cDNA Clone RNF19BRBR

• Plasmid: pET15-6His-SUMO-RNF19B aa100-352
• Parent Plasmid: pET15b 6His SUMO1
• Expressed: 6His-SUMO-RNF19B aa100-352
• Genbank: Uniprot Q6ZMZ0
• Tag: 6His
• Tag: SUMO
• Species: Human

Sequence of Insert

GGATCC
GGTTTTGACGACGAAGAAGCTGCCGAAGGTGGTGGTCCGGGTGCCGAAGAAGTGGAATGTCCGCTGTGCCTGGTC
CGCCTGCCGCCGGAACGTGCACCGCGTCTGCTGAGCTGCCCGCATCGTTCTTGCCGCGATTGTCTGCGTCACTAT
CTGCGCCTGGAAATTAGCGAATCTCGTGTCCCGATCAGTTGCCCGGAATGTTCCGAACGTCTGAACCCGCATGAT
ATTCGTCTGCTGCTGGCGGACCCGCCGCTGATGCACAAATATGAAGAATTTATGCTGCGTCGCTACCTGGCGTCA
GATCCGGACTGCCGTTGGTGTCCGGCACCGGATTGCGGTTATGCGGTTATTGCCTACGGCTGCGCCTCCTGTCCG
AAACTGACCTGCGAACGCGAAGGTTGTCAGACGGAATTCTGCTATCATTGTAAACAGATCTGGCACCCGAATCAA
ACCTGTGACATGGCACGTCAGCAACGCGCTCAGACGCTGCGTGTGCGCACCAAACATACGTCAGGCCTGTCGTAT
GGTCAAGAAAGCGGCCCGGCAGATGACATCAAACCGTGCCCGCGTTGTTCAGCTTACATTATCAAAATGAACGAT
GGTTCGTGCAATCATATGACCTGTGCGGTTTGCGGCTGTGAATTTTGCTGGCTGTGTATGAAAGAAATTAGCGAC
CTGCACTACCTGAGTCCGTCCGGTTGTACGTTCTGGGGCAAAAAACCGTGGTCTCGTAAAAAGAAAATTCTGTGG
CAGCTGGGC
TAAGCGGCCGC

Amino Acid Sequence

MGHHHHHHSDQEAKPSTEDLGDKKEGEYIKLKVIGQDSSEIHFKVKMTTH
LKKLKESYCQRQGVPMNSLRFLFEGQRIADNHTPKELGMEEEDVIEVYQE
QTGGSGFDDEEAAEGGGPGAEEVECPLCLVRLPPERAPRLLSCPHRSCRD
CLRHYLRLEISESRVPISCPECSERLNPHDIRLLLADPPLMHKYEEFMLR
RYLASDPDCRWCPAPDCGYAVIAYGCASCPKLTCEREGCQTEFCYHCKQI
WHPNQTCDMARQQRAQTLRVRTKHTSGLSYGQESGPADDIKPCPRCSAYI
IKMNDGSCNHMTCAVCGCEFCWLCMKEISDLHYLSPSGCTFWGKKPWSRK
KKILWQLG*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU24247
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: codon optimised for bacterial expression. linker to be deleted