cDNA Clone USP25

• Plasmid: pGEX C3 HALO thr-USP25 C178A
• Parent Plasmid: pGEX C3 HALO thr
• Expressed: GST-HALO-USP25 C178A
• Genbank: NP_037528.3 [Q9UHP3 (uniprot)]
• Tag: HALO
• Tag: GST
• Species: Human

Sequence of Insert

ggatccATGACCGTGGAGCAGAACGTGCTGCAGCAGAGCGCGGCGCAGAA
GCACCAGCAGACGTTTTTGAATCAACTGAGAGAAATTACGGGGATTAATG
ACACCCAGATACTACAGCAAGCCTTGAAGGATAGTAATGGAAACTTGGAA
TTAGCAGTGGCTTTCCTTACTGCGAAGAATGCTAAGACCCCTCAGCAGGA
GGAGACAACTTACTACCAAACAGCACTTCCTGGCAATGATAGATACATCA
GTGTGGGAAGCCAAGCAGATACAAATGTGATTGATCTCACTGGAGATGAT
AAAGATGATCTTCAGAGAGCAATTGCCTTGAGTTTGGCCGAATCAAACAG
GGCATTCAGGGAGACTGGAATAACTGATGAGGAACAAGCCATTAGCAGAG
TTCTTGAAGCCAGCATAGCAGAGAATAAAGCATGTTTGAAGAGGACACCT
ACAGAAGTTTGGAGGGATTCTCGAAACCCTTATGATAGAAAAAGACAGGA
CAAAGCTCCCGTTGGGCTAAAGAATGTTGGCAATACTgctTGGTTTAGTG
CTGTTATTCAGTCATTATTTAATCTTTTGGAATTTAGAAGATTAGTTCTG
AATTACAAGCCTCCATCAAATGCTCAAGATTTACCCCGAAACCAAAAGGA
ACATCGGAATTTGCCTTTTATGCGTGAGCTGAGGTATCTATTTGCACTTC
TTGTTGGTACCAAAAGGAAGTATGTTGATCCATCAAGAGCAGTTGAAATT
CTTAAGGATGCTTTCAAATCAAATGACTCACAGCAGCAAGATGTGAGTGA
GTTTACACACAAATTATTAGATTGGTTAGAAGATGCCTTCCAAATGAAAG
CTGAAGAGGAGACGGATGAAGAGAAGCCAAAGAACCCCATGGTAGAGTTG
TTCTATGGCAGATTCCTGGCTGTGGGAGTACTTGAAGGTAAAAAATTTGA
AAACACTGAAATGTTTGGTCAGTACCCACTTCAGGTCAATGGGTTCAAAG
ATCTGCATGAGTGCCTAGAAGCTGCAATGATTGAAGGAGAAATTGAGTCT
TTACATTCAGAGAATTCAGGAAAATCAGGCCAAGAGCATTGGTTTACTGA
ATTACCACCTGTGTTAACATTTGAATTGTCAAGATTTGAATTTAATCAGG
CATTGGGAAGACCAGAAAAAATTCACAACAAATTAGAATTTCCCCAAGTT
TTATATTTGGACAGATACATGCACAGAAACAGAGAAATAACAAGAATTAA
GAGGGAAGAGATCAAGAGACTGAAAGATTACCTCACGGTATTACAACAAA
GGCTAGAAAGATATTTAAGCTATGGTTCCGGTCCCAAACGATTCCCCTTG
GTAGATGTTCTTCAGTATGCATTGGAATTTGCCTCAAGTAAACCTGTTTG
CACTTCTCCTGTTGACGATATTGACGCTAGTTCCCCACCTAGTGGTTCCA
TACCATCACAGACATTACCAAGCACAACAGAACAACAGGGAGCCCTATCT
TCAGAACTGCCAAGCACATCACCTTCATCAGTTGCTGCCATTTCATCGAG
ATCAGTAATACACAAACCATTTACTCAGTCCCGGATACCTCCAGATTTGC
CCATGCATCCGGCACCGAGGCACATAACGGAGGAAGAACTTTCTGTGCTG
GAAAGTTGTTTACATCGCTGGAGGACAGAAATAGAAAATGACACCAGAGA
TTTGCAGGAAAGCATATCCAGAATCCATCGAACAATTGAATTAATGTACT
CTGACAAATCTATGATACAAGTTCCTTATCGATTACATGCCGTTTTAGTT
CACGAAGGCCAAGCTAATGCTGGGCACTACTGGGCATATATTTTTGATCA
TCGTGAAAGCAGATGGATGAAGTACAATGATATTGCTGTGACAAAATCAT
CATGGGAAGAGCTAGTGAGGGACTCTTTTGGTGGTTATAGAAATGCCAGT
GCATACTGTTTAATGTACATAAATGATAAGGCACAGTTCCTAATACAAGA
GGAGTTTAATAAAGAAACTGGGCAGCCCCTTGTTGGTATAGAAACATTAC
CACCGGATTTGAGAGATTTTGTTGAGGAAGACAACCAACGATTTGAAAAA
GAACTAGAAGAATGGGATGCACAACTTGCCCAGAAAGCTTTGCAGGAAAA
GCTTTTAGCGTCTCAGAAATTGAGAGAGTCAGAGACTTCTGTGACAACAG
CACAAGCAGCAGGAGACCCAGAATATCTAGAGCAGCCATCAAGAAGTGAT
TTCTCAAAGCACTTGAAAGAAGAAACTATTCAAATAATTACCAAGGCATC
ACATGAGCATGAAGATAAAAGTCCTGAAACAGTTTTGCAGTCGGCAATTA
AGTTGGAATATGCAAGGTTGGTTAAGTTGGCCCAAGAAGACACCCCACCA
GAAACCGATTATCGTTTACATCATGTAGTGGTCTACTTTATCCAGAACCA
GGCACCAAAGAAAATTATTGAGAAAACATTACTAGAACAATTTGGAGATA
GAAATTTGAGTTTTGATGAAAGGTGTCACAACATAATGAAAGTTGCTCAA
GCCAAACTGGAAATGATAAAACCTGAAGAAGTAAACTTGGAGGAATATGA
GGAGTGGCATCAGGATTATAGGAAATTCAGGGAAACAACTATGTATCTCA
TAATTGGGCTAGAAAATTTTCAAAGAGAAAGTTATATAGATTCCTTGCTG
TTCCTCATCTGTGCTTATCAGAATAACAAAGAACTCTTGTCTAAAGGCTT
ATACAGAGGACATGATGAAGAATTGATATCACATTATAGAAGAGAATGTT
TGCTAAAATTAAATGAGCAAGCCGCAGAACTCTTCGAATCTGGAGAGGAT
CGAGAAGTAAACAATGGTTTGATTATCATGAATGAGTTTATTGTCCCATT
TTTGCCATTATTACTGGTGGATGAAATGGAAGAAAAGGATATACTAGCTG
TAGAAGATATGAGAAATCGATGGTGTTCCTACCTTGGTCAAGAAATGGAA
CCACACCTCCAAGAAAAGCTGACAGATTTTTTGCCAAAACTGCTTGATTG
TTCTATGGAGATTAAAAGTTTCCATGAGCCACCGAAGTTACCTTCATATT
CCACGCATGAACTCTGTGAGCGATTTGCCCGAATCATGTTGTCCCTCAGT
CGAACTCCTGCTGATGGAAGATAAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMAEIGTGFPFDPHYVEVLG
ERMHYVDVGPRDGTPVLFLHGNPTSSYVWRNIIPHVAPTHRCIAPDLIGM
GKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIHDWGSALGFHWAKRN
PERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQNVFI
EGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIV
ALVEEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGP
GLNLLQEDNPDLIGSEIARWLSTLEISGLVPRGSGSMTVEQNVLQQSAAQ
KHQQTFLNQLREITGINDTQILQQALKDSNGNLELAVAFLTAKNAKTPQQ
EETTYYQTALPGNDRYISVGSQADTNVIDLTGDDKDDLQRAIALSLAESN
RAFRETGITDEEQAISRVLEASIAENKACLKRTPTEVWRDSRNPYDRKRQ
DKAPVGLKNVGNTAWFSAVIQSLFNLLEFRRLVLNYKPPSNAQDLPRNQK
EHRNLPFMRELRYLFALLVGTKRKYVDPSRAVEILKDAFKSNDSQQQDVS
EFTHKLLDWLEDAFQMKAEEETDEEKPKNPMVELFYGRFLAVGVLEGKKF
ENTEMFGQYPLQVNGFKDLHECLEAAMIEGEIESLHSENSGKSGQEHWFT
ELPPVLTFELSRFEFNQALGRPEKIHNKLEFPQVLYLDRYMHRNREITRI
KREEIKRLKDYLTVLQQRLERYLSYGSGPKRFPLVDVLQYALEFASSKPV
CTSPVDDIDASSPPSGSIPSQTLPSTTEQQGALSSELPSTSPSSVAAISS
RSVIHKPFTQSRIPPDLPMHPAPRHITEEELSVLESCLHRWRTEIENDTR
DLQESISRIHRTIELMYSDKSMIQVPYRLHAVLVHEGQANAGHYWAYIFD
HRESRWMKYNDIAVTKSSWEELVRDSFGGYRNASAYCLMYINDKAQFLIQ
EEFNKETGQPLVGIETLPPDLRDFVEEDNQRFEKELEEWDAQLAQKALQE
KLLASQKLRESETSVTTAQAAGDPEYLEQPSRSDFSKHLKEETIQIITKA
SHEHEDKSPETVLQSAIKLEYARLVKLAQEDTPPETDYRLHHVVVYFIQN
QAPKKIIEKTLLEQFGDRNLSFDERCHNIMKVAQAKLEMIKPEEVNLEEY
EEWHQDYRKFRETTMYLIIGLENFQRESYIDSLLFLICAYQNNKELLSKG
LYRGHDEELISHYRRECLLKLNEQAAELFESGEDREVNNGLIIMNEFIVP
FLPLLLVDEMEEKDILAVEDMRNRWCSYLGQEMEPHLQEKLTDFLPKLLD
CSMEIKSFHEPPKLPSYSTHELCERFARIMLSLSRTPADGR*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU24978
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: 24192 parental