cDNA Clone DCUN1D1

• Plasmid: pTRE-Tight-maxFPGreen-SV5-DCUN1D1 D211A,A235R,D241A
• Parent Plasmid: pTRE-Tight
• Expressed: maxFPGreen-SV5-DCUN1D1 D211A,A235R,D241A
• Genbank: NM_020640*
• Tag: SV5
• Species: Human

Sequence of Insert

GCTAGCGTTTAAACTTAAGCTTGCCACCatggagagcgacgagagcggcctgcccgccatggagatcgagtgccgcatcaccggcaccctgaacggcgtggagttcgagctggtgggcggcggagagggcacccccgagcagggccgcatgaccaacaagatgaagagcaccaaaggcgccctgaccttcagcccctacctgctgagccacgtgatgggctacggcttctaccacttcggcacctaccccagcggctacgagaaccccttcctgcacgccatcaacaacggcggctacaccaacacccgcatcgagaagtacgaggacggcggcgtgctgcacgtgagcttcagctaccgctacgaggccggccgcgtgatcggcgacttcaaggtgatgggcaccggcttccccgaggacagcgtgatcttcaccgacaagatcatccgcagcaacgccaccgtggagcacctgcaccccatgggcgataacgatctggatggcagcttcacccgcaccttcagcctgcgcgacggcggctactacagctccgtggtggacagccacatgcacttcaagagcgccatccaccccagcatcctgcagaacgggggccccatgttcgccttccgccgcgtggaggaggatcacagcaacaccgagctgggcatcgtggagtaccagcacgccttcaagaccccggatgcagatgccggtgaagaaggtaccGAGCTCggatccGGCAAGCCCATCCCCAACCCCCTGCTGGGCCTGGACAGCACCATGAACAAGTTGAAATCATCGCAGAAGGATAAAGTTCGTCAGTTTATGATCTTCACACAATCTAGTGAAAAAACAGCAGTAAGTTGTCTTTCTCAAAATGACTGGAAGTTAGATGTTGCAACAGATAATTTTTTCCAAAATCCTGAACTTTATATACGAGAGAGTGTAAAAGGATCATTGGACAGGAAGAAGTTAGAACAGCTGTACAATAGATACAAAGACCCTCAAGATGAGAATAAAATTGGAATAGATGGCATACAGCAGTTCTGTGATGACCTGGCACTCGATCCAGCCAGCATTAGTGTGTTGATTATTGCGTGGAAGTTCAGAGCAGCAACACAGTGCGAGTTCTCCAAACAGGAGTTCATGGATGGCATGACAGAATTAGGATGTGACAGCATAGAAAAACTAAAGGCCCAGATACCCAAGATGGAACAAGAATTGAAAGAACCAGGACGATTTAAGGATTTTTACCAGTTTACTTTTAATTTTGCAAAGAATCCAGGACAAAAAGGATTAGATCTAGAAATGGCCATTGCCTACTGGAACTTAGTGCTTAATGGAAGATTTAAATTCTTAGACTTATGGAATAAATTTTTGTTGGAACATCATAAACGATCAATACCAAAAGCCACTTGGAATCTTCTTTTAGACTTCAGTACGATGATTGCAGATGACATGTCTAATTATGATGAAGAAGGAAGATGGCCTGTTCTTATTGCTGACTTTGTGGAATTTGCACGCCCTCAAATTGCTGGGACAAAAAGTACAACAGTGTAGGCGGCCGC

Amino Acid Sequence

MESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKG
ALTFSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYED
GGVLHVSFSYRYEAGRVIGDFKVMGTGFPEDSVIFTDKIIRSNATVEHLH
PMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
FRRVEEDHSNTELGIVEYQHAFKTPDADAGEEGTELGSGKPIPNPLLGLD
STMNKLKSSQKDKVRQFMIFTQSSEKTAVSCLSQNDWKLDVATDNFFQNP
ELYIRESVKGSLDRKKLEQLYNRYKDPQDENKIGIDGIQQFCDDLALDPA
SISVLIIAWKFRAATQCEFSKQEFMDGMTELGCDSIEKLKAQIPKMEQEL
KEPGRFKDFYQFTFNFAKNPGQKGLDLEMAIAYWNLVLNGRFKFLDLWNK
FLLEHHKRSIPKATWNLLLDFSTMIADDMSNYDEEGRWPVLIADFVEFAR
PQIAGTKSTTV*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU20606
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: *carries g318a mutation (silent Ala106) and the following substitutions:; DCUN1D1 a632c (confers D211A), g703a/c704g (confers A235R) and a722c (confers D241A).; Mammalian response plasmid for expression of maxFPGreen-SV5-DCUN1D1 D211A,A235R,D241A (for use with Clontech Advanced Tet-ON system); ; Estimated MW of expressed protein : 57746 Daltons