cDNA Clone ZNRF2

• Plasmid: pGEX6P-ZNRF2
• Parent Plasmid: pGEX6P1
• Expressed: GST-ZNRF2
• Genbank: NM_147128.3 GI:40316919
• Tag: GST
• Species: Human

Sequence of Insert

ggatccatgggcgccaaacagagcggcccggccgccgctaacggccgcacgcgcgcgtactcgggctcggatctaccttccagtagcagcggaggcgccaatgggaccgcgggcggcggcgggggcgctcgggccgccgccgcggggaggttcccggctcaggtgcccagcgcgcaccagcccagcgcctccggcggcgccgcggcggccgcggcggccccggcagccccggcggccccgcgcagccgctccctcggcggggccgtggggagcgtggcgtcgggggcccgcgcggcgcagtccccc
ttcagcatcccgaacagcagcagcggcccgtacggctcgcaggactcggt
gcacagcagccctgaggacggcggcggcggccgggaccggccggtgggcg
ggagccccggcgggccgcgcctggtgatcggctccttaccagctcacctc
tcgccgcacatgtttggaggatttaagtgccctgtatgctcaaaatttgt
atcctcagatgaaatggatttgcatcttgtaatgtgtttaacaaagccac
gaataacctataatgaggatgtactgagtaaagatgctggggaatgtgca
atatgccttgaagaattgcagcagggagatactatagcacgactgccttg
tctatgcatatatcataaaggctgcatagatgaatggtttgaagtaaata
gatcttgccctgagcacccttcagattaagaattc

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMGAKQSGPAAANGRTRAYS
GSDLPSSSSGGANGTAGGGGGARAAAAGRFPAQVPSAHQPSASGGAAAAA
AAPAAPAAPRSRSLGGAVGSVASGARAAQSPFSIPNSSSGPYGSQDSVHS
SPEDGGGGRDRPVGGSPGGPRLVIGSLPAHLSPHMFGGFKCPVCSKFVSS
DEMDLHLVMCLTKPRITYNEDVLSKDAGECAICLEELQQGDTIARLPCLC
IYHKGCIDEWFEVNRSCPEHPSD*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU16232
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express GST N terminal fusion.; ; Estimated MW of expressed protein : 50936 Daltons; ; All ZNRF2 plasmids MUST be grown at 30 degrees C or less to prevent recombination.