cDNA Clone Fan1

• Plasmid: pGEX6P-1-Fan1 UBZ domain dimer (no start or stop)
• Parent Plasmid: pGEX6P1
• Expressed: GST-C3-Fan1 UBZ domain dimer (no start or stop)
• Genbank: NM_014967.3 GI:62198230
• Tag: GST
• Species: Human

Sequence of Insert

GCCGGCAAACTTGCCTGCCCCGTTTGCAGTAAAATGGTGCCTAG
ATATGACTTAAACCGGCACCTTGATGAAATGTGTGCTAACAATGACTTCG
GTGGAGGTGGAGGTGGAGGCGCC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSAGKLACPVCSKMVPRYDLN
RHLDEMCANNDFGGGGGGGGKLACPVCSKMVPRYDLNRHLDEMCANNDFG
GGGGGGAAA

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU20728
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Intermediate for construction of UBZ domain non-stop concatamers.; ; All MTMR15 (FAN1) plasmids MUST be grown at 30 degrees C or less to prevent recombination.; parental SC20708 (SC20103 based -pGEX6P-1 minus Sfo1 site)