cDNA Clone SGK1

• Synonyms: SGK
• Plasmid: pMS-RQ SGK1 Nter GFP donor
• Parent Plasmid: pMS-RQ
• Expressed: GFP (following recombination)
• Genbank: ENSG00000118515
• Tag: GFP
• Species: Human

Sequence of Insert

GCGCCAGGCCGTCCCCGGGCGGTCCTCGCGCCCCCGCCCGGGGCTCGCCCTCTCAATGGGGACAGAACCGCCCGCCGCAGGCAGCGTAGCCGCCAGCAAACCGCGAGGCGGCCGGGGCGGGGCGAGGGGCGAGGCGAAGGGCGGGGCCACTTCTCACTGTCGCGCAGGCCCCGCCCCCGCGGCGGTGCCTTTTTTATAAGGCCGAGCGCGCGGCCTGGCGCAGCATACGCCGAGCCGGTCTTTGAGCGCTAACGTCTTTCTGTCTCCCCGCGGTGGTGATGACGGTGAAAACTGAGGCTGCTAAGGGCACCCTCACTTACTCCAGGATGAGGGGCATGGTGGCAATTCTCATCGGTGAGTGCAGGAATCTTGCGGGACTTCTGCTCCAGGAGACGCAAAGTGGAAATTTTTTGAAAGTCCCGGATCAGATTAGTGTGTGTGGCGCCGGACGTTATGAAGCCGTCTAAACGTTTCTTTATTTCTCCTCCTTCATCCACAGCTTTtATGAAaCAaAGaAccGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTGTCCGGCGAGGGCGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCATCTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCCTGACCTACGGCGTGCAGTGCTTCAGCCGCTACCCCGACCACATGAAGCAGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAGCGCACCATCTTCTTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGAAGTTCGAGGGCGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGACTTCAAGGAGGACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCAGAAGAACGGCATCAAGGTGAACTTCAAGATCCGCCACAACATCGAGGACGGCAGCGTGCAGCTCGCCGACCACTACCAGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGACAACCACTACCTGAGCACCCAGTCCGCCCTGAGCAAAGACCCCAACGAGAAGCGCGATCACATGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGATCACTCTCGGCATGGACGAGCTGTACAAGtaaGGATGGGTCTGAACGACTTTATTCAGAAGATTGCCAATAACTCCTATGCATGCAAACAGTAAGTTTGACCGGATTTGAGGAAATAACTAGTATAGTTTGAATTTGCCAGCGGTAAACATTCTCATCACGGCGTTTATCGGGAAGGCGAAGACTTCTTCTGGGGTGGGGATCTCATTTCTCCTTAAATTCTAATATATTTGACACATTTTAAACATTAAAGTTAATTTGCTGATTTGGCTTGAACTGGAGATGTAAGATAAATGGTTCGTGTTGGCCGAATTCACGGCCTTTCTCCATGAGCAACAATCCTTATTTCTGTATTTAATGGGGTTTATTATTTTCTTTAACTGACTAATGTATTGGGGTATTTTCAGTTTAAACAGTGAATTATCCGGGTAGAAGTCGGTAGAGCCAGAAACTCACTTTTGATGTTGGTGTGCCCCCTAGTGGCGAGCTGGATTCTAAATCGTGCCCTTTATTCCCTGCAGCCCTGAAGTTCA

• Vector Type: Mammalian
• Antibiotic: Kan
• DU Number: DU48235
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Designed to insert an in-frame GFP into the first common coding exon (exon 2 isoform 1). Complete sequence of donor including homology arms shown as sequence of insert, contains several silent mutations to prevent Cas9 targeting the donor sequence. Successful recombination will knockout the SGK1 allele.