYELEEHKIETWREVYLQDSFKPLVCISPNASLFDAVSSLIRNKI
HRLPVIDPESGNTLYILTHKRILKFLKLFITEFPKPEFMSKSLEELQIGT
YANIAMVRTTTPVYVALGIFVQHRVSALPVVDEKGRVVDIYSKFDVINLA
AEKTYNNLDVSVTKALQHRSHYFEGVLKCYLHETLETIINRLVEAEVHRL
VVVDENDVVKGIVSLSDILQALVLTGGEKKP*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU32692
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression vector; ; Estimated MW of AMPKA2 : 63140 Daltons; Estimated MW of AMPKB2 : 30301 Daltons; Estimated MW of AMPKG1 : 37578 Daltons
• Low Temp: No