cDNA Clone UNC93B1

• Synonyms: IIAE1; UNC93; UNC93B; Unc-93B1
• Plasmid: pSC-B IRES2 UNC93B1
• Parent Plasmid: pSC-B
• Genbank: NM_030930.3
• Species: Human

Sequence of Insert

CCATGGAGGCGGAGCCGCCGCTCTACCCGATGGCGGGGGCTGCGGGGCCGCAGGGCGACGAGGACCTGCTCGGGGTCCCGGACGGGCCCGAGGCCCCGCTGGACGAGCTGGTGGGCGCGTACCCCAACTACAACGAGGAGGAGGAGGAGCGCCGCTACTACCGCCGCAAGCGCCTGGGCGTGCTCAAGAACGTGCTGGCTGCCAGCGCCGGGGGCATGCTCACCTACGGCGTCTACCTGGGCCTCCTGCAGATGCAGCTGATCCTGCACTACGACGAGACCTACCGCGAGGTGAAGTATGGCAACATGGGGCTGCCCGACATCGACAGCAAAATGCTGATGGGCATCAACGTGACTCCCATCGCCGCCCTGCTCTACACACCTGTGCTCATCAGGTTTTTTGGAACGAAGTGGATGATGTTCCTCGCTGTGGGCATCTACGCCCTCTTTGTCTCCACCAACTACTGGGAGCGCTACTACACGCTTGTGCCCTCGGCTGTGGCCCTGGGCATGGCCATCGTGCCTCTTTGGGCTTCAATGGGCAACTACATCACCAGGATGGCGCAGAAGTACCATGAGTACTCCCACTACAAGGAGCAGGATGGGCAGGGGATGAAGCAGCGGCCTCCGCGGGGCTCCCACGCGCCCTATCTCCTGGTCTTCCAAGCCATCTTCTACAGCTTCTTCCATCTGAGCTTCGCCTGCGCCCAGCTGCCCATGATTTATTTCCTGAACCACTACCTGTATGACCTGAACCACACGCTGTACAATGTGCAGAGCTGCGGCACCAACAGCCACGGGATCCTCAGCGGCTTCAACAAGACGGTTCTGCGGACGCTCCCGCGGAGCGGAAACCTCATTGTGGTGGAGAGCGTGCTCATGGCAGTGGCCTTCCTGGCCATGCTGCTGGTGCTGGGTTTGTGCGGAGCCGCTTACCGGCCCACGGAGGAGATCGATCTGCGCAGCGTGGGCTGGGGCAACATCTTCCAGCTGCCCTTCAAGCACGTGCGTGACTACCGCCTGCGCCACCTCGTGCCTTTCTTTATCTACAGCGGCTTCGAGGTGCTCTTTGCCTGCACTGGTATCGCCTTGGGCTATGGCGTGTGCTCGGTGGGGCTGGAGCGGCTGGCTTACCTCCTCGTGGCTTACAGCCTGGGCGCCTCAGCCGCCTCACTCCTGGGCCTGCTGGGCCTGTGGCTGCCACGCCCGGTGCCCCTGGTGGCCGGAGCAGGGGTGCACCTGCTGCTCACCTTCATCCTCTTTTTCTGGGCCCCTGTGCCTCGGGTCCTGCAACACAGCTGGATCCTCTATGTGGCAGCTGCCCTTTGGGGTGTGGGCAGTGCCCTGAACAAGACTGGACTCAGCACACTCCTGGGAATCTTGTACGAAGACAAGGAGAGACAGGACTTCATCTTCACCATCTACCACTGGTGGCAGGCTGTGGCCATCTTCACCGTGTACCTGGGCTCGAGCCTGCACATGAAGGCTAAGCTGGCGGTGCTGCTGGTGACGCTGGTGGCGGCCGCGGTCTCCTACCTGCGGATGGAGCAGAAGCTGCGGCGGGGCGTGGCCCCGCGCCAGCCCCGCATCCCGCGGCCCCAGCACAAGGTGCGCGGTTACCGCTACTTGGAGGAGGACAACTCGGACGAGAGCGACGCGGAGGGCGAGCATGGGGACGGCGCGGAGGAGGAGGCGCCGCCCGCAGGGCCCAGGCCTGGCCCCGAGCCCGCTGGACTCGGCCGCCGGCCCTGCCCGTACGAACAGGCGCAGGGGGGAGACGGGCCGGAGGAGCAGTGACCATGG

Amino Acid Sequence

MEAEPPLYPMAGAAGPQGDEDLLGVPDGPEAPLDELVGAYPNYNEEEEERRYYRRKRLGVLKNVLAASAGGMLTYGVYLGLLQMQLILHYDETYREVKYGNMGLPDIDSKMLMGINVTPIAALLYTPVLIRFFGTKWMMFLAVGIYALFVSTNYWERYYTLVPSAVALGMAIVPLWASMGNYITRMAQKYHEYSHYKEQDGQGMKQRPPRGSHAPYLLVFQAIFYSFFHLSFACAQLPMIYFLNHYLYDLNHTLYNVQSCGTNSHGILSGFNKTVLRTLPRSGNLIVVESVLMAVAFLAMLLVLGLCGAAYRPTEEIDLRSVGWGNIFQLPFKHVRDYRLRHLVPFFIYSGFEVLFACTGIALGYGVCSVGLERLAYLLVAYSLGASAASLLGLLGLWLPRPVPLVAGAGVHLLLTFILFFWAPVPRVLQHSWILYVAAALWGVGSALNKTGLSTLLGILYEDKERQDFIFTIYHWWQAVAIFTVYLGSSLHMKAKLAVLLVTLVAAAVSYLRMEQKLRRGVAPRQPRIPRPQHKVRGYRYLEEDNSDESDAEGEHGDGAEEEAPPAGPRPGPEPAGLGRRPCPYEQAQGGDGPEEQ*

• Vector Type: Other
• Antibiotic: Amp; Kan
• DU Number: DU26136
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Silent mutation at R519 and S178 (to KO internal NCO1 site); Non silent mutation to correct