cDNA Clone Mdm2

• Synonyms: ACTFS; hdm2; HDMX; LSKB
• Plasmid: pGEX6P-1 MDM2 M7-end
• Parent Plasmid: pGEX6P1
• Expressed: GST MDM2 M7-end
• Genbank: NM_002392.6
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGTGCAATACCAACATGTCTGTACCTACTGATGGTGCTGTAAC
CACCTCACAGATTCCAGCTTCGGAACAAGAGACCCTGGTTAGACCAAAGC
CATTGCTTTTGAAGTTATTAAAGTCTGTTGGTGCACAAAAAGACACTTAT
ACTATGAAAGAGGTTCTTTTTTATCTTGGCCAGTATATTATGACTAAACG
ATTATATGATGAGAAGCAACAACATATTGTATATTGTTCAAATGATCTTC
TAGGAGATTTGTTTGGCGTGCCAAGCTTCTCTGTGAAAGAGCACAGGAAA
ATATATACCATGATCTACAGGAACTTGGTAGTAGTCAATCAGCAGGAATC
ATCGGACTCAGGTACATCTGTGAGTGAGAACAGGTGTCACCTTGAAGGTG
GGAGTGATCAAAAGGACCTTGTACAAGAGCTTCAGGAAGAGAAACCTTCA
TCTTCACATTTGGTTTCTAGACCATCTACCTCATCTAGAAGGAGAGCAAT
TAGTGAGACAGAAGAAAATTCAGATGAATTATCTGGTGAACGACAAAGAA
AACGCCACAAATCTGATAGTATTTCCCTTTCCTTTGATGAAAGCCTGGCT
CTGTGTGTAATAAGGGAGATATGTTGTGAAAGAAGCAGTAGCAGTGAATC
TACAGGGACGCCATCGAATCCGGATCTTGATGCTGGTGTAAGTGAACATT
CAGGTGATTGGTTGGATCAGGATTCAGTTTCAGATCAGTTTAGTGTAGAA
TTTGAAGTTGAATCTCTCGACTCAGAAGATTATAGCCTTAGTGAAGAAGG
ACAAGAACTCTCAGATGAAGATGATGAGGTATATCAAGTTACTGTGTATC
AGGCAGGGGAGAGTGATACAGATTCATTTGAAGAAGATCCTGAAATTTCC
TTAGCTGACTATTGGAAATGCACTTCATGCAATGAAATGAATCCCCCCCT
TCCATCACATTGCAACAGATGTTGGGCCCTTCGTGAGAATTGGCTTCCTG
AAGATAAAGGGAAAGATAAAGGGGAAATCTCTGAGAAAGCCAAACTGGAA
AACTCAACACAAGCTGAAGAGGGCTTTGATGTTCCTGATTGTAAAAAAAC
TATAGTGAATGATTCCAGAGAGTCATGTGTTGAGGAAAATGATGATAAAA
TTACACAAGCTTCACAATCACAAGAAAGTGAAGACTATTCTCAGCCATCA
ACTTCTAGTAGCATTATTTATAGCAGCCAAGAAGATGTGAAAGAGTTTGA
AAGGGAAGAAACCCAAGACAAAGAAGAGAGTGTGGAATCTAGTTTGCCCC
TTAATGCCATTGAACCTTGTGTGATTTGTCAAGGTCGACCTAAAAATGGT
TGCATTGTCCATGGCAAAACAGGACATCTTATGGCCTGCTTTACATGTGC
AAAGAAGCTAAAGAAAAGGAATAAGCCCTGCCCAGTATGTAGACAACCAA
TTCAAATGATTGTGCTAACTTATTTCCCCTAGGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMCNTNMSVPTDGAVTTSQI
PASEQETLVRPKPLLLKLLKSVGAQKDTYTMKEVLFYLGQYIMTKRLYDE
KQQHIVYCSNDLLGDLFGVPSFSVKEHRKIYTMIYRNLVVVNQQESSDSG
TSVSENRCHLEGGSDQKDLVQELQEEKPSSSHLVSRPSTSSRRRAISETE
ENSDELSGERQRKRHKSDSISLSFDESLALCVIREICCERSSSSESTGTP
SNPDLDAGVSEHSGDWLDQDSVSDQFSVEFEVESLDSEDYSLSEEGQELS
DEDDEVYQVTVYQAGESDTDSFEEDPEISLADYWKCTSCNEMNPPLPSHC
NRCWALRENWLPEDKGKDKGEISEKAKLENSTQAEEGFDVPDCKKTIVND
SRESCVEENDDKITQASQSQESEDYSQPSTSSSIIYSSQEDVKEFEREET
QDKEESVESSLPLNAIEPCVICQGRPKNGCIVHGKTGHLMACFTCAKKLK
KRNKPCPVCRQPIQMIVLTYFP*

• Vector Type: Bacterial
• Antibiotic: Amp
• Full Snapgene Map: pGEX6P-1 MDM2 M7-end134.69 KB Snapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU43570
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Low Temp: No