cDNA Clone Ubiquitin

• Plasmid: pSC-B-Ubiquitin G75A G76A trimer
• Parent Plasmid: pSC-B
• Genbank: P62988*
• Species: Human

Sequence of Insert

GGCgccATGCAGATCTTCGTGAAGACCCTGACTGGTAAGACCATCACTCT
CGAAGTGGAGCCGAGTGACACCATTGAGAATGTCAAGGCAAAGATCCAAG
ACAAGGAAGGCATCCCTCCTGACCAGCAGAGGTTGATCTTTGCTGGGAAA
CAGCTGGAAGATGGACGCACCCTGTCTGACTACAACATCCAGAAAGAGTC
CACCCTGCACCTGGTCCTCCGTCTCAGAgccgccATGCAGATCTTCGTGA
AGACCCTGACTGGTAAGACCATCACTCTCGAAGTGGAGCCGAGTGACACC
ATTGAGAATGTCAAGGCAAAGATCCAAGACAAGGAAGGCATCCCTCCTGA
CCAGCAGAGGTTGATCTTTGCTGGGAAACAGCTGGAAGATGGACGCACCC
TGTCTGACTACAACATCCAGAAAGAGTCCACCCTGCACCTGGTCCTCCGT
CTCAGAgccgccATGCAGATCTTCGTGAAGACCCTGACTGGTAAGACCAT
CACTCTCGAAGTGGAGCCGAGTGACACCATTGAGAATGTCAAGGCAAAGA
TCCAAGACAAGGAAGGCATCCCTCCTGACCAGCAGAGGTTGATCTTTGCT
GGGAAACAGCTGGAAGATGGACGCACCCTGTCTGACTACAACATCCAGAA
AGAGTCCACCCTGCACCTGGTCCTCCGTCTCAGAgccGGC

Amino Acid Sequence

MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQ
LEDGRTLSDYNIQKESTLHLVLRLRAAMQIFVKTLTGKTITLEVEPSDTI
ENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRL
RAAMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAG
KQLEDGRTLSDYNIQKESTLHLVLRLRA

• Vector Type: Other
• Antibiotic: Amp
• DU Number: DU20123
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Vector harbouring trimeric SfoI – NaeI ubiquitin unit (with flanking alanines and no STOP codon) for progressive assembly of polyubiquitin chains.; Differs from reference sequence: C-terminal single Ala instead of GG. N-terminal single Ala.