cDNA Clone ubiquitin

• Plasmid: pSc-B-ubiquitin KallR
• Parent Plasmid: pSC-B
• Genbank: NM_020640
• Species: Human

Sequence of Insert

gcggccgctggcggctccggcggaggttccggaATGCAGATATTCGTGAG
GACCCTGACTGGTAGGACCATCACTCTCGAAGTGGAGCCGAGTGACACCA
TTGAGAATGTCAGGGCAAGGATCCAAGACAGGGAAGGCATCCCTCCTGAC
CAGCAGAGGTTGATCTTTGCTGGGAGACAGCTGGAAGATGGACGCACCCT
GTCTGACTACAACATCCAGAGAGAGTCCACCCTGCACCTGGTCCTCCGTC
TCAGAgctgcgTGAgcggccgc

Amino Acid Sequence

AAAGGSGGGSGMQIFVRTLTGRTITLEVEPSDTIENVRARIQDREGIPPD
QQRLIFAGRQLEDGRTLSDYNIQRESTLHLVLRLRAA*

• Vector Type: Other
• Antibiotic: Amp; Kan
• DU Number: DU24507
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Not1 ends with N-ter GGSGGGSG linker, plus stop. contains G75/76A substitutions at the C-teminus and a silent change @ nt9 (Ile3) to KO BglII site.