cDNA Clone KLH28

• Synonyms: BTBD5, KLH28
• Plasmid: pGEX C3 HALO thr-KLHL28
• Parent Plasmid: pGEX C3 HALO thr
• Expressed: GST-HALO-KLHL28
• Genbank: Q9NXS3 (uniprot) Gene ID 54813 NM_017658.3 NP_060128.2
• Tag: GST
• Tag: HALO
• Species: Human

Sequence of Insert

ggatccATGGACCACACATCCCCGACCTACATGCTTGCTAACTTAACCCA
CTTGCATTCTGAACAACTTCTGCAGGGCTTGAATCTTCTTCGCCAACATC
ACGAACTCTGTGACATCATTCTTCGAGTAGGTGATGTTAAAATTCATGCT
CACAAAGTGGTACTTGCCAGCGTCAGCCCGTATTTCAAAGCTATGTTCAC
TGGAAACCTTTCTGAAAAAGAGAACAGTGAGGTTGAGTTTCAATGCATTG
ATGAAACTGCTCTCCAGGCCATTGTGGAGTATGCCTATACAGGGACTGTT
TTTATTTCTCAGGACACAGTTGAATCTCTCCTGCCAGCAGCAAACCTACT
CCAGATAAAACTTGTCCTGAAAGAATGTTGTGCATTTCTTGAAAGCCAAC
TTGATCCTGGTAATTGTATTGGAATTTCTCGTTTTGCAGAAACATATGGT
TGCCGTGACCTTTATTTGGCAGCCACTAAATACATATGCCAGAATTTTGA
AGCTGTTTGCCAGACTGAAGAGTTTTTTGAGCTTACACATGCTGACTTGG
ATGAAATTGTTTCCAATGACTGTTTGAATGTAGCTACCGAAGAGACTGTT
TTTTATGCATTAGAGTCTTGGATCAAGTATGATGTACAAGAACGCCAGAA
ATACTTAGCACAGTTACTAAACAGTGTACGATTACCATTGTTGAGTGTTA
AGTTTCTCACTAGACTATATGAAGCAAATCATCTTATTCGTGATGATCGC
ACTTGTAAACATCTTTTGAATGAAGCCCTAAAGTACCACTTTATGCCTGA
ACATAGACTCTCTCATCAGACAGTCTTGATGACACGACCTCGCTGTGCTC
CCAAAGTACTTTGTGCAGTAGGAGGGAAATCTGGACTCTTTGCCTGTTTG
GATAGTGTGGAGATGTACTTTCCTCAGAATGACTCTTGGATTGGTTTGGC
ACCCCTAAACATTCCTCGCTATGAATTTGGAATATGCGTTTTAGACCAAA
AAGTATATGTTATAGGTGGTATTGCAACTAATGTGCGTCCTGGCGTCACT
ATCAGAAAACATGAAAATTCAGTGGAATGCTGGAATCCTGATACAAATAC
TTGGACTTCTCTAGAGAGAATGAATGAAAGCCGAAGTACTCTTGGAGTAG
TAGTACTTGCAGGAGAACTTTATGCCTTAGGTGGTTATGATGGACAATCT
TATTTACAATCTGTAGAGAAGTACATTCCCAAAATAAGAAAATGGCAACC
TGTGGCACCAATGACGACAACAAGAAGTTGTTTTGCTGCAGCGGTATTGG
ATGGAATGATATATGCCATTGGTGGGTATGGTCCTGCCCACATGAACAGT
GTGGAGCGTTATGATCCAAGTAAGGACTCCTGGGAGATGGTTGCATCCAT
GGCAGATAAAAGGATTCACTTTGGCGTGGGTGTCATGCTAGGCTTTATTT
TTGTGGTGGGTGGACATAATGGAGTCTCACATTTGTCCAGCATTGAAAGA
TACGATCCTCATCAAAATCAGTGGACTGTGTGTAGACCAATGAAAGAACC
TAGAACAGGAGTTGGTGCTGCTGTAATCGATAACTACCTTTATGTCGTCG
GTGGTCACTCAGGGTCTTCCTATCTGAATACAGTGCAGAAATATGATCCT
ATCTCAGATACGTGGCTGGATTCAGCTGGCATGATATACTGTCGCTGCAA
CTTTGGGTTAACTGCACTTTGAgcggccgc

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMAEIGTGFPFDPHYVEVLG
ERMHYVDVGPRDGTPVLFLHGNPTSSYVWRNIIPHVAPTHRCIAPDLIGM
GKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIHDWGSALGFHWAKRN
PERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQNVFI
EGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIV
ALVEEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGP
GLNLLQEDNPDLIGSEIARWLSTLEISGLVPRGSGSMDHTSPTYMLANLT
HLHSEQLLQGLNLLRQHHELCDIILRVGDVKIHAHKVVLASVSPYFKAMF
TGNLSEKENSEVEFQCIDETALQAIVEYAYTGTVFISQDTVESLLPAANL
LQIKLVLKECCAFLESQLDPGNCIGISRFAETYGCRDLYLAATKYICQNF
EAVCQTEEFFELTHADLDEIVSNDCLNVATEETVFYALESWIKYDVQERQ
KYLAQLLNSVRLPLLSVKFLTRLYEANHLIRDDRTCKHLLNEALKYHFMP
EHRLSHQTVLMTRPRCAPKVLCAVGGKSGLFACLDSVEMYFPQNDSWIGL
APLNIPRYEFGICVLDQKVYVIGGIATNVRPGVTIRKHENSVECWNPDTN
TWTSLERMNESRSTLGVVVLAGELYALGGYDGQSYLQSVEKYIPKIRKWQ
PVAPMTTTRSCFAAAVLDGMIYAIGGYGPAHMNSVERYDPSKDSWEMVAS
MADKRIHFGVGVMLGFIFVVGGHNGVSHLSSIERYDPHQNQWTVCRPMKE
PRTGVGAAVIDNYLYVVGGHSGSSYLNTVQKYDPISDTWLDSAGMIYCRC
NFGLTAL*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU24822
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: parental 24192