cDNA Clone ATG4B

• Synonyms: APG4B, AUTL1, MGC1353
• Plasmid: pBabe.puro-DU-GFP ATG4B
• Parent Plasmid: pBabeD puro GFP
• Expressed: GFP ATG4B
• Genbank: NM_013325.4 GI:47132610*
• Tag: GFP
• Species: Human

Sequence of Insert

GGATCCATGGACGCAGCTACTCTGACCTACGACACTCTCCGGTTTGCTGA
GTTTGAAGATTTTCCTGAGACCTCAGAGCCCGTTTGGATACTGGGTAGAA
AATACAGCATTTTCACAGAAAAGGACGAGATCTTGTCTGATGTGGCATCT
AGACTTTGGTTTACATACAGGAAAAACTTTCCAGCCATTGGGGGGACAGG
CCCCACCTCGGACACAGGCTGGGGCTGCATGCTGCGGTGTGGACAGATGA
TCTTTGCCCAAGCCCTGGTGTGCCGGCACCTAGGCCGAGATTGGAGGTGG
ACACAAAGGAAGAGGCAGCCAGACAGCTACTTCAGCGTCCTCAACGCATT
CATCGACAGGAAGGACAGTTACTACTCCATTCACCAGATAGCGCAAATGG
GAGTTGGCGAAGGCAAGTCCATAGGCCAGTGGTACGGGCCCAACACTGTC
GCCCAGGTCCTGAAGAAGCTTGCTGTCTTCGATACGTGGAGCTCCTTGGC
GGTCCACATTGCAATGGACAACACTGTTGTGATGGAGGAAATCAGAAGGT
TGTGCAGGACCAGCGTTCCCTGTGCAGGCGCCACTGCGTTTCCTGCAGAT
TCCGACCGGCACTGCAACGGATTCCCTGCCGGAGCTGAGGTCACCAACAG
GCCGTCGCCATGGAGACCCCTGGTACTTCTCATTCCCCTGCGCCTGGGGC
TCACGGACATCAACGAGGCCTACGTGGAGACGCTGAAGCACTGCTTCATG
ATGCCCCAGTCCCTGGGCGTCATCGGAGGGAAGCCCAACAGCGCCCACTA
CTTCATCGGCTACGTTGGTGAGGAGCTCATCTACCTGGACCCCCACACCA
CGCAGCCAGCCGTGGAGCCCACTGATGGCTGCTTCATCCCGGACGAGAGC
TTCCACTGCCAGCACCCGCCGTGCCGCATGAGCATCGCGGAGCTTGACCC
GTCCATCGCTGTGGGGTTTTTCTGTAAGACTGAAGATGACTTCAATGATT
GGTGCCAGCAAGTCAAAAAGCTGTCTCTGCTCGGAGGTGCCCTGCCCATG
TTTGAGCTGGTGGAGCAGCAGCCTTCACATCTGGCCTGCCCCGACGTCCT
GAACCTGTCCCTAGATTCTTCTGATGTAGAGCGACTGGAAAGATTCTTCG
ACTCAGAAGATGAAGACTTTGAAATCCTGTCCCTTTGAGCGGCCGC

Amino Acid Sequence

MHVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFIC
TTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTI
FFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSH
NVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDN
HYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSMDAAT
LTYDTLRFAEFEDFPETSEPVWILGRKYSIFTEKDEILSDVASRLWFTYR
KNFPAIGGTGPTSDTGWGCMLRCGQMIFAQALVCRHLGRDWRWTQRKRQP
DSYFSVLNAFIDRKDSYYSIHQIAQMGVGEGKSIGQWYGPNTVAQVLKKL
AVFDTWSSLAVHIAMDNTVVMEEIRRLCRTSVPCAGATAFPADSDRHCNG
FPAGAEVTNRPSPWRPLVLLIPLRLGLTDINEAYVETLKHCFMMPQSLGV
IGGKPNSAHYFIGYVGEELIYLDPHTTQPAVEPTDGCFIPDESFHCQHPP
CRMSIAELDPSIAVGFFCKTEDDFNDWCQQVKKLSLLGGALPMFELVEQQ
PSHLACPDVLNLSLDSSDVERLERFFDSEDEDFEILSL*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU36770
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: *Sequence differences compared with NM_013325.4 CDS:; t1026c (silent); t1061a (L354Q) – present in many published sequences for ATG4B; Retrovirus vector providing the viral package signal, transcription and processing elements and target gene. For expression in mammalian cells.