cDNA Clone FancI (Drosophila)

• Synonyms: CG13745; Dmel\CG13745; fanci
• Plasmid: pFBDM 6His GST 3C Drosophila Fancd2/6His 3C Drosophila FancI
• Parent Plasmid: pFBDM
• Expressed: 6His 3C Drosophila FancI
• Genbank: NM_136585.3 GI:665399661 with changes - see notes
• Tag: 6His
• Species: Drosophila Melanogaster

Sequence of Insert

CCCGGGATGCACCACCATCACCATCACTTGGAGGTCTTGTTTCAAGGACCTATGCGGAGTCTGGGTGAGAAGGGGCGCTTTGATGAGCTGGAACTGCTCATTCAAAACACTCCAATAGAAAAGTTAAAGGAAACCCTTAAATCCACAATGACACGCAGCGAGGGCATAACGTATTGGAACTACCTTCTCCGTGGCTTCAATCCGGAGAGCAAGGATGCGCTAGCCAAGCGATTCGAATGCGTCCGCAGCTTCATGGACGGACTGAGCAGCACGGAACTGAGCTACAAACAGACTTTCGATCTGATCACGCGCCTGTCGCAGGACCTGGCCACCTTTCCGTCCGAGCAGCTGGCCTGGATCGTGGAGCACTGCATGGACGGGATCCGACAAGGAGACGCCAAGTGCGTCGGCTGGAAGGATTTGTTGCCGGACGCGCTGTCGCTGCTTTTGGCCAGACCGCGGTTTCTTCTGAACGGCATATCCATAGATGGAATGGAGTTCAGGGACACTACCGTAAGGAGCATTGCCACCATGCAATGGCCAGCCTCAATCCTCACGCCAATTGCCGACATGTTCAAGACCATGAATCTTAGCAGTGGCGAAATTGTGACAGTACTCAACAAATTCTCTGGCGCCCTGCAGGAGCTATCGCCCATGGAACTCCCAGCGCTGTGCTTCCAGCTCTTTTCGATGTGCAGCACTGCTTCCCAGTTGATCATTACGTTGCTGGCCCTGGAGAAGTATTTCCATCGCAACTACTACAAGCGGCTCTTCTCAGACATGTGTAGCAACTCAACGAACTTGGATAGCATTGACTTGTTCTCGGACAAGGAACTAAGGGAGGCCGAAGAGACTATCCTGCATCACTTGAACTACTGTACTATGTACAAACTTACTGAAAAACATTTGGCCATAATGCTGAGAAACTTTCTGCACATGCCCGACGTGATCCTAACTCCGTTTATGCTTAGCGCCATTATCTCGATGACCAGCATTAATCGCGATCCAGAGTCCGCCAGAATATCTCATTCCATTTTGCTACCTTTTTTGCGCAATGTGATCAAAAACAACGAGGAAGAGCGAACGTTAGCCGACTACTCCGTGTGGTATCGGGATACATTGCAACGCAAGCAAGTCGATTTGCAGCAGGTCCTCACGGTGCTCATTGATCAGAACAAAGACGGCAAGGATGTGATAACTCCTGGGCTGGTTCATCTCGTATTTTACTTGCTGAAATCGCAATCGCCCAAGATGCACACATTGGCCATCACATTTCTAACCAAGTTCATTCGCAAGCGATTCATTTTTGGACAAGGCATCATAAAGCTTATTTCAGAGTGGATGATAGTATACCAGGAACAAAATCAGTTCTCGGAATGCCTCACACTTCTAAGTGTGGCTGATACGTATACGGTCAGCGAGTGCATGAAACCAATTCAATCTGTTTTGGAGCACATGCAATGGCTGTCTGGTGAGCAGTCCATGCGCATGATGAACTTTATTCTACCTTTGCTAAAGATCTCTAATCGCGTCCGTGATGCGCTAATTGATGTACTTTGCAAAGCAATGAGCTCGAGTGGAATTCAAAAACGGCGAATGGCGATTTATGGGTTCTGCATGATCCTCAAACAACTAAACAACAGCAATGCTGTGCGCCAGACCTCCAGCGCCACCAGTTTCTGCACTCAACACAGTATCTCTGGCTACTCCATAATGACCCAGAACACACTGGGAAGTCGTAGTAATCCTCAAAGAAATTTCGACATGCTCACGTTGGAAATTATTGGAATGTTGCGAAACTGCTTGCAACAACAATTCGATATACGGTGCTCCCTGTATGAAAATCTTCAGAGAGCAGTGGAGCTCAATGCTAAACTAGTGCCACATGTGCTGCAAGTCATTGACTGGCACTTTCGCAGCTTCTTCGACACCCCCTCTGCAGAGGATGCTGGTGACGATCTAGACACAGTATTCCGCATTCGCTACGATCAATTGGTTAGCTCTAGCGATGATCAACACATGGAGATTCAGCTTAAAGACAACCTGGGCAAGTTGATTCAGTTCGTGGCCAACTGCTTGGCTGTTTTCGATCGAGCTCCATCGGGCTACGACACTCGTGAGATGAATCGGCTCATGAGTTTCTGTGTGGATCGTATGGTGGCAAACAGATTACCCCTGGAAGAGATAACGCCGCCAGCCACACATCTAAAATGTGCCCTCGTTCTGCAGCAACTGAATATCATTGAAGGAATTATTTGCCACTTATTGCTCAAATCCAAGCCACAAAACAATGCTGTTTGTCAAATATTGCCTCTTTTTAATCAGCATTGTAAGCTTTTGGAGAGTCTTAAGGCATTATCGGATGCATCCAAGAAAGCCCATAAACAGTTGAAGCAAACCGCCAAGAATAATAGCACATCAACGTGCAATTTGACACTGAACGGCGTCCCGGTGAAGACCATGTGTACACAGCCTGATAATATTTGGGACTTGGCCATATTGGATAAGCTGCTCCATCTACTTCTTGATGACGTCGTAGCCTTTGCTGCTCCAGAAAAAACAGTGCTCTTGCGCTCCAACGAGCCCCTCGTCCGGTATGTTTTATCCGTAACGGCCAGCAGAGTGGAGTCTATACGCCTGGAGCCCGATTATAAGCAGTTGGCCTACAGTAAACGCACCTTTAAGCAATTGACGGACATTACAAAGGTCATTTACGAGCGCTGCATTCAACGCTTGCCGGAGCTGTGGAAAAATTTCGACATGCAGAGCGCCGCCCTAGCCACTCAGTGTTTTTCTGAATGCCTAAAAACAGCTCATGAAACGTACTCAAAGAAGTTCGAAGATTTTGTCAAGTCATTTGATTTGGCATCTATAAAGGGAAGCAGAAACATGGAAGTGGTTTACACAATTCAGGATGTTTTAGACGACTTTATGACGGAGTACGGCACTGATGATTCGATTTGTAAAAATGAGTTCGTTTCCAAATTGCCTGTTTACCTCTTGGAGTCGCTTGAAATTCTTTTGGATCACATTGACTACCAGGATCGTGCTGCCACCGAATCGTACACTTGGCTGCTAAATTTTTGCAGCAAAAACGAGATTCTCAACAGTGAAATGGGCCTGGTGCACAGAATGCTATTCGTTCAGCGTCAAAAGACCCACTTAGGTCCCTTCTTCGACAGTGTTGCTCGGCAATTGGGTCACGTACTGGGCGTACAAAATGAAGACGCTCCCTCAGAATCTGTGGAGCTAACTCTAAAGTCCATAAGTACAGTGACCATCGAGAGTTGCCTACAGCATCTGTATGCTGCCATCCAAAAGCAGCTGAACGATGTGGACTACTTTGTGACGAAAGCCAACAATCTAAACTACAAATGCCAAGTGGTTCCCGAAATTGACCAGATATATTGGCGGGGCAACCTGGATTCCATGGATCGTTCCATTTGCACACAAATAATCCACATAAGTCGCACTTTGCTGGTGCTCACAAACGTTTGTATTCCTCTAGGTTCTTTGATGGACGGTCTTATGAAGTTGTTGATTCAGCACTACACTTGTTTGAAAAGCCTGACCAAGCACTACATATCGAGCTACACATCAGATACGTCCATTGAGAACATTAAAAGCACCAAATTTGAACTTCTGCTACGTGCAGTTGGTAAACAACTTCCGGCGAATATTTACGAGCTCATCACGTACATAGAAGCTAATACCCTGGATGAGGAAGCGCAGCAGCATACAAAAAAGCGGAAGGTCCAGGCAGAACGTGCTAAAGTTCTGAGAGAGACTCGGCTAATTCCCAAGGTTATAATGGTTATCGAGGACTTCAATAAGCACATTATTCTCCTGTCCAAAAAGACCAAAAGCAAGAATAGACTTACGGACTACCTCCACTTGGGAACCATGCGCGACTTCGATATCAAGTCGACGGATTTGAGGGCAGCTATAGAGCGTAGTATCTCGGATGGCCGCAACATTTCTACCGAAGACAGTAATGCTGAGGATCGGGATGAGGATGAGGATGAAAATGAGAATGTAGAGGAAGACGATGACGCACAGTCCACCACGTATCCAGAGGACTTGGAGGAGAAGGAAGATGAATGCTTAGTCATAGAGAAGAAGGAAGAAAGGCGCAGGAGGCGTGCACCTAGCAGTGAAGCAGAGGATCCACAACCCAAACGAAAAAGAGGCCGCAAAGCTGCAGAACAGCTGGAAGCTGAAGATGAAGAGCAAGTCGAGGAAGAGCAGCCTGAAACGACTTTAAAATTAAGGCGAAACAAAAAAAGTGGAGCCAAAAACCAAAAGGAGTGTGGCCATTAAGGTACC

Amino Acid Sequence

MHHHHHHLEVLFQGPMRSLGEKGRFDELELLIQNTPIEKLKETLKSTMTRSEGITYWNYLLRGFNPESKDALAKRFECVRSFMDGLSSTELSYKQTFDLI
TRLSQDLATFPSEQLAWIVEHCMDGIRQGDAKCVGWKDLLPDALSLLLARPRFLLNGISIDGMEFRDTTVRSIATMQWPASILTPIADMFKTMNLSSGEI
VTVLNKFSGALQELSPMELPALCFQLFSMCSTASQLIITLLALEKYFHRNYYKRLFSDMCSNSTNLDSIDLFSDKELREAEETILHHLNYCTMYKLTEKH
LAIMLRNFLHMPDVILTPFMLSAIISMTSINRDPESARISHSILLPFLRNVIKNNEEERTLADYSVWYRDTLQRKQVDLQQVLTVLIDQNKDGKDVITPG
LVHLVFYLLKSQSPKMHTLAITFLTKFIRKRFIFGQGIIKLISEWMIVYQEQNQFSECLTLLSVADTYTVSECMKPIQSVLEHMQWLSGEQSMRMMNFIL
PLLKISNRVRDALIDVLCKAMSSSGIQKRRMAIYGFCMILKQLNNSNAVRQTSSATSFCTQHSISGYSIMTQNTLGSRSNPQRNFDMLTLEIIGMLRNCL
QQQFDIRCSLYENLQRAVELNAKLVPHVLQVIDWHFRSFFDTPSAEDAGDDLDTVFRIRYDQLVSSSDDQHMEIQLKDNLGKLIQFVANCLAVFDRAPSG
YDTREMNRLMSFCVDRMVANRLPLEEITPPATHLKCALVLQQLNIIEGIICHLLLKSKPQNNAVCQILPLFNQHCKLLESLKALSDASKKAHKQLKQTAK
NNSTSTCNLTLNGVPVKTMCTQPDNIWDLAILDKLLHLLLDDVVAFAAPEKTVLLRSNEPLVRYVLSVTASRVESIRLEPDYKQLAYSKRTFKQLTDITK
VIYERCIQRLPELWKNFDMQSAALATQCFSECLKTAHETYSKKFEDFVKSFDLASIKGSRNMEVVYTIQDVLDDFMTEYGTDDSICKNEFVSKLPVYLLE
SLEILLDHIDYQDRAATESYTWLLNFCSKNEILNSEMGLVHRMLFVQRQKTHLGPFFDSVARQLGHVLGVQNEDAPSESVELTLKSISTVTIESCLQHLY
AAIQKQLNDVDYFVTKANNLNYKCQVVPEIDQIYWRGNLDSMDRSICTQIIHISRTLLVLTNVCIPLGSLMDGLMKLLIQHYTCLKSLTKHYISSYTSDT
SIENIKSTKFELLLRAVGKQLPANIYELITYIEANTLDEEAQQHTKKRKVQAERAKVLRETRLIPKVIMVIEDFNKHIILLSKKTKSKNRLTDYLHLGTM
RDFDIKSTDLRAAIERSISDGRNISTEDSNAEDRDEDEDENENVEEDDDAQSTTYPEDLEEKEDECLVIEKKEERRRRRAPSSEAEDPQPKRKRGRKAAE
QLEAEDEEQVEEEQPETTLKLRRNKKSGAKNQKECGH*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU53448
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: 6His 3C Drosophila FancI XmaI/KpnI into DU53429 (pFBDM 6His GST 3C Drosophila Fancd2).; Various silent changes, plus residue differences:; A8G; P224T; K947M.; Extra residues at C-terminus: SGAKNQKECGH*; (as in DU50185); ; Recommend growth at 30 degrees.