cDNA Clone DCUN1D3

• Plasmid: pEBG6P-DCUN1D3
• Parent Plasmid: pEBG6P
• Expressed: GST-DCUN1D3
• Genbank: NM_173475*
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGGGCCAGTGTGTCACCAAGTGTAAGAATCCCTCATCGACCCT
GGGCAGCAAAAATGGAGACCGTGAGCCCAGCAACAAGTCACATAGCAGGA
GGGGTGCAGGCCACCGTGAGGAGCAGGTACCACCCTGTGGCAAGCCAGGT
GGAGATATCCTCGTCAACGGGACCAAGAAGGCCGAGGCTGCCACTGAGGC
CTGCCAGCTGCCAACGTCCTCGGGAGATGCTGGGAGGGAGTCCAAGTCCA
ATGCCGAGGAGTCTTCCTTGCAAAGATTGGAAGAACTGTTCAGGCGCTAC
AAGGATGAGCGGGAAGATGCAATTTTGGAGGAAGGCATGGAGCGCTTTTG
CAATGACCTGTGTGTTGACCCCACAGAATTTCGAGTGCTGCTCTTGGCTT
GGAAGTTCCAGGCTGCAACCATGTGCAAATTCACCAGGAAGGAGTTTTTT
GATGGCTGCAAAGCAATAAGTGCAGACAGCATTGACGGAATCTGTGCACG
GTTCCCTAGCCTCTTAACAGAAGCCAAACAAGAGGATAAATTCAAGGATC
TCTACCGGTTTACATTTCAGTTTGGCCTGGACTCTGAAGAAGGGCAGCGG
TCACTGCATCGGGAAATAGCCATTGCCCTGTGGAAACTAGTCTTTACCCA
GAACAATCCTCCGGTATTGGACCAATGGCTAAACTTCCTAACAGAGAACC
CCTCGGGGATCAAGGGCATCTCCCGGGACACTTGGAACATGTTCCTTAAC
TTCACTCAGGTGATTGGCCCTGACCTCAGCAACTACAGTGAAGATGAGGC
CTGGCCAAGTCTCTTTGACACCTTTGTGGAGTGGGAAATGGAGCGAAGGA
AAAGAGAAGGGGAAGGGAGAGGTGCACTCAGCTCAGGGCCTGAGGGCTTG
TGTCCCGAGGAGCAGACTTAGGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSMGQCVTKCKNPSS
TLGSKNGDREPSNKSHSRRGAGHREEQVPPCGKPGGDILVNGTKKAEAAT
EACQLPTSSGDAGRESKSNAEESSLQRLEELFRRYKDEREDAILEEGMER
FCNDLCVDPTEFRVLLLAWKFQAATMCKFTRKEFFDGCKAISADSIDGIC
ARFPSLLTEAKQEDKFKDLYRFTFQFGLDSEEGQRSLHREIAIALWKLVF
TQNNPPVLDQWLNFLTENPSGIKGISRDTWNMFLNFTQVIGPDLSNYSED
EAWPSLFDTFVEWEMERRKREGEGRGALSSGPEGLCPEEQT*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU20453
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: *carries a94c mutation (silent Arg32); Mammalian expression plasmid to express DCUN1D3 with N-terminal GST (contains precission protease site); ; Estimated MW of expressed protein : 61726 Daltons