cDNA Clone NIK

• Synonyms: Mitogen-activated protein kinase kinase kinase 14, MAP3K14
• Plasmid: pFBDual6P-1 NIK delta 78-84 D534A S814A S817A S819A
• Parent Plasmid: pFastbac dual 6P1
• Expressed: GST NIK delta 78-84 D534A S814A S817A S819A
• Genbank: Q99558
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCGCAGTGATGGAAATGGCCTGCCCAGGTGCCCCTGGCTCAGCAGT
GGGGCAGCAGAAGGAACTCCCCAAGCCAAAGGAGAAGACGCCGCCACTGG
GGAAGAAACAGAGCTCCGTCTACAAGCTTGAGGCCGTGGAGAAGAGCCCT
GTGTTCTGCGGAAAGTGGGAGATCCTGAATGACGTGATTACCAAGGGCAC
AGCCAAGGAAGGCTCCGAGGCAGGGCCAGCTGCCGAGTGTGAGAATAGCC
AAGAGTTCAGCCCCACCTTTTCAGAACGCATTTTCATCGCTGGGTCCAAA
CAGTACAGCCAGTCCGAGAGTCTTGATCAGATCCCCAACAATGTGGCCCA
TGCTACAGAGGGCAAAATGGCCCGTGTGTGTTGGAAGGGAAAGCGTCGCA
GCAAAGCCCGGAAGAAACGGAAGAAGAAGAGCTCAAAGTCCCTGGCTCAT
GCAGGAGTGGCCTTGGCCAAACCCCTCCCCAGGACCCCTGAGCAGGAGAG
CTGCACCATCCCAGTGCAGGAGGATGAGTCTCCACTCGGCGCCCCATATG
TTAGAAACACCCCGCAGTTCACCAAGCCTCTGAAGGAACCAGGCCTTGGG
CAACTCTGTTTTAAGCAGCTTGGCGAGGGCCTACGGCCGGCTCTGCCTCG
ATCAGAACTCCACAAACTGATCAGCCCCTTGCAATGTCTGAACCACGTGT
GGAAACTGCACCACCCCCAGGACGGAGGCCCCCTGCCCCTGCCCACGCAC
CCCTTCCCCTATAGCAGACTGCCTCATCCCTTCCCATTCCACCCTCTCCA
GCCCTGGAAACCTCACCCTCTGGAGTCCTTCCTGGGCAAACTGGCCTGTG
TAGACAGCCAGAAACCCTTGCCTGACCCACACCTGAGCAAACTGGCCTGT
GTAGACAGTCCAAAGCCCCTGCCTGGCCCACACCTGGAGCCCAGCTGCCT
GTCTCGTGGTGCCCATGAGAAGTTTTCTGTGGAGGAATACCTAGTGCATG
CTCTGCAAGGCAGCGTGAGCTCAAGCCAGGCCCACAGCCTGACCAGCCTG
GCCAAGACCTGGGCAGCAAGGGGCTCCAGATCCCGGGAGCCCAGCCCCAA
AACTGAGGACAACGAGGGTGTCCTGCTCACTGAGAAACTCAAGCCAGTGG
ATTATGAGTACCGAGAAGAAGTCCACTGGGCCACGCACCAGCTCCGCCTG
GGCAGAGGCTCCTTCGGAGAGGTGCACAGGATGGAGGACAAGCAGACTGG
CTTCCAGTGCGCTGTCAAAAAGGTGCGGCTGGAAGTATTTCGGGCAGAGG
AGCTGATGGCATGTGCAGGATTGACCTCACCCAGAATTGTCCCTTTGTAT
GGAGCTGTGAGAGAAGGGCCTTGGGTCAACATCTTCATGGAGCTGCTGGA
AGGTGGCTCCCTGGGCCAGCTGGTCAAGGAGCAGGGCTGTCTCCCAGAGG
ACCGGGCCCTGTACTACCTGGGCCAGGCCCTGGAGGGTCTGGAATACCTC
CACTCACGAAGGATTCTGCATGGGGACGTCAAAGCTGACAACGTGCTCCT
GTCCAGCGATGGGAGCCACGCAGCCCTCTGTGCCTTTGGCCATGCTGTGT
GTCTTCAACCTGATGGCCTGGGAAAGTCCTTGCTCACAGGGGACTACATC
CCTGGCACAGAGACCCACATGGCTCCGGAGGTGGTGCTGGGCAGGAGCTG
CGACGCCAAGGTGGATGTCTGGAGCAGCTGCTGTATGATGCTGCACATGC
TCAACGGCTGCCACCCCTGGACTCAGTTCTTCCGAGGGCCGCTCTGCCTC
AAGATTGCCAGCGAGCCTCCGCCTGTGAGGGAGATCCCACCCTCCTGCGC
CCCTCTCACAGCCCAGGCCATCCAAGAGGGGCTGAGGAAAGAGCCCATCC
ACCGCGTGTCTGCAGCGGAGCTGGGAGGGAAGGTGAACCGGGCACTACAG
CAAGTGGGAGGTCTGAAGAGCCCTTGGAGGGGAGAATATAAAGAACCAAG
ACATCCACCGCCAAATCAAGCCAATTACCACCAGACCCTCCATGCCCAGC
CGAGAGAGCTTTCGCCAAGGGCCCCAGGGCCCCGGCCAGCTGAGGAGACA
ACAGGCAGAGCCCCTAAGCTCCAGCCTCCTCTCCCACCAGAGCCCCCAGA
GCCAAACAAGTCTCCTCCCTTGACTTTGAGCAAGGAGGAGTCTGGGATGT
GGGAACCCTTACCTCTGTCCTCCCTGGAGCCAGCCCCTGCCAGAAACCCC
AGCTCACCAGAGCGGAAAGCAACCGTCCCGGAGCAGGAACTGCAGCAGCT
GGAAATAGAATTATTCCTCAACAGCCTGTCCCAGCCATTTTCTCTGGAGG
AGCAGGAGCAAATTCTCTCGTGCCTCAGCATCGACAGCCTCTCCCTGTCG
GATGACAGTGAGAAGAACCCAGCAAAGGCCGCTCAAGCCTCGCGGGACAC
CCTGAGCTCAGGCGTACACTCCTGGAGCAGCCAGGCCGAGGCTCGAAGCT
CCAGCTGGAACATGGTGCTGGCCCGGGGGCGGCCCACCGACACCCCAAGC
TATTTCAATGGTGTGAAAGTCCAAATACAGTCTCTTAATGGTGAACACCT
GCACATCCGGGAGTTCCACCGGGTCAAAGTGGGAGACATCGCCACTGGCA
TCAGCAGCCAGATCCCAGCTGCAGCCTTCAGCTTGGTGACCAAAGACGGG
CAGCCTGTTCGCTACGACATGGAGGTGCCAGACTCGGGCATCGACCTGCA
GTGCACACTGGCCCCTGATGGCAGCTTCGCCTGGAGCTGGAGGGTCAAGC
ATGGCCAGCTGGAGAACAGGCCCTAAGAATTC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSAVMEMACPGAPGSAVGQQK
ELPKPKEKTPPLGKKQSSVYKLEAVEKSPVFCGKWEILNDVITKGTAKEG
SEAGPAAECENSQEFSPTFSERIFIAGSKQYSQSESLDQIPNNVAHATEG
KMARVCWKGKRRSKARKKRKKKSSKSLAHAGVALAKPLPRTPEQESCTIP
VQEDESPLGAPYVRNTPQFTKPLKEPGLGQLCFKQLGEGLRPALPRSELH
KLISPLQCLNHVWKLHHPQDGGPLPLPTHPFPYSRLPHPFPFHPLQPWKP
HPLESFLGKLACVDSQKPLPDPHLSKLACVDSPKPLPGPHLEPSCLSRGA
HEKFSVEEYLVHALQGSVSSSQAHSLTSLAKTWAARGSRSREPSPKTEDN
EGVLLTEKLKPVDYEYREEVHWATHQLRLGRGSFGEVHRMEDKQTGFQCA
VKKVRLEVFRAEELMACAGLTSPRIVPLYGAVREGPWVNIFMELLEGGSL
GQLVKEQGCLPEDRALYYLGQALEGLEYLHSRRILHGDVKADNVLLSSDG
SHAALCAFGHAVCLQPDGLGKSLLTGDYIPGTETHMAPEVVLGRSCDAKV
DVWSSCCMMLHMLNGCHPWTQFFRGPLCLKIASEPPPVREIPPSCAPLTA
QAIQEGLRKEPIHRVSAAELGGKVNRALQQVGGLKSPWRGEYKEPRHPPP
NQANYHQTLHAQPRELSPRAPGPRPAEETTGRAPKLQPPLPPEPPEPNKS
PPLTLSKEESGMWEPLPLSSLEPAPARNPSSPERKATVPEQELQQLEIEL
FLNSLSQPFSLEEQEQILSCLSIDSLSLSDDSEKNPAKAAQASRDTLSSG
VHSWSSQAEARSSSWNMVLARGRPTDTPSYFNGVKVQIQSLNGEHLHIRE
FHRVKVGDIATGISSQIPAAAFSLVTKDGQPVRYDMEVPDSGIDLQCTLA
PDGSFAWSWRVKHGQLENRP*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU51078
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee