cDNA Clone FANCE

• Synonyms: FACE; FAE
• Plasmid: pFBDM6P1 FANCE M1-R221/FANCC
• Parent Plasmid: pFBDM6P1
• Expressed: GST 3C FANCE M1-R221
• Genbank: NM_021922.2 GI:66879667 with silent changes a387c and c1116t
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGGCGACACCGGACGCGGGGCTCCCTGGGGCTGAGGGCGTGGAGCCGGCGCCCTGGGCGCAGCTGGAGGCCCCCGCCCGCCTCCTGCTGCAGGCGCTGCAGGCGGGGCCTGAGGGGGCGCGGCGCGGCCTGGGGGTGCTCCGGGCGCTGGGCAGCCGCGGCTGGGAGCCCTTCGACTGGGGTCGCTTGCTCGAGGCCCTGTGCCGGGAGGAGCCGGTCGTGCAGGGGCCTGACGGCCGTCTGGAGCTGAAACCACTGTTGCTGCGATTGCCCCGGATATGCCAGAGGAACCTGATGTCCCTGCTGATGGCCGTTCGGCCATCGCTGCCGGAAAGTGGGCTCCTCTCTGTGCTGCAGATTGCCCAGCAGGACCTAGCCCCTGACCCCGATGCCTGGCTCCGTGCCCTGGGGGAATTGCTGCGAAGGGATTTGGGGGTGGGGACCTCCATGGAGGGAGCTTCTCCACTGTCTGAAAGATGCCAGAGACAGCTCCAAAGTCTATGTAGGGGGCTGGGCCTGGGGGGCAGGAGGTTGAAATCCCCCCAGGCTCCAGACCCTGAAGAAGAGGAGAACAGGGACTCCCAGCAGCCTGGGAAACGCAGAAAGGACTCAGAGGAAGAGGCTGCCAGTCCTGAGGGGAAGAGGGTCCCCAAAAGATTACGGTGATGGGAAGAGGAAGAAGATCATGAGAAGGAGAGACCCGAACATAAGTCACTGGAATCCCTGGCAGATGGAGGAAGTGCATCTCCTATTAAGGACCAGCCTGTCATGGCAGTTAAGACTGGCGAGGACGGTTCGAATCTGGATGATGCTAAAGGTCTGGCTGAGAGTTTGGAGTTGCCCAAAGCTATCCAGGACCAGCTTCCCAGGCTGCAGCAGCTGCTGAAGACCTTGGAGGAGGGGTTAGAGGGATTGGAGGATGCCCCCCCAGTTGAGCTACAGCTTCTTCACGAATGTAGTCCCAGCCAGATGGACTTGCTGTGTGCCCAGCTGCAGCTCCCTCAGCTCTCAGACCTCGGTCTCCTGCGGCTCTGCACCTGGCTGCTGGCCCTTTCACCTGATCTCAGCCTCAGCAATGCTACTGTGCTGACCAGAAGCCTCTTTCTTGGACGGATTCTCTCCTTGACTTCCTCAGCCTCCCGCCTGCTTACAACTGCCCTGACCTCCTTCTGTGCCAAATATACATACCCTGTCTGCAGCGCCCTCCTTGACCCTGTGCTCCAGGCCCCAGGCACAGGTCCTGCTCAAACAGAGTTACTGTGTTGCCTTGTGAAGATGGAGTCCCTGGAGCCAGATGCACAGGTTCTAATGCTGGGACAGATCTTGGAGCTGCCCTGGAAGGAGGAAACTTTCTTGGTGTTGCAGTCACTCCTAGAGCGGCAGGTGGAGATGACCCCTGAGAAGTTCAGTGTCTTAATGGAGAAGCTCTGTAAAAAGGGGCTGGCAGCCACCACCTCCATGGCCTATGCCAAGCTCATGCTGACAGTGATGACCAAGTATCAGGCTAACATCACTGAGACCCAGAGGCTGGGCCTGGCTATGGCCCTAGAACCTAACACCACCTTCCTGAGGAAGTCCCTGAAGGCCGCCTTGAAACATTTGGGCCCCTGAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMATPDAGLPGAEGVEPAPWAQLEAPARLLLQALQAGPEGARRGLGVLRALGSRGWEPFDWGRLLEALCR
EEPVVQGPDGRLELKPLLLRLPRICQRNLMSLLMAVRPSLPESGLLSVLQIAQQDLAPDPDAWLRALGELLRRDLGVGTSMEGASPLSERCQRQLQSLCR
GLGLGGRRLKSPQAPDPEEEENRDSQQPGKRRKDSEEEAASPEGKRVPKRLR*

• Vector Type: Baculovirus
• Antibiotic: Amp
• Full Snapgene Map: pFBDM6P1 FANCE M1-R221/FANCC125.59 KB Snapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU53390
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mutagenesis of DU50454 to introduce stop codon in FANCE sequence.
• Low Temp: No