cDNA Clone MUD1

• Plasmid: pET28a 6His TEVb HALO Mud1 aa291-332 trimer
• Parent Plasmid: pET28a 6His TEVb Halo
• Expressed: 6HIS-TEVb-Halo-Mud1 aa 291-332 trimer
• Genbank: Q10256, NP_001018195.1 NM_001018619.1
• Tag: HALO

Sequence of Insert

GGATCCGGCGGAGGCCCCACTGACCCCGGTTTGAACTCCAAAATTGCACAGCTAGTCAGTATGGGATTTGATCCATTAGAGGCTGCGCAGGCGTTAGACGCTGCGAATGGAGATTTAGATGTAGCTGCTTCTTTTCTCCTTGGCGGAGGCAGATCT

Amino Acid Sequence

MGSSHHHHHHENLYFQMAEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSYVWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIHDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVEEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLIGSEIARWLSTLEISGGSGGGPTDPGLNSKIAQLVSMGFDPLEAAQALDAANGDLDVAASFLLGGGRSGGGPTDPGLNSKIAQLVSMGFDPLEAAQALDAANGDLDVAASFLLGGGRSPTDPGLNSKIAQLVSMGFDPLEAAQALDAANGDLDVAASFLLZAA

• Vector Type: Bacterial
• Antibiotic: Kan
• DU Number: DU23827
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: from Schizosaccharomyces pombe. 22723 ligated twice to 22720 vector to give the trimer. Grown at 30 degrees; Parental Plasmid DU23713