cDNA Clone Ubiquitin

• Plasmid: pET24a-ubiquitin (c220a) K48R K63R
• Parent Plasmid: pET24a original
• Expressed: untagged Ubiquitin [K48, K63R]
• Genbank: P62988
• Species: Human

Sequence of Insert

CATATGCAGATCTTCGTGAAGACCCTGACTGGTAAGACCATCACTCTCGA
AGTGGAGCCGAGTGACACCATTGAGAATGTCAAGGCAAAGATCCAAGACA
AGGAAGGCATCCCTCCTGACCAGCAGAGGTTGATCTTTGCTGGGAGACAG
CTGGAAGATGGACGCACCCTGTCTGACTACAACATCCAGAGAGAGTCCAC
CCTGCACCTGGTCCTCCGTCTCCGAGGTGGGTGATAAGGATCC

Amino Acid Sequence

MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGRQL
EDGRTLSDYNIQRESTLHLVLRLRGG**

• Vector Type: Bacterial
• Antibiotic: Kan
• Full Snapgene Map: pET24a-ubiquitin (c220a) K48R K63R69.45 KBSnapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU23835
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Arg74 codon AGA mutated to more common CGA
• Low Temp: No