cDNA Clone ARIH1

• Synonyms: ariadne-1 homolog, ARI, ARI-1, HARI, HHARI, MOP-6, UBCH7BP, ubcM4-interacting protein, ubcH7-binding protein
• Plasmid: pcDNA5-FRT/TO-GFP ARIH1 (siRNA resistant)
• Parent Plasmid: pcDNA5 FRT/TO
• Expressed: GFP ARIH1
• Genbank: NP_005735
• Tag: GFP
• Species: Human

Sequence of Insert

GGATCCATGGACTCGGACGAGGGCTACAACTACGAGTTCGACGAGGACGA
GGAGTGCAGTGAGGAGGACAGCGGCGCCGAGGAGGAGGAGGACGAAGACG
ACGACGAGCCGGACGATGATACCCTGGATCTGGGCGAGGTGGAGCTGGTG
GAGCCCGGGCTGGGCGTCGGCGGGGAGCGGGACGGACTGCTGTGCGGGGA
GACGGGCGGTGGCGGCGGCAGCGCTCTGGGGCCCGGCGGTGGCGGCGGCG
GCGGCGGCGGCGGTGGTGGTGGCGGGCCGGGGCATGAGCAGGAGGAGGAT
TACCGCTACGAGGTGCTCACGGCCGAGCAGATTCTACAACACATGGTGGA
ATGTATCCGGGAGGTCAACGAGGTCATCCAGAATCCAGCAACTATCACAA
GAATACTCCTTAGCCACTTCAATTGGGATAAAGAGAAGCTAATGGAAAGG
TACTTTGATGGAAACCTGGAGAAGCTCTTTGCTGAGTGTCATGTAATTAA
TCCAAGTAAAAAGTCTCGAACACGCCAGATGAATACAAGGTCATCAGCAC
AGGATATGCCTTGTCAGATCTGCTACTTGAACTACCCTAACTCGTATTTC
ACTGGCCTTGAATGTGGACATAAGTTTTGTATGCAGTGCTGGAGTGAATA
TTTAACTACCAAAATAATGGAAGAAGGCATGGGTCAGACTATTTCGTGTC
CTGCTCATGGTTGTGATATCTTAGTGGATGACAACACAGTTATGCGCCTG
ATCACAGATTCAAAAGTTAAATTAAAGTATCAGCATTTAATAACAAATAG
CTTTGTAGAGTGCAATCGACTGTTAAAGTGGTGTCCTGCCCCAGATTGCC
ACCATGTTGTTAAAGTCCAATATCCTGATGCTAAACCTGTTCGCTGCAAA
TGTGGGCGCCAATTTTGCTTTAACTGTGGAGAAAATTGGCATGATCCTGT
TAAATGTAAGTGGTTAAAGAAATGGATTAAAAAGTGTGATGATGACAGTG
AAACCTCCAATTGGATTGCAGCCAACACAAAGGAATGTCCCAAATGCCAT
GTCACAATTGAGAAGGATGGTGGTTGTAATCACATGGTCTGTCGTAACCA
GAATTGTAAAGCAGAGTTTTGCTGGGTGTGTCTTGGCCCATGGGAACCAC
ATGGATCTGCCTGGTACAACTGTAACCGCTATAATGAGGATGATGCAAAG
GCAGCAAGAGATGCACAGGAGCGATCTAGGGCAGCCCTGCAGAGGTACCT
GTTCTACTGTAATCGCTATATGAACCACATGCAGAGCCTGCGCTTTGAGC
ACAAACTATATGCTCAGGTGAAACAGAAAATGGAGGAGATGCAGCAGCAC
AACATGTCCTGGATTGAGGTGCAGTTCCTGAAGAAGGCAGTTGATGTCCT
CTGCCAGTGTCGTGCCACACTCATGTACACTTATGTCTTCGCTTTCTACC
TCAAAAAGAATAACCAGTCCATTATCTTTGAGAATAACCAAGCAGATCTA
GAGAATGCCACAGAGGTGCTCTCGGGCTACCTTGAAAGGGACATCAGTCA
GGACTCTCTGCAGGATATAAAGCAGAAAGTACAAGACAAGTACAGATACT
GTGAGAGTCGACGAAGGGTTTTGTTACAGCATGTGCATGAAGGCTATGAA
AAAGATCTGTGGGAGTACATTGAGGACTGAGCGGCCGC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSMDSDEG
YNYEFDEDEECSEEDSGAEEEEDEDDDEPDDDTLDLGEVELVEPGLGVGG
ERDGLLCGETGGGGGSALGPGGGGGGGGGGGGGGPGHEQEEDYRYEVLTA
EQILQHMVECIREVNEVIQNPATITRILLSHFNWDKEKLMERYFDGNLEK
LFAECHVINPSKKSRTRQMNTRSSAQDMPCQICYLNYPNSYFTGLECGHK
FCMQCWSEYLTTKIMEEGMGQTISCPAHGCDILVDDNTVMRLITDSKVKL
KYQHLITNSFVECNRLLKWCPAPDCHHVVKVQYPDAKPVRCKCGRQFCFN
CGENWHDPVKCKWLKKWIKKCDDDSETSNWIAANTKECPKCHVTIEKDGG
CNHMVCRNQNCKAEFCWVCLGPWEPHGSAWYNCNRYNEDDAKAARDAQER
SRAALQRYLFYCNRYMNHMQSLRFEHKLYAQVKQKMEEMQQHNMSWIEVQ
FLKKAVDVLCQCRATLMYTYVFAFYLKKNNQSIIFENNQADLENATEVLS
GYLERDISQDSLQDIKQKVQDKYRYCESRRRVLLQHVHEGYEKDLWEYIE
D*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU28412
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: siRNA resistant mutant. WT target sequence CGAGATATTTCCCAAGATT changed to AGGGACATCAGTCAGGACT.