cDNA Clone SAE2

• Plasmid: pETDuet-1 GST Thrombin SAE1, 6-His Precission SAE 2
• Parent Plasmid: pETDuet1
• Expressed: 6His-SAE2
• Genbank: NM_005499.2
• Tag: 6His
• Species: Human

Sequence of Insert

CCATGGGACACCATCACCATCACCATCTGGAAGTTCTGTTCCAGGGGCCA
ATGGCACTGTCGCGGGGGCTGCCCCGGGAGCTGGCTGAGGCGGTGGCCGG
GGGCCGGGTGCTGGTGGTGGGGGCGGGCGGCATCGGCTGCGAGCTCCTCA
AGAATCTCGTGCTCACCGGTTTCTCCCACATCGACCTGATTGATCTGGAT
ACTATTGATGTAAGCAACCTCAACAGACAGTTTTTGTTTCAAAAGAAACA
TGTTGGAAGATCAAAGGCACAGGTTGCCAAGGAAAGTGTACTGCAGTTTT
ACCCGAAAGCTAATATCGTTGCCTACCATGACAGCATCATGAACCCTGAC
TATAATGTGGAATTTTTCCGACAGTTTATACTGGTTATGAATGCTTTAGA
TAACAGAGCTGCCCGAAACCATGTTAATAGAATGTGCCTGGCAGCTGATG
TTCCTCTTATTGAAAGTGGAACAGCTGGGTATCTTGGACAAGTAACTACT
ATCAAAAAGGGTGTGACCGAGTGTTATGAGTGTCATCCTAAGCCGACCCA
GAGAACCTTTCCTGGCTGTACAATTCGTAACACACCTTCAGAACCTATAC
ATTGCATCGTTTGGGCAAAGTACTTGTTCAACCAGTTGTTTGGGGAAGAA
GATGCTGATCAAGAAGTATCTCCTGACAGAGCTGACCCTGAAGCTGCCTG
GGAACCAACGGAAGCCGAAGCCAGAGCTAGAGCATCTAATGAAGATGGTG
ACATTAAACGTATTTCTACTAAGGAATGGGCTAAATCAACTGGATATGAT
CCAGTTAAACTTTTTACCAAGCTTTTTAAAGATGACATCAGGTATCTGTT
GACAATGGACAAACTATGGCGGAAAAGGAAACCTCCAGTTCCGTTGGACT
GGGCTGAAGTACAAAGTCAAGGAGAAGAAACGAATGCATCAGATCAACAG
AATGAACCCCAGTTAGGCCTGAAAGACCAGCAGGTTCTAGATGTAAAGAG
CTATGCACGTCTTTTTTCAAAGAGCATCGAGACTTTGAGAGTTCATTTAG
CAGAAAAGGGGGATGGAGCTGAGCTCATATGGGATAAGGATGACCCATCT
GCAATGGATTTTGTCACCTCTGCTGCAAACCTCAGGATGCATATTTTCAG
TATGAATATGAAGAGTAGATTTGATATCAAATCAATGGCAGGGAACATTA
TTCCTGCTATTGCTACTACTAATGCAGTAATTGCTGGGTTGATAGTATTG
GAAGGATTGAAGATTTTATCAGGAAAAATAGACCAGTGCAGAACAATTTT
TTTGAATAAACAACCAAACCCAAGAAAGAAGCTTCTTGTGCCTTGTGCAC
TGGATCCTCCCAACCCCAATTGTTATGTATGTGCCAGCAAGCCAGAGGTG
ACTGTGCGGCTGAATGTCCATAAAGTGACTGTTCTCACCTTACAAGACAA
GATAGTGAAAGAAAAATTTGCTATGGTAGCACCAGATGTCCAAATTGAAG
ATGGGAAAGGAACAATCCTAATATCTTCCGAAGAGGGAGAGACGGAAGCT
AATAATCACAAGAAGTTGTCAGAATTTGGAATTAGAAATGGCAGCCGGCT
TCAAGCAGATGACTTCCTCCAGGACTATACTTTATTGATCAACATCCTTC
ATAGTGAAGACCTAGGAAAGGACGTTGAATTTGAAGTTGTTGGTGATGCC
CCGGAAAAAGTGGGGCCCAAACAAGCTGAAGATGCTGCCAAAAGCATAAC
CAATGGCAGTGATGATGGAGCTCAGCCCTCCACCTCCACAGCTCAAGAGC
AAGATGACGTTCTCATAGTTGATTCAGATGAAGAAGATTCTTCAAATAAT
GCCGACGTCAGTGAAGAAGAGAGAAGCCGCAAGAGGAAATTAGATGAGAA
AGAGAATCTCAGTGCAAAGAGGTCACGTATAGAACAGAAGGAAGAGCTTG
ATGATGTCATAGCATTAGATTGAGTCGAC

Amino Acid Sequence

MGHHHHHHLEVLFQGPMALSRGLPRELAEAVAGGRVLVVGAGGIGCELLK
NLVLTGFSHIDLIDLDTIDVSNLNRQFLFQKKHVGRSKAQVAKESVLQFY
PKANIVAYHDSIMNPDYNVEFFRQFILVMNALDNRAARNHVNRMCLAADV
PLIESGTAGYLGQVTTIKKGVTECYECHPKPTQRTFPGCTIRNTPSEPIH
CIVWAKYLFNQLFGEEDADQEVSPDRADPEAAWEPTEAEARARASNEDGD
IKRISTKEWAKSTGYDPVKLFTKLFKDDIRYLLTMDKLWRKRKPPVPLDW
AEVQSQGEETNASDQQNEPQLGLKDQQVLDVKSYARLFSKSIETLRVHLA
EKGDGAELIWDKDDPSAMDFVTSAANLRMHIFSMNMKSRFDIKSMAGNII
PAIATTNAVIAGLIVLEGLKILSGKIDQCRTIFLNKQPNPRKKLLVPCAL
DPPNPNCYVCASKPEVTVRLNVHKVTVLTLQDKIVKEKFAMVAPDVQIED
GKGTILISSEEGETEANNHKKLSEFGIRNGSRLQADDFLQDYTLLINILH
SEDLGKDVEFEVVGDAPEKVGPKQAEDAAKSITNGSDDGAQPSTSTAQEQ
DDVLIVDSDEEDSSNNADVSEEERSRKRKLDEKENLSAKRSRIEQKEELD
DVIALD*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU20972
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial dual expression for Sae1 and 2. Sae2 was subcloned first into pET-Duet6P-1 MCS1 to give SC20936, and then Sae1 was subcloned into MCS2. Estimated protein MW of protein Sae2 : 73147 Daltons