cDNA Clone FancI(Drosophila)

• Synonyms: CG13745; Dmel\CG13745; fanci
• Plasmid: pBacPAK? 6His-GST 3C Drosophila FancI M1-K1411
• Expressed: 6His-GST 3C Drosophila FancI M1-K1411
• Genbank: NM_136585.3 GI:665399661 with changes - see notes
• Tag: GST
• Tag: 6His
• Species: Drosophila Melanogaster

Amino Acid Sequence

MGHHHHHHGSTMSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKER
AEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQI
DKYLKSSKYIAWPLQGWQATFGGGDHPPKSDLEVLFQGPLSLDPCRRPMRSLGEKGRFDELELLIQNTPIEKLKETLKSTMTRSEGITYWNYLLRGFNPE
SKDALAKRFECVRSFMDGLSSTELSYKQTFDLITRLSQDLATFPSEQLAWIVEHCMDGIRQGDAKCVGWKDLLPDALSLLLARPRFLLNGISIDGMEFRD
TTVRSIATMQWPASILTPIADMFKTMNLSSGEIVTVLNKFSGALQELSPMELPALCFQLFSMCSTASQLIITLLALEKYFHRNYYKRLFSDMCSNSTNLD
SIDLFSDKELREAEETILHHLNYCTMYKLTEKHLAIMLRNFLHMPDVILTPFMLSAIISMTSINRDPESARISHSILLPFLRNVIKNNEEERTLADYSVW
YRDTLQRKQVDLQQVLTVLIDQNKDGKDVITPGLVHLVFYLLKSQSPKMHTLAITFLTKFIRKRFIFGQGIIKLISEWMIVYQEQNQFSECLTLLSVADT
YTVSECMKPIQSVLEHMQWLSGEQSMRMMNFILPLLKISNRVRDALIDVLCKAMSSSGIQKRRMAIYGFCMILKQLNNSNAVRQTSSATSFCTQHSISGY
SIMTQNTLGSRSNPQRNFDMLTLEIIGMLRNCLQQQFDIRCSLYENLQRAVELNAKLVPHVLQVIDWHFRSFFDTPSAEDAGDDLDTVFRIRYDQLVSSS
DDQHMEIQLKDNLGKLIQFVANCLAVFDRAPSGYDTREMNRLMSFCVDRMVANRLPLEEITPPATHLKCALVLQQLNIIEGIICHLLLKSKPQNNAVCQI
LPLFNQHCKLLESLKALSDASKKAHKQLKQTAKNNSTSTCNLTLNGVPVKTMCTQPDNIWDLAILDKLLHLLLDDVVAFAAPEKTVLLRSNEPLVRYVLS
VTASRVESIRLEPDYKQLAYSKRTFKQLTDITKVIYERCIQRLPELWKNFDMQSAALATQCFSECLKTAHETYSKKFEDFVKSFDLASIKGSRNMEVVYT
IQDVLDDFMTEYGTDDSICKNEFVSKLPVYLLESLEILLDHIDYQDRAATESYTWLLNFCSKNEILNSEMGLVHRMLFVQRQKTHLGPFFDSVARQLGHV
LGVQNEDAPSESVELTLKSISTVTIESCLQHLYAAIQKQLNDVDYFVTKANNLNYKCQVVPEIDQIYWRGNLDSMDRSICTQIIHISRTLLVLTNVCIPL
GSLMDGLMKLLIQHYTCLKSLTKHYISSYTSDTSIENIKSTKFELLLRAVGKQLPANIYELITYIEANTLDEEAQQHTKKRKVQAERAKVLRETRLIPKV
IMVIEDFNKHIILLSKKTKSKNRLTDYLHLGTMRDFDIKSTDLRAAIERSISDGRNISTEDSNAEDRDEDEDENENVEEDDDAQSTTYPEDLEEKEDECL
VIEKKEERRRRRAPSSEAEDPQPKRKRGRKAAEQLEAEDEEQVEEEQPETTLKLRRNKKSGAKNQKECGH*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU50185
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Provided by Mark Frost (Helen Walden group); ; Various silent changes, plus residue differences:; A8G; P224T; K947M.; Extra residues at C-terminus: SGAKNQKECGH*; ; Recommend growth at 30 degrees.