Synonyms
T05A12.3
Plasmid
pK27 2xFlag-SUMO-CeSPT-2 TEV-His6
Expressed
2xFlag-SUMO-CeSPT-2-TEV-His6
Genbank
NM_068658.6
Vector Type
Bacterial
Antibiotic
Kan
Full Snapgene Map
pK27 2xFlag-SUMO-CeSPT-2 TEV-His652.82 KB
Snapgene files can be opened using software available here: www.snapgene.com
DU Number
DU70523
Unit Source
Location
PPU
MTA
Notes
CeSPT-2 cDNA was amplified with 15nt overhangs complementary to a 2xFlag-SUMO-TEV-His6 vector (DU70522), by perform PCR using primers GSA122 and GSA123 and using DU70516 as template. The 2xFlag-SUMO-TEV-His6 vector (DU70522) was opened and linearized by inverse PCR using primers GSA124 and GSA125, and the two fragments ligated by InFusion cloning.
GSA122 GAACAGATTGGTGGCatggacttctccac
GSA123 CAGGTTTTCactaccCAATTTAACAGAACGTCC
GSA124 ggtagtGAAAACCTGTATTTTCAG
GSA125 GCCACCAATCTGTTCTCTGTG
Corresponds to #120 in GSaredi library.
GSA122 GAACAGATTGGTGGCatggacttctccac
GSA123 CAGGTTTTCactaccCAATTTAACAGAACGTCC
GSA124 ggtagtGAAAACCTGTATTTTCAG
GSA125 GCCACCAATCTGTTCTCTGTG
Corresponds to #120 in GSaredi library.
Low Temp
No
Price
£125.00
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