cDNA Clone GRWD1

• Synonyms: CDW4; GRWD; RRB1; WDR28
• Plasmid: pGEX GST C3 HALO TEV GRWD1
• Parent Plasmid: pGEX6P1 Halo TEV
• Expressed: GST C3 HALO TEV GRWD1
• Genbank: Q9BQ67
• Tag: GST
• Tag: HALO
• Species: Human

Sequence of Insert

ggatccATGGCGGCGCGCAAGGGTCGGCGtCGCACGTGTGAAACCGGGGAACCCAT
GGAAGCCGAGTCCGGCGACACAAGTTCCGAGGGCCCGGCCCAGGTCTACC
TGCCCGGCCGGGGGCCGCCGCTACGCGAAGGGGAGGAGCTGGTCATGGAC
GAGGAGGCCTATGTGCTCTACCACCGAGCGCAGACTGGCGCCCCCTGTCT
CAGCTTTGACATAGTCCGGGATCACCTGGGAGACAACCGGACAGAGCTTC
CTCTTACACTTTACTTGTGTGCTGGGACCCAGGCTGAGAGCGCCCAGAGC
AACAGACTGATGATGCTTCGGATGCACAATCTGCATGGGACAAAGCCCCC
ACCCTCAGAGGGCAGTGATGAAGAAGAAGAGGAGGAAGATGAAGAGGATG
AAGAAGAGCGGAAACCTCAGCTGGAGCTGGCCATGGTGCCCCACTATGGT
GGCATCAACCGAGTTCGGGTGTCATGGCTGGGTGAAGAGCCTGTGGCTGG
GGTGTGGTCAGAGAAGGGCCAGGTGGAGGTGTTTGCGCTGCGGCGGCTTC
TGCAGGTGGTGGAGGAGCCCCAGGCCCTGGCAGCCTTCCTCCGGGATGAG
CAGGCCCAAATGAAGCCCATCTTCTCCTTCGCTGGACACATGGGCGAGGG
CTTTGCCCTTGACTGGTCCCCCCGGGTGACCGGTCGCCTGCTGACCGGTG
ACTGTCAAAAGAACATCCACCTCTGGACACCTACGGACGGCGGCTCCTGG
CACGTGGACCAGCGGCCATTCGTGGGCCACACACGCTCTGTGGAGGACCT
GCAGTGGTCACCGACTGAGAACACGGTGTTTGCCTCCTGCTCAGCTGACG
CCTCCATCCGCATCTGGGACATCCGGGCAGCCCCCAGCAAGGCCTGCATG
CTCACCACAGCCACCGCCCATGATGGGGACGTCAATGTCATCAGCTGGAG
CCGCCGGGAGCCCTTCCTGCTCAGTGGCGGGGATGATGGGGCCCTCAAGA
TCTGGGACCTTCGGCAGTTCAAGTCTGGTTCCCCAGTGGCCACCTTCAAG
CAGCACGTGGCCCCCGTGACCTCCGTCGAGTGGCACCCCCAGGACAGCGG
GGTCTTTGCAGCCTCGGGTGCAGACCACCAGATCACACAGTGGGACCTGG
CAGTGGAGCGGGACCCTGAGGCGGGCGACGTGGAGGCCGACCCCGGACTG
GCCGACCTCCCGCAGCAGCTGCTGTTCGTGCACCAGGGCGAGACCGAGCT
GAAGGAGCTGCACTGGCACCCGCAGTGCCCAGGGCTCCTGGTCAGCACGG
CGCTGTCAGGCTTCACCATCTTCCGCACCATCAGCGTCTGAgcggccgc

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLASATMAEIGTGFPFDPHYVEV
LGERMHYVDVGPRDGTPVLFLHGNPTSSYVWRNIIPHVAPTHRCIAPDLI
GMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIHDWGSALGFHWAK
RNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQNV
FIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPAN
IVALVEEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDI
GPGLNLLQEDNPDLIGSEIARWLSTLEISGENLYFQGGSMAARKGRRRTC
ETGEPMEAESGDTSSEGPAQVYLPGRGPPLREGEELVMDEEAYVLYHRAQ
TGAPCLSFDIVRDHLGDNRTELPLTLYLCAGTQAESAQSNRLMMLRMHNL
HGTKPPPSEGSDEEEEEEDEEDEEERKPQLELAMVPHYGGINRVRVSWLG
EEPVAGVWSEKGQVEVFALRRLLQVVEEPQALAAFLRDEQAQMKPIFSFA
GHMGEGFALDWSPRVTGRLLTGDCQKNIHLWTPTDGGSWHVDQRPFVGHT
RSVEDLQWSPTENTVFASCSADASIRIWDIRAAPSKACMLTTATAHDGDV
NVISWSRREPFLLSGGDDGALKIWDLRQFKSGSPVATFKQHVAPVTSVEW
HPQDSGVFAASGADHQITQWDLAVERDPEAGDVEADPGLADLPQQLLFVH
QGETELKELHWHPQCPGLLVSTALSGFTIFRTISV*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU25033
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: silent mutation R8; Parental Plasmid DU24005