cDNA Clone SGK1

• Synonyms: SGK
• Plasmid: pMK-RQ SGK1 Cter GFP donor
• Parent Plasmid: pMK-RQ
• Genbank: ENSG00000118515
• Tag: GFP
• Species: Human

Sequence of Insert

gcggccgcTGGTGAGTATCTGTCTCTCTTCTAAGTATAGAGAAGCCCAAAGGGCATTTATTTTAATTCAGAATTGTCTGGGGGAGGGTTGGAAGGAATACATTGGCAGATGTTTTCTCCATAAACCTGTTATTTTACCTACATAAAAAGCACATTTTTGTGTCCCAACAAGGCTCCCATAATTTTTAGACACATTTATCAATTCGAAGCACCAAAAGGCAACAAGTGAACATTATTCTTATGTTTAACTGTGTGTAGCCTTTTGAGATTTTGTGCTTGAAGTGGGTGATTATGGAAGTTGATATAAGACTTAAACTTGGTATTTAAAGCCTGGTCAAGATTTCCCTGTCCTGTGTCTAGTGTGAGTTCTTGACAAGAGTGTTTTTCCCTTCCCGTCACAGAGTGGGCCCAACGACCTACGGCACTTTGACCCCGAGTTTACCGAAGAGCCTGTCCCCAACTCCATTGGCAAGTCCCCTGACAGCGTCCTCGTCACAGCCAGCGTCAAGGAgGCaGCtGAaGCaTTCCTAGGCTTTTCCTATGCGCCTCCCACGGACTCTTTCCTCGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTGTCCGGCGAGGGCGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCATCTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCCTGACCTACGGCGTGCAGTGCTTCAGCCGCTACCCCGACCACATGAAGCAGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAGCGCACCATCTTCTTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGAAGTTCGAGGGCGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGACTTCAAGGAGGACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCAGAAGAACGGCATCAAGGTGAACTTCAAGATCCGCCACAACATCGAGGACGGCAGCGTGCAGCTCGCCGACCACTACCAGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGACAACCACTACCTGAGCACCCAGTCCGCCCTGAGCAAAGACCCCAACGAGAAGCGCGATCACATGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGATCACTCTCGGCATGGACGAGCTGTACAAGTGAACCCTGTTAGGGCTTGGTTTTAAAGGATTTTATGTGTGTTTCCGAATGTTTTAGTTAGCCTTTTGGTGGAGCCGCCAGCTGACAGGACATCTTACAAGAGAATTTGCACATCTCTGGAAGCTTAGCAATCTTATTGCACACTGTTCGCTGGAAGCTTTTTGAAGAGCACATTCTCCTCAGTGAGCTCATGAGGTTTTCATTTTTATTCTTCCTTCCAACGTGGTGCTATCTCTGAAACGAGCGTTAGAGTGCCGCCTTAGACGGAGGCAGGAGTTTCGTTAGAAAGCGGACGCTGTTCTAAAAAAGGTCTCCTGCAGATCTGTCTGGGCTGTGATGACGAATATTATGAAATGTGCCTTTTCTGAAGAGATTGTGTTAGCTCCAAAGCTTTTCCTATCGCAGTGTTTCAGTTCTTTATTTTCCCTTGTGGATATGCTGTGTGAACCGTCGTGTGAGTGTGGTATGCCTGATCACAGATGGATTTTGTTATAAGCATCgcggccgc

• Vector Type: Mammalian
• Antibiotic: Kan
• DU Number: DU48086
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: CRISPR donor designed to insert GFP to the c-terminal end of SGK1 via homologous recombination. Contains 500 bp flanking homology on either side of reporter.