cDNA Clone USP4

• Plasmid: pET156P USP4
• Parent Plasmid: pET15b 6His 3C
• Expressed: 6His-USP4
• Genbank: NM_003363
• Tag: 6His

Sequence of Insert

GGATCCATGGCGGAAGGTGGAGGCTGCCGTGAGCGACCGGATGCGGAGAC
TCAGAAGTCCGAGCTTGGACCCTTAATGAGGACCACACTCCAACGCGGGG
CGCAGTGGTATCTTATTGACAGCCGGTGGTTCAAGCAGTGGAAGAAGTAT
GTGGGCTTTGACAGCTGGGACATGTACAATGTGGGTGAACATAACCTATT
TCCTGGCCCAATAGACAACTCTGGGCTATTTTCAGATCCTGAGAGTCAGA
CCTTGAAAGAACACTTAATTGATGAATTGGACTATGTATTGGTCCCTACC
GAGGCGTGGAATAAACTACTAAACTGGTACGGCTGTGTAGAAGGCCAGCA
ACCCATCGTCAGAAAAGTTGTGGAGCATGGCCTGTTTGTCAAGCACTGCA
AAGTCGAGGTGTATTTGCTGGAACTGAAGCTCTGTGAGAACAGTGACCCC
ACCAATGTGCTGAGTTGCCATTTCAGCAAGGCAGACACCATTGCAACCAT
CGAGAAAGAGATGCGGAAGCTATTCAACATCCCTGCGGAGCGTGAAACAC
GGCTCTGGAACAAATACATGAGCAACACCTACGAGCAGTTGAGCAAGCTA
GACAACACTGTCCAGGATGCTGGGCTATACCAGGGTCAGGTGCTAGTAAT
TGAGCCTCAAAATGAAGATGGCACATGGCCCAGGCAGACCTTGCAGTCAA
AATCAAGCACTGCGCCTAGCAGAAATTTTACTACCTCTCCAAAATCATCA
GCAAGTCCCTATTCCTCAGTGTCTGCCTCTCTCATTGCAAATGGTGATAG
CACTAGCACCTGTGGGATGCACAGTTCCGGTGTCAGCAGGGGTGGATCTG
GCTTTTCTGCTTCGTATAATTGTCAGGAGCCACCATCCTCTCATATACAA
CCTGGGCTCTGTGGACTTGGAAACCTGGGAAACACCTGCTTCATGAACTC
CGCTTTGCAGTGTTTGAGCAACACTGCACCACTGACTGACTACTTTCTCA
AAGATGAGTATGAAGCCGAAATCAACAGAGACAACCCTCTGGGGATGAAA
GGGGAAATTGCAGAAGCCTATGCTGAACTCATTAAGCAGATGTGGTCTGG
AAGGGACGCCCATGTGGCACCTCGCATGTTCAAAACTCAAGTAGGACGTT
TTGCTCCTCAATTTTCTGGCTACCAGCAACAAGATTCTCAGGAGCTGCTG
GCCTTTCTTCTAGATGGATTGCATGAAGATCTGAACCGGGTAAAGAAAAA
GCCCTACTTGGAGCTGAAGGATGCCAATGGGCGGCCAGATGCGGTGGTGG
CAAAGGAAGCCTGGGAGAATCACAGGTTGAGGAATGATTCTGTGATTGTG
GATACTTTCCATGGCCTCTTCAAATCTACTTTGGTTTGCCCAGAATGTGC
TAAGGTTTCTGTGACCTTTGACCCATTTTGCTATCTAACGCTGCCACTGC
CCTTGAAGAAAGATCGAGTTATGGAGGTTTTCCTGGTTCCTGCTGACCCT
CACTGCAGACCTACTCAGTACCGTGTGACTGTGCCGCTGATGGGGGCTGT
GTCCGACCTGTGCGAGGCTCTCTCCAGGCTGTCTGGCATTGCTGCAGAAA
ATATGGTGGTCGCAGATGTGTATAATCACCGATTCCACAAAATTTTCCAA
ATGGATGAAGGTTTAAACCACATCATGCCTCGGGATGACATTTTCGTGTA
CGAGGTCTGCAGCACTTCCGTGGATGGCTCGGAATGTGTCACGCTTCCAG
TCTACTTCAGGGAGAGGAAGTCCAGGCCATCAAGCACTTCCTCCGCATCA
GCGCTATATGGGCAGCCACTATTGCTTTCTGTCCCCAAGCACAAGTTAAC
CCTTGAGTCTTTGTACCAGGCTGTTTGTGATCGTATCAGCCGCTATGTGA
AACAGCCTTTACCTGATGAGTTTGGCAGCTCACCCTTGGAGCCAGGGGCC
TGCAATGGCTCCAGGAACAGCTGTGAAGGAGAAGATGAGGAAGAAATGGA
GCATCAGGAAGAAGGCAAAGAGCAGCTTTCAGAAACAGAAGGCAGTGGGG
AAGATGAGCCAGGAAATGACCCCAGTGAGACCACCCAAAAGAAGATCAAA
GGCCAGCCCTGCCCAAAAAGGCTTTTTACCTTCAGTCTTGTGAACTCCTA
TGGAACAGCTGACATAAATTCACTTGCAGCTGATGGAAAACTACTTAAAC
TCAACTCTCGATCTACACTGGCCATGGATTGGGACAGTGAAACTCGGAGA
CTTTACTATGATGAGCAAGAATCTGAGGCCTACGAGAAGCATGTGAGCAT
GTTGCAGCCTCAGAAGAAGAAGAAGACCACAGTGGCCCTGAGAGACTGCA
TCGAGCTCTTCACCACCATGGAGACCCTTGGGGAGCATGACCCCTGGTAC
TGTCCCAACTGTAAGAAGCATCAACAGGCCACAAAAAAGTTTGACCTATG
GTCCTTGCCCAAGATCCTGGTGGTCCACCTCAAACGTTTCTCCTACAACA
GATACTGGAGGGATAAGCTCGACACAGTCGTAGAATTCCCAATCAGAGGG
CTGAACATGTCCGAGTTTGTCTGTAACCTGTCAGCAAGGCCTTATGTGTA
CGACCTCATTGCCGTGTCCAATCATTATGGAGCCATGGGGGTTGGCCACT
ACACTGCATATGCGAAGAACAAACTGAATGGTAAATGGTATTACTTTGAT
GATAGCAACGTGTCCCTGGCCTCTGAGGATCAGATAGTGACTAAAGCAGC
TTATGTGCTATTTTACCAACGTCGAGATGATGAATTTTATAAGACACCTT
CACTTAGCAGTTCTGGTTCCTCTGATGGAGGGACACGACCAAGCAGCTCT
CAGCAGGGCTTTGGGGATGATGAGGCTTGCAGCATGGACACCAACTAAGC
GGCCGC

Amino Acid Sequence

MGSSHHHHHHSSGLEVLFQGPGSMAEGGGCRERPDAETQKSELGPLMRTT
LQRGAQWYLIDSRWFKQWKKYVGFDSWDMYNVGEHNLFPGPIDNSGLFSD
PESQTLKEHLIDELDYVLVPTEAWNKLLNWYGCVEGQQPIVRKVVEHGLF
VKHCKVEVYLLELKLCENSDPTNVLSCHFSKADTIATIEKEMRKLFNIPA
ERETRLWNKYMSNTYEQLSKLDNTVQDAGLYQGQVLVIEPQNEDGTWPRQ
TLQSKSSTAPSRNFTTSPKSSASPYSSVSASLIANGDSTSTCGMHSSGVS
RGGSGFSASYNCQEPPSSHIQPGLCGLGNLGNTCFMNSALQCLSNTAPLT
DYFLKDEYEAEINRDNPLGMKGEIAEAYAELIKQMWSGRDAHVAPRMFKT
QVGRFAPQFSGYQQQDSQELLAFLLDGLHEDLNRVKKKPYLELKDANGRP
DAVVAKEAWENHRLRNDSVIVDTFHGLFKSTLVCPECAKVSVTFDPFCYL
TLPLPLKKDRVMEVFLVPADPHCRPTQYRVTVPLMGAVSDLCEALSRLSG
IAAENMVVADVYNHRFHKIFQMDEGLNHIMPRDDIFVYEVCSTSVDGSEC
VTLPVYFRERKSRPSSTSSASALYGQPLLLSVPKHKLTLESLYQAVCDRI
SRYVKQPLPDEFGSSPLEPGACNGSRNSCEGEDEEEMEHQEEGKEQLSET
EGSGEDEPGNDPSETTQKKIKGQPCPKRLFTFSLVNSYGTADINSLAADG
KLLKLNSRSTLAMDWDSETRRLYYDEQESEAYEKHVSMLQPQKKKKTTVA
LRDCIELFTTMETLGEHDPWYCPNCKKHQQATKKFDLWSLPKILVVHLKR
FSYNRYWRDKLDTVVEFPIRGLNMSEFVCNLSARPYVYDLIAVSNHYGAM
GVGHYTAYAKNKLNGKWYYFDDSNVSLASEDQIVTKAAYVLFYQRRDDEF
YKTPSLSSSGSSDGGTRPSSSQQGFGDDEACSMDTN*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU14350
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter 6HIS for bacterial expression.; ; Not available - use DU23032 instead.; ; Estimated MW of expressed protein : 111006 Daltons