cDNA Clone Ufl1

• Plasmid: petDUET1 6His TEV Ufl1(1-410)(C32A)/StrepII-C3-DDRGK1(A29-end)
• Parent Plasmid: pETDuet1
• Expressed: His6-TEV-Ufl1(1-410)(C32A)/StrepII-C3-DDRGK1(A29-end)
• Genbank: NM_023935.2
• Tag: 6His
• Species: Human

Sequence of Insert

CCATGGGACACCATCACCATCACCATGAAAACCTGTATTTTCAGATGGCGGACGCCTGGGAAGAGATTAGGCGGTTGGCGGCCGACTTCCAGCGGGCGCAGTTCGCCGAGGCCACGCAGAGGTTGTCCGAGCGGAACGCCATTGAGATTGTTAATAAATTGATTGCTCAGAAACAGCTAGAAGTAGTTCATACACTCGATGGAAAGGAATATATTACTCCAGCCCAAATTAGTAAAGAAATGAGAGATGAGCTACATGTCCGAGGTGGTCGAGTAAACATTGTTGATCTACAACAGGTAATTAATGTGGACCTGATTCATATTGAAAATAGAATTGGTGACATTATTAAATCAGAAAAGCATGTTCAGTTAGTGTTGGGACAACTGATAGATGAGAATTATTTGGATCGGTTGGCAGAAGAGGTCAATGATAAATTGCAAGAAAGTGGTCAGGTCACCATATCAGAACTGTGTAAAACTTATGATCTTCCTGGGAACTTTCTGACACAGGCACTAACTCAGCGACTTGGTAGAATTATCAGTGGACATATTGATCTTGATAATAGAGGAGTAATTTTTACGGAAGCTTTTGTAGCTCGACATAAAGCACGTATCCGTGGACTATTCAGTGCTATTACCCGGCCTACAGCTGTGAATTCTTTGATTTCAAAATATGGATTTCAGGAGCAGCTTCTTTACTCTGTGCTTGAGGAACTTGTTAATAGCGGACGCTTACGAGGCACTGTGGTTGGTGGGAGACAGGATAAAGCTGTGTTTGTCCCTGACATCTACTCCAGGACACAGAGTACTTGGGTGGATTCCTTTTTCAGGCAGAATGGCTATCTAGAATTTGATGCTTTGTCCAGACTTGGAATCCCAGATGCTGTAAGCTACATAAAGAAAAGATATAAGACTACACAACTCTTGTTTTTGAAAGCAGCTTGTGTTGGTCAAGGACTTGTGGATCAAGTGGAAGCATCAGTAGAAGAAGCCATCAGCTCTGGAACATGGGTTGATATTGCACCTCTGCTACCCACTTCTTTATCAGTTGAAGATGCTGCCATATTGCTTCAGCAGGTGATGAGGGCATTCAGCAAACAGGCCTCAACTGTAGTCTTTAGCGACACTGTTGTAGTCAGTGAAAAATTTATAAATGACTGTACAGAACTGTTCCGTGAGCTGATGCACCAGAAAGCTGAAAAGGAAATGAAAAATAATCCTGTGCATTTAATCACTGAAGAAGATCTGAAACAAATCTCCACTTTAGAAAGCGTTTAAAGTACAAGTAAAAAGGATAAAAAAGATGAGCGAAGAAGGAAAGCAACAGAGGGCAGTGGAAGCATGAGAGGAGGAGGTGGGGGCAATGCCAGAGAGTACAAAATTAAAAAAGTCAAGAAGAAAGGAAGAAAAGATGATGATAGTGATGATGAATCTCAATCATCCCACACTGGAAAGAAGAAGCCAGAGATCAGTTTTATGTTCCAGGATGAGATTGAAGATTTTTTAAGAAAACACATACAAGATGCCCCTGAGGAGTTTATTTCGGAACTTGCTGAGTACTTAATAAAACCTCTTAATAAAACTTATCTCGAGGTGGTACGTTCAGTATTCATGTCTTCAACAACTTCTGCTTCTGGGACGGGCAGAAAACGCACAATCAAGGACTTGCAAGAAGAAGTTTCAAACCTGTACAATAACATTAGGTTATTTGAAAAAGGGATGAAGTTTTTTGCAGATGACACACAGGCTGCTCTTACCAAACACTTGCTGAAGTCAGTGTGTACTGATATCACTAACCTCATTTTCAACTTCTTAGCTTCGGATTTAATGATGGCAGTAGACGATCCTGCAGCCATTACAAGTGAAATAAGAAAGAAAATTTTAAGTAAATTATCAGAAGAAACCAAAGTAGCTCTTACAAAACTCCATAACTCTCTGAATGAAAAGAGCATAGAAGACTTTATTTCTTGTCTGGATTCTGCAGCAGAAGCTTGTGATATTATGGTGAAAAGGGGAGACAAAAAAAGGGAAAGACAGATACTGTTCCAACATCGACAAGCACTGGCTGAACAGCTAAAGGTCACAGAAGACCCTGCTCTTATTCTGCACCTCACATCAGTCCTGTTGTTTCAGTTTTCAACCCACAGCATGCTCCATGCACCTGGAAGATGTGTCCCACAGATCATTGCTTTTCTTAATAGTAAAATTCCAGAGGATCAGCATGCTCTTTTGGTAAAGTATCAAGGTTTGGTTGTAAAGCAGCTAGTCAGTCAAAGTAAGAAGACTGGGCAGGGAGATTATCCCTTGAATAATGAATTAGACAAAGAACAAGAAGATGTTGCCAGTACTACTCGTAAAGAGCTTCAAGAACTTTCTTCATCCATTAAAGACCTTGTTCTCAAATCTAGGAAATCATCTGTGACGGAAGAGTAAGCGGCCGC

Amino Acid Sequence

MGHHHHHHENLYFQMADAWEEIRRLAADFQRAQFAEATQRLSERNAIEIVNKLIAQKQLEVVHTLDGKEYITPAQISKEMRDELHVRGGRVNIVDLQQVINVDLIHIENRIGDIIKSEKHVQLVLGQLIDENYLDRLAEEVNDKLQESGQVTISELCKTYDLPGNFLTQALTQRLGRIISGHIDLDNRGVIFTEAFVARHKARIRGLFSAITRPTAVNSLISKYGFQEQLLYSVLEELVNSGRLRGTVVGGRQDKAVFVPDIYSRTQSTWVDSFFRQNGYLEFDALSRLGIPDAVSYIKKRYKTTQLLFLKAACVGQGLVDQVEASVEEAISSGTWVDIAPLLPTSLSVEDAAILLQQVMRAFSKQASTVVFSDTVVVSEKFINDCTELFRELMHQKAEKEMKNNPVHLITEEDLKQISTLESV*

• Vector Type: Bacterial
• Antibiotic: Amp
• Full Snapgene Map: petDUET1 6His TEV Ufl1(1-410)(C32A)/StrepII-C3-DDRGK1(A29-end)88.13 KBSnapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU66308
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: SDM of DU66224 with SW5150/1
• Low Temp: No