cDNA Clone HOIP

• Synonyms: ring finger protein 31 (RNF31) (HOIP)
• Plasmid: pCDFDuet-1-6His-HOIP UBA-RBR domain/ HOIL-1L aa M1-E191
• Parent Plasmid: pCDFDUET-1
• Expressed: 6His-HOIP UBA-RBR domain (aa474-1072)
• Genbank: NM_017999.4
• Tag: 6His
• Species: Human

Sequence of Insert

CCATGGGCAGCAGCCATCATCATCATCATCACAGCAGCGGCCTGGAAGTT
CTGTTCCAGGGGGGAGATCCTGAGAAGCAGCGCCAAGACAAGATGCGGGA
AGAAGGCCTCCAGCTAGTGAGCATGATCCGGGAAGGGGAAGCCGCAGGTG
CCTGTCCAGAGGAGATCTTCTCGGCTCTGCAGTACTCGGGCACTGAGGTG
CCTCTGCAGTGGTTGCGCTCAGAACTGCCCTACGTCCTGGAGATGGTGGC
TGAGCTGGCTGGACAGCAGGACCCTGGGCTGGGTGCCTTTTCCTGTCAGG
AGGCCCGGAGAGCCTGGCTGGATCGTCATGGCAACCTTGATGAAGCTGTG
GAGGAGTGTGTGAGGACCAGGCGAAGGAAGGTGCAGGAGCTCCAGTCTCT
AGGCTTTGGGCCTGAGGAGGGGTCTCTCCAGGCATTGTTCCAGCACGGAG
GTGATGTGTCACGGGCCCTGACTGAGCTACAGCGCCAACGCCTAGAGCCC
TTCCGCCAGCGCCTCTGGGACAGTGGCCCTGAGCCCACCCCTTCCTGGGA
TGGGCCAGACAAGCAGAGCCTGGTCAGGCGGCTTTTGGCAGTCTACGCAC
TCCCCAGCTGGGGCCGGGCAGAGCTGGCACTGTCACTGCTGCAGGAGACA
CCCAGGAACTATGAGTTGGGGGATGTGGTAGAAGCTGTGAGGCACAGCCA
GGACCGGGCCTTCCTGCGCCGCTTGCTTGCCCAGGAGTGTGCCGTGTGTG
GCTGGGCCCTGCCCCACAACCGGATGCAGGCCCTGACTTCCTGTGAGTGC
ACCATCTGTCCTGACTGCTTCCGCCAGCACTTCACCATCGCCTTGAAGGA
GAAGCACATCACAGACATGGTGTGCCCTGCCTGTGGCCGCCCCGACCTCA
CCGATGACACACAGTTGCTCAGCTACTTCTCTACCCTTGACATCCAGCTT
CGCGAGAGCCTAGAGCCAGATGCCTATGCGTTGTTCCATAAGAAGCTGAC
CGAGGGTGTGCTGATGCGGGACCCCAAGTTCTTGTGGTGTGCCCAGTGCT
CCTTTGGCTTCATATATGAGCGTGAGCAGCTGGAGGCAACTTGTCCCCAG
TGTCACCAGACCTTCTGTGTGCGCTGCAAGCGCCAGTGGGAGGAGCAGCA
CCGAGGTCGGAGCTGTGAGGACTTCCAGAACTGGAAACGCATGAACGACC
CAGAATACCAGGCCCAGGGCCTAGCAATGTATCTTCAGGAAAACGGCATT
GACTGCCCCAAATGCAAGTTCTCGTACGCCCTGGCCCGAGGAGGCTGCAT
GCACTTTCACTGTACCCAGTGCCGCCACCAGTTCTGCAGCGGCTGCTACA
ATGCCTTTTACGCCAAGAATAAATGTCCAGAGCCTAACTGCAGGGTGAAA
AAGTCCCTGCACGGCCACCACCCTCGAaACTGCCTCTTCTACCTGCGGGA
CTGGACTGCTCTCCGGCTTCAGAAGCTGCTACAGGACAATAACGTCATGT
TTAATACAGAGCCTCCAGCTGGGGCCCGGGCAGTCCCTGGAGGCGGCTGC
CGAGTGATAGAGCAGAAGGAGGTTCCCAATGGGCTCAGGGACGAAGCTTG
TGGCAAGGAAACTCCAGCTGGCTATGCCGGCCTGTGCCAGGCACACTACA
AAGAGTATCTTGTGAGCCTCATCAATGCCCACTCGCTGGACCCAGCCACC
TTGTATGAGGTGGAAGAGCTGGAGACGGCCACTGAGCGCTACCTGCACGT
ACGCCCCCAGCCTTTGGCTGGAGAGGATCCCCCTGCTTACCAGGCCCGCT
TGTTACAGAAGCTGACAGAAGAGGTACCCTTGGGACAGAGTATCCCCCGC
AGGCGGAAGTAGGCGGCCGC

Amino Acid Sequence

MGSSHHHHHHSSGLEVLFQGGDPEKQRQDKMREEGLQLVSMIREGEAAGA
CPEEIFSALQYSGTEVPLQWLRSELPYVLEMVAELAGQQDPGLGAFSCQE
ARRAWLDRHGNLDEAVEECVRTRRRKVQELQSLGFGPEEGSLQALFQHGG
DVSRALTELQRQRLEPFRQRLWDSGPEPTPSWDGPDKQSLVRRLLAVYAL
PSWGRAELALSLLQETPRNYELGDVVEAVRHSQDRAFLRRLLAQECAVCG
WALPHNRMQALTSCECTICPDCFRQHFTIALKEKHITDMVCPACGRPDLT
DDTQLLSYFSTLDIQLRESLEPDAYALFHKKLTEGVLMRDPKFLWCAQCS
FGFIYEREQLEATCPQCHQTFCVRCKRQWEEQHRGRSCEDFQNWKRMNDP
EYQAQGLAMYLQENGIDCPKCKFSYALARGGCMHFHCTQCRHQFCSGCYN
AFYAKNKCPEPNCRVKKSLHGHHPRNCLFYLRDWTALRLQKLLQDNNVMF
NTEPPAGARAVPGGGCRVIEQKEVPNGLRDEACGKETPAGYAGLCQAHYK
EYLVSLINAHSLDPATLYEVEELETATERYLHVRPQPLAGEDPPAYQARL
LQKLTEEVPLGQSIPRRRK*

• Vector Type: Bacterial
• Antibiotic: Spectinomycin
• DU Number: DU24935
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: KO'd internal Xho1 site at nt2790 (>CTCGAA) relative to full-length sequence