cDNA Clone RNF19ARBR

• Plasmid: pET15-6His-SUMO-RNF19A aa112-366
• Parent Plasmid: pET15b 6His SUMO1
• Expressed: 6His-SUMO-RNF19A aa112-366
• Genbank: NP_056250.3
• Tag: 6His
• Tag: SUMO
• Species: Human

Sequence of Insert

GGATCC
AATACCTCATCGGACAACGGCCTGACCTCCATCTCAAAACAAATCGGTGACTTCATCGAATGCCCGCTGTGCCTG
CTGCGCCACTCAAAAGATCGTTTTCCGGACATTATGACCTGCCATCACCGCAGCTGCGTCGATTGTCTGCGTCAG
TATCTGCGCATTGAAATCTCCGAATCACGTGTGAACATCTCGTGCCCGGAATGTACGGAACGTTTTAATCCGCAT
GACATTCGCCTGATCCTGAGCGATGACGTGCTGATGGAAAAATATGAAGAATTCATGCTGCGTCGCTGGCTGGTT
GCCGATCCGGACTGCCGTTGGTGTCCGGCACCGGATTGCGGCTACGCTGTTATTGCGTTTGGTTGCGCTAGCTGT
CCGAAACTGACCTGCGGCCGTGAAGGCTGTGGTACGGAATTCTGCTATCATTGTAAACAGATCTGGCACCCGAAC
CAAACCTGTGATGCAGCACGTCAGGAACGTGCACAATCCCTGCGTCTGCGCACGATTCGTAGCTCTAGTATCTCG
TATAGCCAGGAATCAGGTGCAGCTGCGGATGACATTAAACCGTGCCCGCGCTGTGCCGCATACATTATCAAAATG
AACGATGGCTCTTGCAATCATATGACCTGTGCCGTCTGCGGTTGTGAATTTTGCTGGCTGTGTATGAAAGAAATT
TCTGACCTGCACTACCTGTCTCCGAGTGGCTGTACGTTCTGGGGTAAAAAACCGTGGAGTCGTAAAAAGAAAATT
CTGTGGCAACTGGGC
TAAGCGGCCGC

Amino Acid Sequence

MGHHHHHHSDQEAKPSTEDLGDKKEGEYIKLKVIGQDSSEIHFKVKMTTH
LKKLKESYCQRQGVPMNSLRFLFEGQRIADNHTPKELGMEEEDVIEVYQE
QTGGSNTSSDNGLTSISKQIGDFIECPLCLLRHSKDRFPDIMTCHHRSCV
DCLRQYLRIEISESRVNISCPECTERFNPHDIRLILSDDVLMEKYEEFML
RRWLVADPDCRWCPAPDCGYAVIAFGCASCPKLTCGREGCGTEFCYHCKQ
IWHPNQTCDAARQERAQSLRLRTIRSSSISYSQESGAAADDIKPCPRCAA
YIIKMNDGSCNHMTCAVCGCEFCWLCMKEISDLHYLSPSGCTFWGKKPWS
RKKKILWQLG*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU24246
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: codon optimised for bacterial expression. linker to be deleted