cDNA Clone USP49

• Plasmid: pFBHTb USP49
• Parent Plasmid: pFastBac HTb
• Expressed: 6His-USP49
• Genbank: NM_018561.3 GI:34147686
• Tag: 6His
• Species: Human

Sequence of Insert

AGATCTATGGATAGATGCAAACATGTAGGGCGGTTACGGCTCGCCCAGGA
CCACTCCATCCTGAACCCTCAGAAGTGGTGCTGCTTAGAGTGTGCCACCA
CCGAGTCCGTGTGGGCCTGCCTCAAGTGCTCCCACGTGGCCTGCGGCCGC
TATATTGAGGACCACGCCCTGAAACACTTTGAGGAGACGGGACACCCGCT
AGCCATGGAAGTCCGGGATCTCTACGTGTTCTGTTACCTGTGCAAGGACT
ACGTGCTCAATGATAACCCAGAGGGGGACCTGAAGCTGCTAAGAAGCTCC
CTCCTGGCGGTCCGGGGCCAGAAACAGGACACGCCGGTGAGACGTGGGCG
GACGCTGCGGTCCATGGCTTCGGGTGAGGACGTGGTCCTGCCGCAGCGCG
CTCCTCAGGGACAGCCGCAGATGCTCACGGCTCTGTGGTACCGGCGTCAG
CGCCTGCTGGCCAGGACGCTGCGGCTGTGGTTCGAGAAGAGCTCCCGGGG
CCAGGCGAAGCTGGAGCAGCGGCGGCAGGAGGAGGCCCTGGAGCGCAAGA
AGGAGGAGGCGCGGAGGCGGCGGCGCGAGGTGAAACGGCGGCTGCTGGAG
GAGCTGGCCAGCACCCCTCCGCGCAAGAGTGCACGGCTGCTCCTGCACAC
GCCCCGCGACGCGGGCCCGGCTGCCTCGCGCCCCGCCGCCCTCCCTACCT
CACGCAGAGTGCCCGCCGCCACACTCAAGCTGCGTCGCCAGCCGGCCATG
GCCCCAGGCGTCACGGGCCTGCGCAACCTGGGCAACACCTGCTACATGAA
CTCCATCCTCCAGGTGCTCAGCCACCTCCAGAAGTTCCGAGAATGTTTCC
TCAACCTTGACCCTTCCAAAACGGAACATCTGTTTCCCAAAGCCACCAAC
GGGAAGACTCAGCTTTCTGGCAAGCCAACCAACAGCTCGGCCACGGAGCT
GTCCTTGAGAAATGACAGGGCCGAGGCATGCGAGCGGGAGGGCTTCTGCT
GGAACGGCAGGGCCTCCATTAGTCGGAGTCTGGAGCTCATCCAGAACAAG
GAGCCGAGTTCAAAGCACATTTCCCTCTGCCGTGAACTGCACACCCTCTT
CCGAGTCATGTGGTCCGGGAAGTGGGCCCTAGTGTCGCCCTTCGCCATGC
TGCACTCAGTGTGGAGCCTGATCCCTGCCTTCCGCGGCTACGACCAACAG
GACGCGCAGGAATTTCTCTGCGAGCTGCTGCACAAGGTGCAGCAGGAACT
CGAGTCTGAGGGCACCACACGCCGGATCCTCATCCCCTTCTCCCAGAGGA
AGCTCACCAAACAGGTCTTAAAGGTGGTGAATACCATATTTCATGGGCAG
CTGCTCAGTCAGGTCACATGTATATCATGCAATTACAAATCCAATACCAT
TGAGCCCTTTTGGGACCTATCCCTGGAATTCCCTGAACGCTATCACTGCA
TAGAAAAGGGGTTTGTCCCTTTGAATCAAACAGAGTGCTTGCTCACTGAG
ATGCTGGCCAAATTCACAGAGACAGAGGCCCTGGAAGGGAGAATCTACGC
TTGTGACCAGTGTAACAGCAAACGACGAAAATCCAATCCCAAACCCCTTG
TTCTGAGTGAAGCTAGAAAGCAGTTAATGATCTACAGACTACCTCAGGTT
CTCCGGCTGCACCTTAAAAGATTCAGGTGGTCTGGCCGTAATCATCGAGA
GAAGATTGGGGTCCATGTCGTCTTTGACCAGGTATTAACCATGGAACCTT
ACTGCTGCAGGGACATGCTCTCCTCTCTTGACAAAGAGACCTTTGCCTAT
GATCTCTCCGCAGTGGTCATGCATCACGGGAAAGGGTTTGGCTCAGGACA
CTACACAGCCTATTGCTACAACACAGAGGGAGGTGCGTGCGCTTTACTCT
GTGGGGTGGGGGACACGGAAAGGGGTTGAGATCT

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMGSMDRCKHVGRLRLAQDHSILNPQ
KWCCLECATTESVWACLKCSHVACGRYIEDHALKHFEETGHPLAMEVRDL
YVFCYLCKDYVLNDNPEGDLKLLRSSLLAVRGQKQDTPVRRGRTLRSMAS
GEDVVLPQRAPQGQPQMLTALWYRRQRLLARTLRLWFEKSSRGQAKLEQR
RQEEALERKKEEARRRRREVKRRLLEELASTPPRKSARLLLHTPRDAGPA
ASRPAALPTSRRVPAATLKLRRQPAMAPGVTGLRNLGNTCYMNSILQVLS
HLQKFRECFLNLDPSKTEHLFPKATNGKTQLSGKPTNSSATELSLRNDRA
EACEREGFCWNGRASISRSLELIQNKEPSSKHISLCRELHTLFRVMWSGK
WALVSPFAMLHSVWSLIPAFRGYDQQDAQEFLCELLHKVQQELESEGTTR
RILIPFSQRKLTKQVLKVVNTIFHGQLLSQVTCISCNYKSNTIEPFWDLS
LEFPERYHCIEKGFVPLNQTECLLTEMLAKFTETEALEGRIYACDQCNSK
RRKSNPKPLVLSEARKQLMIYRLPQVLRLHLKRFRWSGRNHREKIGVHVV
FDQVLTMEPYCCRDMLSSLDKETFAYDLSAVVMHHGKGFGSGHYTAYCYN
TEGGACALLCGVGDTERG*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU36246
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Baculovirus expression plasmid with N terminal 6His tag; ; Estimated MW of expressed protein : 76824 Daltons