cDNA Clone Exo5

• Synonyms: C1orf176; DEM1; Exo V; hExo5
• Plasmid: pGEX C3 HALO thr Exo5
• Parent Plasmid: pGEX C3 HALO thr
• Expressed: GST-HALO-exo5
• Genbank: Q9H790 (uniprot), NM_022774.1 GI:12232456 with coding change G172V: EST-derived and common variant
• Tag: GST
• Tag: HALO
• Species: Human

Sequence of Insert

GGATCCATGGCAGAGACAAGAGAAGAGGAGACAGTGTCAGCAGAAGCCTCAGGGTTCTCAGACTTGAGTGACTCAGAGTTCCTGGAGTTTCTGGACCTAGAAGATGCCCAAGAGTCAAAGGCTTTAGTTAACATGCCTGGCCCATCTTCTGAATCCCTTGGGAAGGATGACAAACCCATAAGCTTACAAAACTGGAAAAGAGGATTGGATATATTATCACCCATGGAGAGATTCCACCTTAAATATTTATATGTCACTGACCTGGCTACTCAGAACTGGTGTGAACTGCAAACAGCATATGGGAAGGAGCTTCCTGGTTTCTTGGCACCTGAGAAGGCAGCTGTGTTGGACACTGGTGCCAGCATACACCTAGCTAGAGAACTAGAACTTCATGATCTTGTGACTGTCCCAGTCACCACTAAAGAAGATGCTTGGGCAATTAAGTTTCTGAACATACTTTTGCTGATTCCTACCCTGCAGTCAGAAGGGCACATCAGAGAGTTTCCAGTGTTTGGGGAAGTGGAGGGTGTACTTCTTGTTGGAGTGATTGATGAGCTGCACTATACAGCCAAGGGGGAACTGGAGCTGGCGGAACTCAAGACACGCAGGCGCCCTATGCTCCCTCTGGAAGCTCAGAAGAAGAAAGACTGTTTTCAAGTCAGCCTATACAAATATATCTTTGATGCCATGGTACAAGGAAAAGTGACCCCTGCTAGCCTAATCCACCACACAAAGTTGTGTCTAGAAAAGCCACTGGGGCCATCAGTGCTGAGGCATGCCCAGCAGGGAGGCTTCTCTGTGAAGTCTTTGGGTGACCTCATGGAACTTGTCTTCTTGTCTCTAACACTGTCAGACCTCCCAGTTATTGATATCTTGAAGATTGAGTATATCCACCAAGAGACTGCCACTGTGCTGGGTACTGAGATTGTAGCCTTCAAAGAGAAGGAGGTGAGAGCCAAGGTGCAGCATTATATGGCCTACTGGATGGGCCACCGAGAGCCCCAGGGAGTTGACGTGGAGGAGGCTTGGAAGTGCCGGACGTGTACCTATGCAGACATTTGTGAGTGGAGAAAGGGCAGTGGAGTGCTCAGCTCTACACTGGCGCCCCAAGTCAAAAAAGCCAAATGAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMAEIGTGFPFDPHYVEVLG
ERMHYVDVGPRDGTPVLFLHGNPTSSYVWRNIIPHVAPTHRCIAPDLIGM
GKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIHDWGSALGFHWAKRN
PERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQNVFI
EGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIV
ALVEEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGP
GLNLLQEDNPDLIGSEIARWLSTLEISGLVPRGSGSMAETREEETVSAEA
SGFSDLSDSEFLEFLDLEDAQESKALVNMPGPSSESLGKDDKPISLQNWK
RGLDILSPMERFHLKYLYVTDLATQNWCELQTAYGKELPGFLAPEKAAVL
DTGASIHLARELELHDLVTVPVTTKEDAWAIKFLNILLLIPTLQSEGHIR
EFPVFGEVEGVLLVGVIDELHYTAKGELELAELKTRRRPMLPLEAQKKKD
CFQVSLYKYIFDAMVQGKVTPASLIHHTKLCLEKPLGPSVLRHAQQGGFS
VKSLGDLMELVFLSLTLSDLPVIDILKIEYIHQETATVLGTEIVAFKEKE
VRAKVQHYMAYWMGHREPQGVDVEEAWKCRTCTYADICEWRKGSGVLSST
LAPQVKKAK*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU24790
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee