cDNA Clone
MUD1

Plasmid
pET28a-6His_TEV-HALO-Mud1 aa291-332 tetramer
Parent Plasmid
pET28a 6His TEV Halo
Expressed
6HIS-TEV-Halo-Mud1 aa 291-332 tetramer
Genbank
Q10256, NP_001018195.1 NM_001018619.1
Tag
6His
HALO
Sequence of Insert
GGATCCGGCGGAGGCCCCACTGACCCCGGTTTGAACTCCAAAATTGCACAGCTAGTCAGTATGGGATTTGATCCATTAGAGGCTGCGCAGGCGTTAGACGCTGCGAATGGAGATTTAGATGTAGCTGCTTCTTTTCTCCTTGGCGGAGGCAGATCT
Amino Acid Sequence
MGSSHHHHHHENLYFQMAEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTP
VLFLHGNPTSSYVWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDH
VRFMDAFIEALGLEEVVLVIHDWGSALGFHWAKRNPERVKGIAFMEFIRP
IPTWDEWPEFARETFQAFRTTDVGRKLIIDQNVFIEGTLPMGVVRPLTEV
EMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVEEYMDWLHQSPV
PKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLIGS
EIARWLSTLEISGGSGGGPTDPGLNSKIAQLVSMGFDPLEAAQALDAANG
DLDVAASFLLGGGRSGGGPTDPGLNSKIAQLVSMGFDPLEAAQALDAANG
DLDVAASFLLGGGRSGGGPTDPGLNSKIAQLVSMGFDPLEAAQALDAANG
DLDVAASFLLGGGRSPTDPGLNSKIAQLVSMGFDPLEAAQALDAANGDLD
VAASFLL*
Vector Type
Bacterial
Antibiotic
Kan
DU Number
DU23893
Unit Source
Protein Phosphorylation and Ubiquitylation Unit
Location
PPU
MTA
Dundee
Notes
from Schizosaccharomyces pombe. 22723 ligated to 23827 vector to give the tetramer. Grown at 30 degrees
Price
£125.00
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