cDNA Clone DFCP1

• Plasmid: pGEX C3 HALO thrombin-DFCP1
• Parent Plasmid: pGEX C3 HALO thrombin
• Expressed: GST-HALO-DFCP1
• Genbank: NP_067083.1
• Tag: HALO
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGAGTGCCCAGACTTCCCCAGCAGAGAAGGGCCTGAATCCGGGGCTGATGTGCCAGGAAAGTTACGCTTGCAGCGGGACTGATGAAGCTATCTTTGAGTGTGATGAGTGCTGCAGTCTGCAGTGTCTCCGCTGCGAGGAGGAGCTCCATCGGCAGGAGCGCCTGAGAAACCATGAGCGGATAAGACTCAAACCTGGCCATGTCCCTTACTGTGACCTCTGCAAGGGTCTCAGTGGGCATTTACCAGGTGTTAGGCAGAGGGCAATAGTGAGGTGCCAGACCTGCAAAATTAACTTGTGCCTGGAGTGCCAGAAGAGGACTCATTCTGGGGGTAACAAAAGGAGACACCCTGTTACTGTGTACAATGTCAGTAATCTCCAGGAGTCACTGGAGGCAGAAGAGATGGATGAGGAGACCAAGAGGAAGAAGATGACTGAGAAGGTTGTGAGTTTCCTCCTAGTAGACGAAAATGAAGAAATTCAGGTAACAAATGAAGAAGACTTTATTAGAAAATTGGACTGCAAACCTGATCAGCATCTGAAAGTGGTTTCCATTTTTGGAAATACTGGTGATGGAAAGTCTCATACTCTCAACCACACTTTCTTTTATGGTCGTGAAGTCTTTAAAACCTCCCCGACCCAGGAGTCCTGCACTGTGGGAGTGTGGGCAGCCTATGACCCAGTTCACAAAGTAGCAGTGATCGATACGGAAGGGCTCCTGGGGGCCACCGTGAATCTAAGCCAGAGAACACGGCTGCTGCTTAAGGTCCTGGCCATCTCAGACCTCGTCATCTATCGAACTCATGCAGACCGGCTGCATAACGACCTCTTCAAATTCCTTGGGGATGCCTCAGAAGCTTATCTGAAGCACTTCACCAAGGAGCTCAAGGCCACCACTGCTCGCTGTGGCCTGGATGTCCCTTTATCCACACTGGGCCCTGCAGTTATCATCTTCCATGAGACCGTGCACACCCAGCTACTGGGCTCTGATCATCCCTCAGAGGTGCCAGAGAAGCTCATCCAGGACCGGTTCCGGAAGCTGGGCCGTTTCCCTGAAGCCTTTAGTTCCATTCACTACAAGGGAACGAGGACTTACAACCCTCCCACGGACTTTTCTGGGCTTCGGCGTGCTTTGGAGCAGCTACTAGAGAATAACACCACCCGTTCTCCCCGGCACCCGGGAGTCATCTTCAAAGCCCTGAAGGCACTAAGTGACCGCTTCAGCGGTGAGATCCCCGATGACCAGATGGCGCACAGCTCCTTTTTTCCAGATGAGTATTTCACCTGCTCCTCCTTGTGCCTCAGCTGTGGGGTTGGATGTAAGAAAAGCATGAATCATGGGAAGGAAGGAGTGCCTCATGAAGCCAAGAGCCGCTGCAGATACTCCCACCAGTATGACAACCGAGTGTATACCTGCAAGGCCTGCTATGAGAGAGGCGAGGAAGTCAGTGTAGTGCCCAAAACATCTGCTTCCACTGACTCCCCCTGGATGGGTCTCGCAAAATATGCCTGGTCTGGGTATGTGATCGAATGTCCTAACTGTGGCGTGGTCTATCGTAGTCGGCAGTACTGGTTTGGAAACCAAGATCCTGTGGATACGGTGGTGCGGACAGAGATTGTGCATGTGTGGCCTGGAACTGATGGGTTTCTGAAGGACAACAACAATGCTGCCCAGCGCCTGTTGGACGGGATGAACTTCATGGCTCAGTCGGTGTCCGAGCTTAGCCTTGGACCCACCAAGGCTGTGACTTCCTGGCTGACAGACCAGATCGCCCCTGCCTACTGGAGGCCCAACTCCCAGATTCTGAGCTGCAACAAGTGTGCGACGTCCTTTAAAGATAACGACACTAAGCATCACTGCCGAGCCTGTGGGGAGGGCTTCTGTGACAGCTGTTCATCAAAGACTCGGCCAGTGCCTGAGCGGGGCTGGGGCCCTGCGCCAGTGCGGGTCTGTGACAACTGCTACGAAGCCAGGAACGTCCAGTTAGCTGTTACCGAGGCACAAGTGGACGATGAAGGTGGAACGCTCATTGCTCGGAAGGTGGGCGAGGCCGTGCAGAACACTCTGGGAGCCGTGGTGACAGCCATTGACATACCACTAGGTCTGGTAAAGGACGCGGCCAGGCCTGCGTACTGGGTGCCTGACCACGAAATCCTCCACTGCCACAACTGCCGGAAGGAGTTCAGCATCAAGCTCTCCAAGCACCACTGCCGGGCCTGCGGACAGGGCTTCTGTGATGAGTGCTCCCATGACCGCCGGGCTGTTCCTTCTCGTGGCTGGGACCATCCCGTCCGAGTCTGCTTCAACTGCAATAAAAAGCCCGGTGACCTTTAAGAATTCGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMAEIGTGFPFDPHYVEVLG
ERMHYVDVGPRDGTPVLFLHGNPTSSYVWRNIIPHVAPTHRCIAPDLIGM
GKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIHDWGSALGFHWAKRN
PERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQNVFI
EGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIV
ALVEEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGP
GLNLLQEDNPDLIGSEIARWLSTLEISGLVPRGSGSMSAQTSPAEKGLNP
GLMCQESYACSGTDEAIFECDECCSLQCLRCEEELHRQERLRNHERIRLK
PGHVPYCDLCKGLSGHLPGVRQRAIVRCQTCKINLCLECQKRTHSGGNKR
RHPVTVYNVSNLQESLEAEEMDEETKRKKMTEKVVSFLLVDENEEIQVTN
EEDFIRKLDCKPDQHLKVVSIFGNTGDGKSHTLNHTFFYGREVFKTSPTQ
ESCTVGVWAAYDPVHKVAVIDTEGLLGATVNLSQRTRLLLKVLAISDLVI
YRTHADRLHNDLFKFLGDASEAYLKHFTKELKATTARCGLDVPLSTLGPA
VIIFHETVHTQLLGSDHPSEVPEKLIQDRFRKLGRFPEAFSSIHYKGTRT
YNPPTDFSGLRRALEQLLENNTTRSPRHPGVIFKALKALSDRFSGEIPDD
QMAHSSFFPDEYFTCSSLCLSCGVGCKKSMNHGKEGVPHEAKSRCRYSHQ
YDNRVYTCKACYERGEEVSVVPKTSASTDSPWMGLAKYAWSGYVIECPNC
GVVYRSRQYWFGNQDPVDTVVRTEIVHVWPGTDGFLKDNNNAAQRLLDGM
NFMAQSVSELSLGPTKAVTSWLTDQIAPAYWRPNSQILSCNKCATSFKDN
DTKHHCRACGEGFCDSCSSKTRPVPERGWGPAPVRVCDNCYEARNVQLAV
TEAQVDDEGGTLIARKVGEAVQNTLGAVVTAIDIPLGLVKDAARPAYWVP
DHEILHCHNCRKEFSIKLSKHHCRACGQGFCDECSHDRRAVPSRGWDHPV
RVCFNCNKKPGDL*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU28013
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Parental 24192