cDNA Clone USP49

• Plasmid: pcDNA5D FRT/TO HA-USP49 C262A
• Parent Plasmid: pcDNA FRT TO HA-MCS-BirA
• Expressed: HA-USP49 C262A
• Tag: HA
• Species: Human

Sequence of Insert

ATGTACCCATACGATGTGCCAGATTACGCCGGATCTATGGATAGATGCAA
ACATGTAGGGCGGTTACGGCTCGCCCAGGACCACTCCATCCTGAACCCTC
AGAAGTGGTGCTGCTTAGAGTGTGCCACCACCGAGTCCGTGTGGGCCTGC
CTCAAGTGCTCCCACGTGGCCTGCGGCCGCTATATTGAGGACCACGCCCT
GAAACACTTTGAGGAGACGGGACACCCGCTAGCCATGGAAGTCCGGGATC
TCTACGTGTTCTGTTACCTGTGCAAGGACTACGTGCTCAATGATAACCCA
GAGGGGGACCTGAAGCTGCTAAGAAGCTCCCTCCTGGCGGTCCGGGGCCA
GAAACAGGACACGCCGGTGAGACGTGGGCGGACGCTGCGGTCCATGGCTT
CGGGTGAGGACGTGGTCCTGCCGCAGCGCGCTCCTCAGGGACAGCCGCAG
ATGCTCACGGCTCTGTGGTACCGGCGTCAGCGCCTGCTGGCCAGGACGCT
GCGGCTGTGGTTCGAGAAGAGCTCCCGGGGCCAGGCGAAGCTGGAGCAGC
GGCGGCAGGAGGAGGCCCTGGAGCGCAAGAAGGAGGAGGCGCGGAGGCGG
CGGCGCGAGGTGAAACGGCGGCTGCTGGAGGAGCTGGCCAGCACCCCTCC
GCGCAAGAGTGCACGGCTGCTCCTGCACACGCCCCGCGACGCGGGCCCGG
CTGCCTCGCGCCCCGCCGCCCTCCCTACCTCACGCAGAGTGCCCGCCGCC
ACACTCAAGCTGCGTCGCCAGCCGGCCATGGCCCCAGGCGTCACGGGCCT
GCGCAACCTGGGCAACACCGCCTACATGAACTCCATCCTCCAGGTGCTCA
GCCACCTCCAGAAGTTCCGAGAATGTTTCCTCAACCTTGACCCTTCCAAA
ACGGAACATCTGTTTCCCAAAGCCACCAACGGGAAGACTCAGCTTTCTGG
CAAGCCAACCAACAGCTCGGCCACGGAGCTGTCCTTGAGAAATGACAGGG
CCGAGGCATGCGAGCGGGAGGGCTTCTGCTGGAACGGCAGGGCCTCCATT
AGTCGGAGTCTGGAGCTCATCCAGAACAAGGAGCCGAGTTCAAAGCACAT
TTCCCTCTGCCGTGAACTGCACACCCTCTTCCGAGTCATGTGGTCCGGGA
AGTGGGCCCTAGTGTCGCCCTTCGCCATGCTGCACTCAGTGTGGAGCCTG
ATCCCTGCCTTCCGCGGCTACGACCAACAGGACGCGCAGGAATTTCTCTG
CGAGCTGCTGCACAAGGTGCAGCAGGAACTCGAGTCTGAGGGCACCACAC
GCCGGATCCTCATCCCCTTCTCCCAGAGGAAGCTCACCAAACAGGTCTTA
AAGGTGGTGAATACCATATTTCATGGGCAGCTGCTCAGTCAGGTCACATG
TATATCATGCAATTACAAATCCAATACCATTGAGCCCTTTTGGGACCTAT
CCCTGGAATTCCCTGAACGCTATCACTGCATAGAAAAGGGGTTTGTCCCT
TTGAATCAAACAGAGTGCTTGCTCACTGAGATGCTGGCCAAATTCACAGA
GACAGAGGCCCTGGAAGGGAGAATCTACGCTTGTGACCAGTGTAACAGCA
AACGACGAAAATCCAATCCCAAACCCCTTGTTCTGAGTGAAGCTAGAAAG
CAGTTAATGATCTACAGACTACCTCAGGTTCTCCGGCTGCACCTTAAAAG
ATTCAGGTGGTCTGGCCGTAATCATCGAGAGAAGATTGGGGTCCATGTCG
TCTTTGACCAGGTATTAACCATGGAACCTTACTGCTGCAGGGACATGCTC
TCCTCTCTTGACAAAGAGACCTTTGCCTATGATCTCTCCGCAGTGGTCAT
GCATCACGGGAAAGGGTTTGGCTCAGGACACTACACAGCCTATTGCTACA
ACACAGAGGGAGGTGCGTGCGCTTTACTCTGTGGGGTGGGGGACACGGAA
AGGGGTTGAGATCCCCCGGGGAATTCGCTAGCCTCGAGAAGCTTGCGGCC
GCTATGAAGGACAACACCGTGCCCCTGAAGCTGATCGCCCTGCTGGCCAA
CGGCGAGTTCCACTCTGGCGAGCAGCTGGGAGAGACCCTGGGAATGAGCA
GAGCCGCCATCAACAAGCACATCCAGACACTGAGAGACTGGGGAGTGGAC
GTGTTCACCGTGCCTGGCAAGGGCTACAGCCTGCCTGAGCCTATCCAGCT
GCTGAACGCCAAGCAGATCCTGGGACAGCTGGATGGCGGAAGCGTGGCCG
TGCTGCCTGTGATCGACTCCACCAATCAGTACCTGCTGGACAGAATCGGA
GAGCTGAAGTCCGGCGACGCCTGCATCGCCGAGTACCAGCAGGCTGGCAG
AGGAGGCAGAGGACGGAAGTGGTTCAGCCCATTCGGAGCCAACCTGTACC
TGTCCATGTTCTGGAGACTGGAGCAGGGACCTGCTGCTGCCATCGGACTG
AGTCTGGTGATCGGAATCGTGATGGCCGAGGTGCTGAGAAAGCTGGGAGC
CGACAAGGTGAGAGTGAAGTGGCCTAATGACCTGTACCTCCAGGACCGCA
AGCTGGCTGGCATCCTGGTGGAGCTGACAGGCAAGACAGGCGATGCCGCT
CAGATCGTGATCGGAGCCGGAATCAACATGGCCATGAGAAGAGTGGAGGA
GAGCGTGGTGAACCAGGGCTGGATCACCCTGCAGGAGGCTGGCATCAACC
TGGACCGGAACACCCTGGCCGCCATGCTGATCAGAGAGCTGAGAGCCGCT
CTGGAGCTGTTCGAGCAGGAGGGACTGGCTCCTTACCTGAGCAGATGGGA
GAAGCTGGACAACTTCATCAACAGACCTGTGAAGCTGATCATCGGCGACA
AGGAAATCTTCGGCATCTCCAGAGGAATCGACAAGCAGGGAGCTCTGCTG
CTGGAGCAGGACGGAATCATCAAGCCCTGGATGGGCGGAGAAATCTCCCT
GAGAAGCGCAGAGAAGTGA

Amino Acid Sequence

MYPYDVPDYAGSMDRCKHVGRLRLAQDHSILNPQKWCCLECATTESVWAC
LKCSHVACGRYIEDHALKHFEETGHPLAMEVRDLYVFCYLCKDYVLNDNP
EGDLKLLRSSLLAVRGQKQDTPVRRGRTLRSMASGEDVVLPQRAPQGQPQ
MLTALWYRRQRLLARTLRLWFEKSSRGQAKLEQRRQEEALERKKEEARRR
RREVKRRLLEELASTPPRKSARLLLHTPRDAGPAASRPAALPTSRRVPAA
TLKLRRQPAMAPGVTGLRNLGNTAYMNSILQVLSHLQKFRECFLNLDPSK
TEHLFPKATNGKTQLSGKPTNSSATELSLRNDRAEACEREGFCWNGRASI
SRSLELIQNKEPSSKHISLCRELHTLFRVMWSGKWALVSPFAMLHSVWSL
IPAFRGYDQQDAQEFLCELLHKVQQELESEGTTRRILIPFSQRKLTKQVL
KVVNTIFHGQLLSQVTCISCNYKSNTIEPFWDLSLEFPERYHCIEKGFVP
LNQTECLLTEMLAKFTETEALEGRIYACDQCNSKRRKSNPKPLVLSEARK
QLMIYRLPQVLRLHLKRFRWSGRNHREKIGVHVVFDQVLTMEPYCCRDML
SSLDKETFAYDLSAVVMHHGKGFGSGHYTAYCYNTEGGACALLCGVGDTE
RG*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU28133
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Parental 49114. Mutagenesis required to remove STOP at end of USP49 and restore frame with C-terminal BirA tag