cDNA Clone SrtA (sortase)

• Plasmid: pET28a-S-tag-SrtA 60-206
• Parent Plasmid: pET28a original
• Expressed: 6His-S-tag-SrtA 60-206
• Genbank: ADC38675
• Tag: 6His
• Species: S. aureus

Sequence of Insert

CATATGAAAGAAACCGCTGCTGCTAAATTCGAACGCCAGCACATGGAC
AGCCAAGCTAAACCTCAAATTCCGAAAGATAAATCAAAAGTGGCAGGCTA
TATTGAAATTCCAGATGCTGATATTAAAGAACCAGTATATCCAGGACCAG
CAACACCTGAACAATTAAATAGAGGTGTAAGCTTTGCAGAAGAAAATGAA
TCACTAGATGATCAAAATATTTCAATTGCAGGACACACTTTCATTGACCG
TCCGAACTATCAATTTACAAATCTTAAAGCAGCCAAAAAAGGTAGTATGG
TGTACTTTAAAGTTGGTAATGAAACACGTAAGTATAAAATGACAAGTATA
AGAGATGTTAAGCCAACAGATGTAGAAGTTCTAGATGAACAAAAAGGTAA
AGATAAACAATTAACATTAATTACTTGTGATGATTACAATGAAAAGACAG
GCGTTTGGGAAAAACGTAAAATCTTTGTAGCTACAGAAGTCAAATAAGGA
TCC

Amino Acid Sequence

MGSSHHHHHHSSGLVPRGSHMKETAAAKFERQHMDSQAKPQIPKDKSKVA
GYIEIPDADIKEPVYPGPATPEQLNRGVSFAEENESLDDQNISIAGHTFI
DRPNYQFTNLKAAKKGSMVYFKVGNETRKYKMTSIRDVKPTDVEVLDEQK
GKDKQLTLITCDDYNEKTGVWEKRKIFVATEVK*

• Vector Type: Bacterial
• Antibiotic: Kan
• DU Number: DU20983
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression plasmid for 6HIS- S-tag-SrtA aa 60-206; ; Estimated MW of expressed protein : 20694 Daltons