cDNA Clone USP1

• Synonyms: UBP
• Plasmid: pFBDM6P1 USP1 S55-L785(end) G670A G671A
• Parent Plasmid: pFBDM6P1
• Expressed: GST-3C-USP1 S55-L785(end) G670A G671A
• Genbank: NM_001017415.1 GI:63053521
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCTCTGAAATTGATCAAGTTGTTCCTGCAGCACAGTCTTCACCTATAAACTGTGAGAAGAGAGAAAACTTGTTACCATTTGTGGGACTGAATAATCTCGGCAATACTTGCTATCTTAATAGTATACTTCAGGTATTATATTTTTGTCCCGGTTTTAAATCTGGAGTAAAGCACTTATTTAATATTATTTCAAGGAAGAAAGAAGCTCTAAAGGATGAAGCCAATCAAAAAGACAAGGGAAATTGCAAAGAAGATTCTTTGGCAAGTTATGAATTGATATGCAGTTTACAGTCCTTAATCATTTCGGTTGAACAGCTCCAGGCTAGTTTTCTCTTAAATCCAGAGAAATATACTGATGAACTTGCCACTCAGCCAAGGCGACTGCTTAACACACTGAGGGAACTCAACCCTATGTATGAAGGATATCTACAGCATGATGCACAGGAAGTATTACAATGTATTTTGGGAAACATTCAAGAAACATGCCAACTCCTAAAAAAAGAAGAAGTAAAAAATGTGGCAGAATTACCTACTAAGGTAGAAGAAATACCTCATCCGAAAGAGGAAATGAATGGTATTAACAGCATAGAGATGGACAGTATGAGGCATTCTGAAGACTTTAAAGAGAAACTCCCAAAAGGAAATGGGAAAAGAAAAAGTGACACTGAATTTGGTAACATGAAGAAAAAAGTTAAATTATCCAAGGAACACCAGTCATTGGAAGAGAACCAGAGACAAACTAGATCAAAAAGAAAAGCTACAAGTGATACATTAGAGAGTCCTCCTAAAATAATTCCCAAGTATATTTCTGAAAATGAGAGTCCAAGACCCTCACAAAAGAAATCAAGAGTTAAAATAAATTGGTTAAAGTCTGCAACTAAGCAACCCAGCATTCTTTCTAAATTTTGTAGTCTGGGAAAAATAACAACAAACCAAGGAGTCAAAGGACAATCTAAAGAAAATGAATGTGATCCTGAAGAGGACTTGGGGAAGTGTGAAAGTGATAACACAACTAATGGTTGTGGACTTGAATCTCCAGGAAATACTGTTACACCTGTAAATGTTAATGAAGTTAAACCCATAAACAAAGGTGAAGAACAAATTGGTTTTGAGCTAGTGGAGAAATTATTTCAAGGTCAGCTGGTATTAAGGACGCGTTGCTTGGAATGTGAAAGTTTAACAGAAAGAAGAGAAGATTTTCAAGACATCAGTGTGCCAGTACAAGAAGATGAGCTTTCCAAAGTAGAGGAGAGTTCTGAAATTTCTCCAGAGCCAAAAACAGAAATGAAGACCCTGAGATGGGCAATTTCACAATTTGCTTCAGTAGAAAGGATTGTAGGAGAAGATAAATATTTCTGTGAAAACTGCCATCATTATACTGAAGCTGAACGAAGTCTTTTGTTTGACAAAATGCCTGAAGTTATAACTATTCATTTGAAGTGCTTTGCTGCTAGTGGTTTGGAGTTTGATTGTTATGGTGGTGGACTTTCCAAGATCAACACTCCTTTATTGACACCTCTTAAATTGTCACTAGAAGAATGGAGCACAAAGCCAACTAACGACAGCTATGGATTATTTGCGGTTGTGATGCATAGTGGCATTACAATTAGTAGTGGGCATTACACTGCTTCTGTTAAAGTCACTGACCTTAACAGTTTAGAACTAGATAAAGGAAATTTTGTGGTTGACCAAATGTGTGAAATAGGTAAGCCAGAACCATTGAATGAGGAGGAAGCAAGGGGTGTGGTTGAGAATTATAATGATGAAGAAGTGTCAATTAGAGTTGGTGGAAATACACAGCCAAGTAAAGTTTTGAACAAAAAAAATGTAGAAGCTATTGGACTTCTTGCCGCTCAAAAGAGCAAAGCAGATTATGAGCTATACAACAAAGCCTCTAATCCTGATAAGGTTGCTAGTACAGCGTTTGCTGAAAATAGAAATTCTGAGACTAGTGATACTACTGGGACCCATGAATCTGATAGAAACAAGGAATCCAGTGACCAAACAGGCATTAATATTAGTGGATTTGAGAACAAAATTTCATACGTAGTGCAAAGCTTAAAGGAGTATGAGGGGAAGTGGTTGCTTTTTGATGATTCTGAAGTCAAAGTTACTGAAGAGAAGGACTTTCTGAATTCTCTTTCCCCTTCTACATCTCCTACTTCTACTCCTTACTTGCTATTTTATAAGAAATTATAGcggccgc

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSSEIDQVVPAAQSSPINCEKRENLLPFVGLNNLGNTCYLNSILQVLYFCPGFKSGVKHLFNIISRKKEAL
KDEANQKDKGNCKEDSLASYELICSLQSLIISVEQLQASFLLNPEKYTDELATQPRRLLNTLRELNPMYEGYLQHDAQEVLQCILGNIQETCQLLKKEEV
KNVAELPTKVEEIPHPKEEMNGINSIEMDSMRHSEDFKEKLPKGNGKRKSDTEFGNMKKKVKLSKEHQSLEENQRQTRSKRKATSDTLESPPKIIPKYIS
ENESPRPSQKKSRVKINWLKSATKQPSILSKFCSLGKITTNQGVKGQSKENECDPEEDLGKCESDNTTNGCGLESPGNTVTPVNVNEVKPINKGEEQIGF
ELVEKLFQGQLVLRTRCLECESLTERREDFQDISVPVQEDELSKVEESSEISPEPKTEMKTLRWAISQFASVERIVGEDKYFCENCHHYTEAERSLLFDK
MPEVITIHLKCFAASGLEFDCYGGGLSKINTPLLTPLKLSLEEWSTKPTNDSYGLFAVVMHSGITISSGHYTASVKVTDLNSLELDKGNFVVDQMCEIGK
PEPLNEEEARGVVENYNDEEVSIRVGGNTQPSKVLNKKNVEAIGLLAAQKSKADYELYNKASNPDKVASTAFAENRNSETSDTTGTHESDRNKESSDQTG
INISGFENKISYVVQSLKEYEGKWLLFDDSEVKVTEEKDFLNSLSPSTSPTSTPYLLFYKKL*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU53238
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Please add a GST-3C for N-terminal truncated USP1 (S55-L785) and insert His-TEV-UAF1 (DU53076) please, thank you.