cDNA Clone NEDD8

• Plasmid: pCMV5 HA NEDD8 (mature form truncated 1-74)
• Parent Plasmid: pCMV5 HA1
• Expressed: HA-NEDD8 (mature form 1-74)
• Genbank: Q15843
• Tag: HA
• Species: Human

Sequence of Insert

GGATCCATGCTAATTAAAGTGAAGACGCTGACCGGAAAGGAGATTGAGAT
TGACATTGAACCTACAGACAAGGTGGAGCGAATCAAGGAGCGTGTGGAGG
AGAAAGAGGGAATCCCCCCACAACAGCAGAGGCTCATCTACAGTGGCAAG
CAGATGAATGATGAGAAGACAGCAGCTGATTACAAGATTTTAGGTGGTTC
AGTCCTTCACCTGGTGTTGGCTCTGAGATGAGCGGCCGC

Amino Acid Sequence

MYPYDVPDYAGSMLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPP
QQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALR*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU20489
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N-terminally HA tagged NEDD8 mature form (residues 1- 74) for mammalian expression; ; Estimated MW of expressed protein : 9805 Daltons