cDNA Clone HACE1

• Plasmid: pcDNA5-FRT/TO-HACE1
• Parent Plasmid: pcDNA5D FRT/TO C-HA
• Expressed: HACE1
• Genbank: Q8IYU2
• Species: Human

Sequence of Insert

ggatccATGGAGAGAGCGATGGAGCAACTCAACCGCCTGACGCGCTCGCTGCGCCGCGCGCGCACCGTGGAGTTGCCCGAGGATAATGAAACTGCTGTTTATACATTAATGCCAATGGTTATGGCTGATCAACACAGGTCTGTTTCTGAACTACTATCAAATTCAAAATTTGATGTCAATTATGCATTCGGACGTGTGAAAAGAAGCTTGCTTCACATTGCAGCAAATTGTGGATCGGTGGAATGCTTGGTTTTGCTGTTAAAGAAAGGAGCAAATCCTAACTATCAAGATATTTCAGGCTGTACACCCCTTCATTTGGCAGCAAGAAATGGGCAGAAGAAATGTATGAGTAAATTATTAGAATATAGCGCTGATGTCAACATTTGTAATAATGAAGGCCTTACAGCAATACATTGGCTGGCTGTGAATGGGCGGACAGAACTACTCCATGACCTTGTGCAGCATGTCAGTGATGTTGATGTTGAGGATGCCATGGGGCAGACAGCACTGCATGTTGCCTGCCAGAACGGTCACAAGACGACAGTGCAGTGCTTGCTAGACAGTGGTGCTGATATTAACAGGCCAAATGTATCAGGAGCAACTCCATTGTACTTTGCTTGCAGTCATGGTCAGAGAGATACAGCACAGATCCTACTATTACGAGGAGCCAAATATCTGCCAGATAAAAATGGAGTAACTCCTCTGGATTTATGTGTACAGGGTGGATATGGAGAGACTTGTGAAGTATTAATTCAATATCACCCGAGGCTTTTTCAGACTATTATTCAAATGACACAGAATGAAGACCTCCGAGAAAACATGTTACGGCAAGTTCTGGAGCATTTGTCTCAGCAAAGTGAAAGCCAGTACCTAAAGATTCTAACAAGCCTTGCTGAAGTTGCTACAACAAATGGTCATAAACTGCTTAGCCTCTCTAGCAATTATGATGCTCAAATGAAGAGCCTTTTAAGGATTGTGAGAATGTTTTGTCACGTCTTTCGAATTGGTCCATCCTCCCCCAGTAATGGAATTGATATGGGCTACAATGGGAATAAAACTCCAAGAAGCCAGGTGTTCAAGCCTCTGGAATTGCTTTGGCACTCGTTAGATGAATGGCTAGTTTTAATAGCCACAGAATTGATGAAAAACAAAAGAGACTCAACAGAGATCACTTCTATTTTACTGAAACAAAAAGGCCAAGATCAAGATGCTGCTTCCATTCCTCCATTTGAACCTCCAGGACCTGGGAGCTATGAAAATCTGTCCACTGGCACAAGGGAATCTAAACCAGATGCTCTTGCAGGGAGACAGGAAGCCAGTGCAGATTGTCAGGATGTTATTTCTATGACAGCTAACCGGCTAAGTGCTGTCATTCAAGCTTTTTACATGTGCTGTTCTTGTCAGATGCCTCCGGGAATGACTTCACCTCGTTTCATTGAATTTGTCTGCAAACATGATGAAGTTTTAAAATGCTTTGTTAATAGAAATCCCAAAATTATATTTGACCACTTTCACTTTCTCCTTGAATGTCCTGAGTTGATGTCAAGATTCATGCATATCATAAAAGCACAGCCTTTTAAAGATCGCTGTGAATGGTTCTATGAACATTTGCATTCAGGACAGCCAGATTCAGATATGGTGCACAGGCCAGTGAATGAAAATGATATCCTGCTGGTTCACAGAGATTCTATTTTTAGGAGTAGCTGTGAAGTTGTGTCAAAAGCAAATTGTGCAAAGCTAAAGCAAGGGATTGCTGTACGGTTCCATGGAGAAGAAGGCATGGGTCAAGGTGTTGTGCGTGAGTGGTTTGATATTCTGTCCAATGAGATAGTCAATCCTGATTATGCATTGTTTACCCAGTCAGCTGATGGAACAACTTTTCAGCCTAATAGCAACTCTTATGTAAATCCTGATCACTTGAACTATTTTCGGTTTGCTGGGCAGATCTTGGGATTAGCGTTGAACCACAGGCAGCTGGTCAATATTTACTTCACACGATCCTTCTACAAGCACATTCTTGGTATTCCTGTAAATTACCAAGATGTGGCATCCATTGATCCAGAATATGCGAAAAATTTGCAATGGATTTTAGATAATGATATAAGTGATCTGGGTCTAGAACTAACTTTTTCTGTTGAGACTGATGTGTTTGGAGCAATGGAAGAGGTGCCTTTGAAACCTGGGGGTGGGAGTATTCTTGTGACACAAAATAATAAAGCGGAGTACGTCCAGCTTGTTACTGAACTTCGAATGACAAGAGCCATTCAGCCTCAGATCAATGCTTTTTTACAGGGCTTTCATATGTTCATTCCACCCTCCCTCATACAGCTTTTTGATGAATATGAATTGGAGCTACTGCTTTCTGGCATGCCAGAAATTGATGTGAGTGATTGGATAAAAAATACAGAATACACAAGTGGCTATGAAAGAGAAGATCCAGTTATTCAGTGGTTCTGGGAAGTTGTAGAAGACATTACTCAAGAGGAGAGAGTTCTTCTCTTACAGTTTGTTACGGGCAGTTCCAGGGTCCCACATGGTGGGTTTGCTAATATCATGGGTGGAAGTGGATTGCAAAACTTTACAATCGCTGCTGTGCCATATACTCCAAATCTTTTACCAACTTCAAGCACATGCATCAACATGCTCAAGTTACCTGAATACCCAAGTAAAGAAATACTCAAGGACAGACTTCTTGTGGCACTACATTGTGGCAGCTATGGTTACACAATGGCATAAgcggccgc

Amino Acid Sequence

MERAMEQLNRLTRSLRRARTVELPEDNETAVYTLMPMVMADQHRSVSELLSNSKFDVNYAFGRVKRSLLHIAANCGSVECLVLLLKKGANPNYQDISGCTPLHLAARNGQKKCMSKLLEYSADVNICNNEGLTAIHWLAVNGRTELLHDLVQHVSDVDVEDAMGQTALHVACQNGHKTTVQCLLDSGADINRPNVSGATPLYFACSHGQRDTAQILLLRGAKYLPDKNGVTPLDLCVQGGYGETCEVLIQYHPRLFQTIIQMTQNEDLRENMLRQVLEHLSQQSESQYLKILTSLAEVATTNGHKLLSLSSNYDAQMKSLLRIVRMFCHVFRIGPSSPSNGIDMGYNGNKTPRSQVFKPLELLWHSLDEWLVLIATELMKNKRDSTEITSILLKQKGQDQDAASIPPFEPPGPGSYENLSTGTRESKPDALAGRQEASADCQDVISMTANRLSAVIQAFYMCCSCQMPPGMTSPRFIEFVCKHDEVLKCFVNRNPKIIFDHFHFLLECPELMSRFMHIIKAQPFKDRCEWFYEHLHSGQPDSDMVHRPVNENDILLVHRDSIFRSSCEVVSKANCAKLKQGIAVRFHGEEGMGQGVVREWFDILSNEIVNPDYALFTQSADGTTFQPNSNSYVNPDHLNYFRFAGQILGLALNHRQLVNIYFTRSFYKHILGIPVNYQDVASIDPEYAKNLQWILDNDISDLGLELTFSVETDVFGAMEEVPLKPGGGSILVTQNNKAEYVQLVTELRMTRAIQPQINAFLQGFHMFIPPSLIQLFDEYELELLLSGMPEIDVSDWIKNTEYTSGYEREDPVIQWFWEVVEDITQEERVLLLQFVTGSSRVPHGGFANIMGGSGLQNFTIAAVPYTPNLLPTSSTCINMLKLPEYPSKEILKDRLLVALHCGSYGYTMA*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU28089
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Parental 41459, but with stop codon at end of ORF to make untagged