cDNA Clone SGK1

• Synonyms: SGK
• Plasmid: pMS-RQ SGK1 Nter GFP polyA donor
• Parent Plasmid: pMS-RQ
• Expressed: GFP (following recombination)
• Genbank: ENSG00000118515
• Tag: GFP
• Species: Human

Sequence of Insert

GCGGCCGCGCCAGGCCGTCCCCGGGCGGTCCTCGCGCCCCCGCCCGGGGCTCGCCCTCTCAATGGGGACAGAACCGCCCGCCGCAGGCAGCGTAGCCGCCAGCAAACCGCGAGGCGGCCGGGGCGGGGCGAGGGGCGAGGCGAAGGGCGGGGCCACTTCTCACTGTCGCGCAGGCCCCGCCCCCGCGGCGGTGCCTTTTTTATAAGGCCGAGCGCGCGGCCTGGCGCAGCATACGCCGAGCCGGTCTTTGAGCGCTAACGTCTTTCTGTCTCCCCGCGGTGGTGATGACGGTGAAAACTGAGGCTGCTAAGGGCACCCTCACTTACTCCAGGATGAGGGGCATGGTGGCAATTCTCATCGGTGAGTGCAGGAATCTTGCGGGACTTCTGCTCCAGGAGACGCAAAGTGGAAATTTTTTGAAAGTCCCGGATCAGATTAGTGTGTGTGGCGCCGGACGTTATGAAGCCGTCTAAACGTTTCTTTATTTCTCCTCCTTCATCCACAGCTTTTATGAAACAAAGAACCGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTGTCCGGCGAGGGCGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCATCTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCCTGACCTACGGCGTGCAGTGCTTCAGCCGCTACCCCGACCACATGAAGCAGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAGCGCACCATCTTCTTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGAAGTTCGAGGGCGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGACTTCAAGGAGGACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCAGAAGAACGGCATCAAGGTGAACTTCAAGATCCGCCACAACATCGAGGACGGCAGCGTGCAGCTCGCCGACCACTACCAGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGACAACCACTACCTGAGCACCCAGTCCGCCCTGAGCAAAGACCCCAACGAGAAGCGCGATCACATGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGATCACTCTCGGCATGGACGAGCTGTACAAGTGACTGTGCCTTCTAGTTGCCAGCCATCTGTTGTTTGCCCCTCCCCCGTGCCTTCCTTGACCCTGGAAGGTGCCACTCCCACTGTCCTTTCCTAATAAAATGAGGAAATTGCATCGCATTGTCTGAGTAGGTGTCATTCTATTCTGGGGGGTGGGGTGGGGCAGGACAGCAAGGGGGAGGATTGGGAAGACAATAGCAGGCATGCTGGGGATGCGGTGGGCTCTATGGGGATGGGTCTGAACGACTTTATTCAGAAGATTGCCAATAACTCCTATGCATGCAAACAGTAAGTTTGACCGGATTTGAGGAAATAACTAGTATAGTTTGAATTTGCCAGCGGTAAACATTCTCATCACGGCGTTTATCGGGAAGGCGAAGACTTCTTCTGGGGTGGGGATCTCATTTCTCCTTAAATTCTAATATATTTGACACATTTTAAACATTAAAGTTAATTTGCTGATTTGGCTTGAACTGGAGATGTAAGATAAATGGTTCGTGTTGGCCGAATTCACGGCCTTTCTCCATGAGCAACAATCCTTATTTCTGTATTTAATGGGGTTTATTATTTTCTTTAACTGACTAATGTATTGGGGTATTTTCAGTTTAAACAGTGAATTATCCGGGTAGAAGTCGGTAGAGCCAGAAACTCACTTTTGATGTTGGTGTGCCCCCTAGTGGCGAGCTGGATTCTAAATCGTGCCCTTTATTCCCTGCAGCCCTGAAGTTCAGCGGCCGC

• Vector Type: Mammalian
• Antibiotic: Kan
• DU Number: DU48298
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Designed to insert an in-frame GFP into the first common coding exon (exon 2 isoform 1). Complete sequence of donor including homology arms shown as sequence of insert, contains several silent mutations to prevent Cas9 targeting the donor sequence. Successful recombination will knockout the SGK1 allele. Added a polyA signal following the GFP insert to prevent degradation of the transcript via NMD.