cDNA Clone UBE2Q2

• Plasmid: pET15b 6His 3C UBE2Q2
• Parent Plasmid: pET15b 6His 3C
• Expressed: 6His 3C UBE2Q2
• Genbank: AAH34342.1 NM_173469.4
• Tag: 6His
• Species: Human

Sequence of Insert

GGATCCATGTCCGTGTCAGGGCTCAAGGCCGAGCTGAAGTTCCTGGCGTC
CATCTTCGACAAGAACCACGAGCGATTCCGCATCGTCAGTTGGAAGCTGG
ACGAGCTGCACTGCCAGTTCCTGGTGCCGCAGCAGGGCAGCCCGCACTCG
CTGCCGCCGCCACTCACGCTCCACTGCAACATCACGGAATCCTATCCATC
TTCTTCACCGATATGGTTTGTGGATTCTGAAGACCCAAATCTGACATCAG
TTCTGGAACGTCTAGAAGATACTAAGAACAACAATTTGCTTCGTCAGCAA
TTGAAGTGGTTGATATGTGAACTCTGCAGTTTATATAACCTTCCTAAGCA
CCTGGATGTTGAGATGCTAGATCAACCACTACCCACGGGTCAGAATGGGA
CAACAGAAGAAGTGACTTCAGAAGAAGAGGAAGAAGAAGAAGAGATGGCT
GAAGATATAGAAGACTTAGATCACTATGAGATGAAGGAAGAAGAGCCTAT
TAGTGGGAAAAAGTCAGAGGATGAAGGAATTGAAAAAGAAAATTTGGCAA
TATTAGAGAAAATTAGGAAGACTCAAAGGCAAGACCATTTAAATGGTGCA
GTGTCTGGGTCAGTGCAAGCTTCAGATAGACTTATGAAAGAGCTCAGGGA
CATATACAGATCACAGAGTTATAAAACAGGGATTTATTCAGTGGAACTCA
TAAATGACAGTTTATATGACTGGCATGTTAAACTGCAGAAGGTTGACCCT
GATAGTCCTTTGCACAGTGATCTTCAGATCTTAAAAGAAAAAGAAGGCAT
AGAATATATTTTGCTTAACTTCTCTTTTAAGGATAACTTTCCATTTGATC
CTCCATTTGTTCGAGTGGTGTTACCTGTTCTCTCAGGAGGGTATGTATTG
GGTGGAGGAGCATTATGTATGGAACTTCTCACAAAACAGGGCTGGAGCAG
TGCCTACTCAATAGAATCGGTCATCATGCAAATAAATGCCACCTTAGTCA
AAGGCAAAGCCAGAGTGCAGTTTGGAGCAAATAAGAATCAATATAATCTA
GCAAGAGCCCAACAATCCTATAATTCCATTGTACAGATACATGAGAAAAA
TGGCTGGTACACCCCTCCAAAGGAAGATGGCTAAGCGGCCGC

Amino Acid Sequence

MGSSHHHHHHSSGLEVLFQGPGSMSVSGLKAELKFLASIFDKNHERFRIV
SWKLDELHCQFLVPQQGSPHSLPPPLTLHCNITESYPSSSPIWFVDSEDP
NLTSVLERLEDTKNNNLLRQQLKWLICELCSLYNLPKHLDVEMLDQPLPT
GQNGTTEEVTSEEEEEEEEMAEDIEDLDHYEMKEEEPISGKKSEDEGIEK
ENLAILEKIRKTQRQDHLNGAVSGSVQASDRLMKELRDIYRSQSYKTGIY
SVELINDSLYDWHVKLQKVDPDSPLHSDLQILKEKEGIEYILLNFSFKDN
FPFDPPFVRVVLPVLSGGYVLGGGALCMELLTKQGWSSAYSIESVIMQIN
ATLVKGKARVQFGANKNQYNLARAQQSYNSIVQIHEKNGWYTPPKEDG

• Vector Type: Bacterial
• Antibiotic: Amp
• Full Snapgene Map: pET15b 6His 3C UBE2Q261.79 KB Snapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU12801
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter 6HIS for bacterial expression.; ; Estimated MW of expressed protein : 45262 Daltons
• Low Temp: No