cDNA Clone DCUN1D1

• Plasmid: pTRE-Tight-SV5-DCUN1D1
• Parent Plasmid: pTRE-Tight
• Expressed: SV5-DCUN1D1
• Genbank: NM_020640*
• Tag: SV5
• Species: Human

Sequence of Insert

GGATCCGCCACCATGGGCAAGCCCATCCCCAACCCCCTGCTGGGCCTGGA
CAGCACCATGAACAAGTTGAAATCATCGCAGAAGGATAAAGTTCGTCAGT
TTATGATCTTCACACAATCTAGTGAAAAAACAGCAGTAAGTTGTCTTTCT
CAAAATGACTGGAAGTTAGATGTTGCAACAGATAATTTTTTCCAAAATCC
TGAACTTTATATACGAGAGAGTGTAAAAGGATCATTGGACAGGAAGAAGT
TAGAACAGCTGTACAATAGATACAAAGACCCTCAAGATGAGAATAAAATT
GGAATAGATGGCATACAGCAGTTCTGTGATGACCTGGCACTCGATCCAGC
CAGCATTAGTGTGTTGATTATTGCGTGGAAGTTCAGAGCAGCAACACAGT
GCGAGTTCTCCAAACAGGAGTTCATGGATGGCATGACAGAATTAGGATGT
GACAGCATAGAAAAACTAAAGGCCCAGATACCCAAGATGGAACAAGAATT
GAAAGAACCAGGACGATTTAAGGATTTTTACCAGTTTACTTTTAATTTTG
CAAAGAATCCAGGACAAAAAGGATTAGATCTAGAAATGGCCATTGCCTAC
TGGAACTTAGTGCTTAATGGAAGATTTAAATTCTTAGACTTATGGAATAA
ATTTTTGTTGGAACATCATAAACGATCAATACCAAAAGACACTTGGAATC
TTCTTTTAGACTTCAGTACGATGATTGCAGATGACATGTCTAATTATGAT
GAAGAAGGAGCATGGCCTGTTCTTATTGATGACTTTGTGGAATTTGCACG
CCCTCAAATTGCTGGGACAAAAAGTACAACAGTGTAGGCGGCCGC

Amino Acid Sequence

MGKPIPNPLLGLDSTMNKLKSSQKDKVRQFMIFTQSSEKTAVSCLSQNDW
KLDVATDNFFQNPELYIRESVKGSLDRKKLEQLYNRYKDPQDENKIGIDG
IQQFCDDLALDPASISVLIIAWKFRAATQCEFSKQEFMDGMTELGCDSIE
KLKAQIPKMEQELKEPGRFKDFYQFTFNFAKNPGQKGLDLEMAIAYWNLV
LNGRFKFLDLWNKFLLEHHKRSIPKDTWNLLLDFSTMIADDMSNYDEEGA
WPVLIDDFVEFARPQIAGTKSTTV*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU20605
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: *carries g318a mutation (silent Ala106) ; Mammalian response plasmid for expression of SV5-DCUN1D1 wild type (for use with Clontech Advanced Tet-ON system); ; Estimated MW of expressed protein : 30277 Daltons