cDNA Clone EIF4G1

• Plasmid: pSc-B-EIF4G1 Met1-Glu1242
• Parent Plasmid: pSC-B
• Genbank: NP_886553.3
• Species: Human

Sequence of Insert

ctcgagATGAACAAAGCTCCACAGTCCACAGGCCCCCCACCCGCCCCATC
CCCCGGACTCCCACAGCCAGCGTTTCCCCCGGGGCAGACAGCGCCGGTGG
TGTTCAGTACGCCACAAGCGACACAAATGAACACGCCTTCTCAGCCCCGC
CAGCACTTCTACCCTAGCCGGGCCCAGCCCCCGAGCAGTGCAGCCTCCCG
AGTGCAGAGTGCAGCCCCTGCCCGCCCTGGCCCAGCTGCCCATGTCTACC
CTGCTGGATCCCAAGTAATGATGATCCCTTCCCAGATCTCCTACCCAGCC
TCCCAGGGGGCCTACTACATCCCTGGACAGGGGCGTTCCACATACGTTGT
CCCGACACAGCAGTACCCTGTGCAGCCAGGAGCCCCAGGCTTCTATCCAG
GTGCAAGCCCTACAGAATTTGGGACCTACGCTGGCGCCTACTATCCAGCC
CAAGGGGTGCAGCAGTTTCCCACTGGCGTGGCCCCCGCCCCAGTTTTGAT
GAACCAGCCACCCCAGATTGCTCCCAAGAGGGAGCGTAAGACGATCCGAA
TTCGAGATCCAAACCAAGGAGGAAAGGATATCACAGAGGAGATCATGTCT
GGGGCCCGCACTGCCTCCACACCCACCCCTCCCCAGACGGGAGGCGGTCT
GGAGCCTCAAGCTAATGGGGAGACGCCCCAGGTTGCTGTCATTGTCCGGC
CAGATGACCGGTCACAGGGAGCAATCATTGCTGACCGGCCAGGGCTGCCT
GGCCCAGAGCATAGCCCTTCAGAATCCCAGCCTTCGTCGCCTTCTCCGAC
CCCATCACCATCCCCAGTCTTGGAACCGGGGTCTGAGCCTAATCTCGCAG
TCCTCTCTATTCCTGGGGACACTATGACAACTATACAAATGTCTGTAGAA
GAATCAACCCCCATCTCCCGTGAAACTGGGGAGCCATATCGCCTCTCTCC
AGAACCCACTCCTCTCGCCGAACCCATACTGGAAGTAGAAGTGACACTTA
GCAAACCGGTTCCAGAATCTGAGTTTTCTTCCAGTCCTCTCCAGGCTCCC
ACCCCTTTGGCATCTCACACAGTGGAAATTCATGAGCCTAATGGCATGGT
CCCATCTGAAGATCTGGAACCAGAGGTGGAGTCAAGCCCAGAGCTTGCTC
CTCCCCCAGCTTGCCCCTCCGAATCCCCTGTGCCCATTGCTCCAACTGCC
CAACCTGAGGAACTGCTCAACGGAGCCCCCTCGCCACCAGCTGTGGACTT
AAGCCCAGTCAGTGAGCCAGAGGAGCAGGCCAAGGAGGTGACAGCATCAA
TGGCGCCCCCCACCATCCCCTCTGCTACTCCAGCTACGGCTCCTTCAGCT
ACTTCCCCAGCTCAGGAGGAGGAAATGGAAGAAGAAGAAGAAGAGGAAGA
AGGAGAAGCAGGAGAAGCAGGAGAAGCTGAGAGTGAGAAAGGAGGAGAGG
AACTGCTCCCCCCAGAGAGTACCCCTATTCCAGCCAACTTGTCTCAGAAT
TTGGAGGCAGCAGCAGCCACTCAAGTGGCAGTATCTGTGCCAAAGAGGAG
ACGGAAAATTAAGGAGCTAAATAAGAAGGAGGCTGTTGGAGACCTTCTGG
ATGCCTTCAAGGAGGCGAACCCGGCAGTACCAGAGGTGGAAAATCAGCCT
CCTGCAGGCAGCAATCCAGGCCCAGAGTCTGAGGGCAGTGGTGTGCCCCC
ACGTCCTGAGGAAGCAGATGAGACCTGGGACTCAAAGGAAGACAAAATTC
ACAATGCTGAGAACATCCAGCCCGGGGAACAGAAGTATGAATATAAGTCA
GATCAGTGGAAGCCTCTAAACCTAGAGGAGAAAAAACGTTACGACCGTGA
GTTCCTGCTTGGTTTTCAGTTCATCTTTGCCAGTATGCAGAAGCCAGAGG
GATTGCCACATATCAGTGACGTGGTGCTGGACAAGGCCAATAAAACACCA
CTGCGGCCACTGGATCCCACTAGACTACAAGGCATAAATTGTGGCCCAGA
CTTCACTCCATCCTTTGCCAACCTTGGCCGGACAACCCTTAGCACCCGTG
GGCCCCCAAGGGGTGGGCCAGGTGGGGAGCTGCCCCGTGGGCCGGCTGGC
CTGGGACCCCGGCGCTCTCAGCAGGGACCCCGAAAAGAACCACGCAAGAT
CATTGCCACAGTGTTAATGACCGAAGATATAAAACTGAACAAAGCAGAGA
AAGCCTGGAAACCCAGCAGCAAGCGGACGGCGGCTGATAAGGATCGAGGG
GAAGAAGATGCTGATGGCAGCAAAACCCAGGACCTATTCCGCAGGGTGCG
CTCCATCCTGAATAAACTGACACCCCAGATGTTCCAGCAGCTGATGAAGC
AAGTGACGCAGCTGGCCATCGACACCGAGGAACGCCTCAAAGGGGTCATT
GACCTCATTTTTGAGAAGGCCATTTCAGAGCCCAACTTCTCTGTGGCCTA
TGCCAACATGTGCCGCTGCCTCATGGCGCTGAAAGTGCCCACTACGGAAA
AGCCAACAGTGACTGTGAACTTCCGAAAGCTGTTGTTGAATCGATGTCAG
AAGGAGTTTGAGAAAGACAAAGATGATGATGAGGTTTTTGAGAAGAAGCA
AAAAGAGATGGATGAAGCTGCTACGGCAGAGGAACGAGGACGCCTGAAGG
AAGAGCTGGAAGAGGCTCGGGACATAGCCCGGCGGCGCTCTTTAGGGAAT
ATCAAGTTTATTGGAGAGTTGTTCAAACTGAAGATGTTAACAGAGGCAAT
AATGCATGACTGTGTGGTCAAACTGCTTAAGAACCATGATGAAGAGTCCC
TTGAGTGCCTTTGTCGTCTGCTCACCACCATTGGCAAAGACCTGGACTTT
GAAAAAGCCAAGCCCCGAATGGATCAGTATTTCAACCAGATGGAAAAAAT
CATTAAAGAAAAGAAGACGTCATCCCGCATCCGCTTTATGCTGCAGGACG
TGCTGGATCTGCGAGGGAGCAATTGGGTGCCACGCCGAGGGGATCAGGGT
CCCAAGACCATTGACCAGATCCATAAGGAGGCTGAGATGGAAGAACATCG
AGAGCACATCAAAGTGCAGCAGCTCATGGCCAAGGGCAGTGACAAGCGTC
GGGGCGGTCCTCCAGGCCCTCCCATCAGCCGTGGACTTCCCCTTGTGGAT
GATGGTGGCTGGAACACAGTTCCCATCAGCAAAGGTAGCCGCCCCATTGA
CACCTCACGACTCACCAAGATCACCAAGCCTGGCTCCATCGATTCTAACA
ACCAGCTCTTTGCACCTGGAGGGCGACTGAGCTGGGGCAAGGGCAGCAGC
GGAGGCTCAGGAGCCAAGCCCTCAGACGCAGCATCAGAAGCTGCTCGCCC
AGCTACTAGTACTTTGAATCGCTTCTCAGCCCTTCAACAAGCGGTACCCA
CAGAAAGCACAGATAATAGACGTGTGGTGCAGAGGAGTAGCTTGAGCCGA
GAACGAGGCGAGAAAGCTGGAGACCGAGGAGACCGCCTAGAGCGGAGTGA
ACGGGGAGGGGACCGTGGGGACCGGCTTGATCGTGCGCGGACACCTGCTA
CCAAGCGGAGCTTCAGCAAGGAAGTGGAGGAGCGGAGTAGAGAACGGCCC
TCCCAGCCTGAGGGGCTGCGCAAGGCAGCTAGCCTCACGGAGGATCGGGA
CCGTGGGCGGGATGCCGTGAAGCGAGAAGCTGCCCTACCCCCAGTGAGCC
CCCTGAAGGCGGCTCTCTCTGAGGAGGAGTCTGACCACAACTGAGCGGCC
GC

Amino Acid Sequence

MNKAPQSTGPPPAPSPGLPQPAFPPGQTAPVVFSTPQATQMNTPSQPRQH
FYPSRAQPPSSAASRVQSAAPARPGPAAHVYPAGSQVMMIPSQISYPASQ
GAYYIPGQGRSTYVVPTQQYPVQPGAPGFYPGASPTEFGTYAGAYYPAQG
VQQFPTGVAPAPVLMNQPPQIAPKRERKTIRIRDPNQGGKDITEEIMSGA
RTASTPTPPQTGGGLEPQANGETPQVAVIVRPDDRSQGAIIADRPGLPGP
EHSPSESQPSSPSPTPSPSPVLEPGSEPNLAVLSIPGDTMTTIQMSVEES
TPISRETGEPYRLSPEPTPLAEPILEVEVTLSKPVPESEFSSSPLQAPTP
LASHTVEIHEPNGMVPSEDLEPEVESSPELAPPPACPSESPVPIAPTAQP
EELLNGAPSPPAVDLSPVSEPEEQAKEVTASMAPPTIPSATPATAPSATS
PAQEEEMEEEEEEEEGEAGEAGEAESEKGGEELLPPESTPIPANLSQNLE
AAAATQVAVSVPKRRRKIKELNKKEAVGDLLDAFKEANPAVPEVENQPPA
GSNPGPESEGSGVPPRPEEADETWDSKEDKIHNAENIQPGEQKYEYKSDQ
WKPLNLEEKKRYDREFLLGFQFIFASMQKPEGLPHISDVVLDKANKTPLR
PLDPTRLQGINCGPDFTPSFANLGRTTLSTRGPPRGGPGGELPRGPAGLG
PRRSQQGPRKEPRKIIATVLMTEDIKLNKAEKAWKPSSKRTAADKDRGEE
DADGSKTQDLFRRVRSILNKLTPQMFQQLMKQVTQLAIDTEERLKGVIDL
IFEKAISEPNFSVAYANMCRCLMALKVPTTEKPTVTVNFRKLLLNRCQKE
FEKDKDDDEVFEKKQKEMDEAATAEERGRLKEELEEARDIARRRSLGNIK
FIGELFKLKMLTEAIMHDCVVKLLKNHDEESLECLCRLLTTIGKDLDFEK
AKPRMDQYFNQMEKIIKEKKTSSRIRFMLQDVLDLRGSNWVPRRGDQGPK
TIDQIHKEAEMEEHREHIKVQQLMAKGSDKRRGGPPGPPISRGLPLVDDG
GWNTVPISKGSRPIDTSRLTKITKPGSIDSNNQLFAPGGRLSWGKGSSGG
SGAKPSDAASEAARPATSTLNRFSALQQAVPTESTDNRRVVQRSSLSRER
GEKAGDRGDRLERSERGGDRGDRLDRARTPATKRSFSKEVEERSRERPSQ
PEGLRKAASLTEDRDRGRDAVKREAALPPVSPLKAALSEEESDHN*

• Vector Type: Other
• Antibiotic: Amp; Kan
• DU Number: DU28486
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: delta Gln697 compared to NCBI ref. primer derived extraneous residues (SDHN) between E1242 and 3' Not1 site.