cDNA Clone RBCK1

• Synonyms: C20orf18, HOIL-1, HOIL1, PBMEI, PGBM1, RBCK2, RNF54, UBCE7IP3, XAP3, XAP4, ZRANB4
• Plasmid: pCMV5D RBCK1 Cter C460S IRES GFP donor
• Parent Plasmid: pCMV5D
• Genbank: ENSG00000125826
• Species: Human

Sequence of Insert

gcggccGCCATCCATGAGCAGATGAACTGCAAGGAGTATCAGGAGGACCTGGCCCTGCGGGCTCAGAACGATGTGGCTGCCCGGCAGACGACAGAGATGCTGAAGGTGAGGCTGGGACAGGGCCGAGGCCTAGGGATTTTAAGTTCTGGGATCCAGGTGGGGGCTGGGGGCTTCCCAGTAAGGGCTGTGCTCACACATCCCTGGAGGCTCTGACCTCCCTTCTGGCTGTCACTCCCATCCGGAGGTGGGACTTAGGCCGAATGGTCATGTCAGGAAGAGCGTCTGGGTGGAGGGTGGAGACCACAGGAATGAAGAGGGGGTTGCTGGATGGAGCCTGGCCTGGCAGAGCCACACAGGAGAGACTCCACAGCTCTAGAGGGTCACCACCTTCTCCCTGCCATGGGGAGGGGCCAGGCTGGGTGACTGCCCCAGCCCCGCCCCAGGGCCAGCACCTGCCCCACTCCAGGTGATGCTGCAGCAGGGCGAGGCaATGCGCTGCCCCCAGTGCCAGATCGTGGTACAGAAGAAGGAtGGtaGtGAtTGGATCCGCTGCACCGTCTGCCACACCGAGATtTGCTGGGTCACCAAGGGCCCACGCTGGGGCCCTGGGGTGAGTCTTTGCTCGTGGTGGTGTGGAGAGGGTGCCCTTGTGGGCTTTGCCTTAGAGGAGGGCTGGGAAAACTACAGCCCATGGGCCATATCCAACCCAGCACCTGAATTTGTACAGCTCCCGAGGTAAGAATTTTTTTTTTTTTTTTTGAAACGGAGTTTCACTCTTGTTGCCCAGGCTGGAGTGCAATAGCACAATCTCAGCTCACTGCAACCTCTGCCTTCCGGGTTCAAGCAATTCTCCTGCCTCAGCCTCCCAAGTAGCTGGGATTACAGGCATGCGCCACCACGCCCGGCTAGTTTTGTATTTTTAGGAGAGACGGGGTTTCTCCATGTTGGTCAGACTGGTCTCAAATTCCCGACCGCAGGTGATCCACCTGCCTCGGCCTCCCAAAGTGCTAGGATTACAGGCGTGAGCCACCGGGCCGCAAGAATGTTCTTTACATCTTTAAATGGTTGAAAAAATACCAGAAGATGACTATTTTGTGACGTGAAAATTACATGAAATCCACGTTTCAGTGTCCGTAGGTAAAGTGCTTTGGAGCACAGCCGTGTTCATTCATTCACATGTTGGCTGTGGTTGCTTTTACACTACAGAGGCAGAGGCCGTATGGCTGCCCGGCCCAAAATGACTGGCCCTTTGCAGAAAAGGTTTGCCCACTCCTGCCCTAGAGGACAGCAAGTAAAGGTTCGATCTGGAGTCAGAGGTGGATTGGCCCCTGGCTCTGGCACATACTGACTGTGTGATTAGGCTGCCTGCCACTCACCTCTGTCAGCCCCATgcggccgcTTCCTCTGTGAAATGGAGATGATAGCTGGGCTTATCTCAGGCTTGTTGTCAGAATCAAGTGGAAAAATTGCAAGAGAAGTGCTTAGAGAGCCTGTCACTTGGTGAGCACCCTTGCAGATGGTAGCTGTCGTTATTAGGGGTGTGCTCTGGAATTGGGGGCCTGTTGCTACGTTCAGGTAGACTTTCAGACAGGTGGAGGCAGAGGAAACTGCCCTCTCGCATGCTGACATGTCTAGAATATGCAGAGTGGTCAGATCCTCTCCCTGGCCTGTTCCCGGATCTAGGCGTGGGTAGACTGAGTGCTGTGGGAGCCCAGAAAAGGCCTCAGTGACTCTCCATCAGGTAGCTGAGGCTGACCAGGCCATTCTTGCAGGAGGACCTGCAGAGGCAAAGGCCCGGGGTGGGAGAGCGCTCGGCTGTGGGGGCAGTCTCTGCACTGCGCTGACATTCTCTTCTCTTCCTCCCATCCTCTAGGGCCCAGGAGACACCAGCGGGGGCTGtCGCTGCAGGGTAAATGGGATTCCTTGtCAtCCttctTGTCAGAACTGCCACTGACCCCTCTCCCTCCCCCCCCCCTAACGTTACTGGCCGAAGCCGCTTGGAATAAGGCCGGTGTGCGTTTGTCTATATGTTATTTTCCACCATATTGCCGTCTTTTGGCAATGTGAGGGCCCGGAAACCTGGCCCTGTCTTCTTGACGAGCATTCCTAGGGGTCTTTCCCCTCTCGCCAAAGGAATGCAAGGTCTGTTGAATGTCGTGAAGGAAGCAGTTCCTCTGGAAGCTTCTTGAAGACAAACAACGTCTGTAGCGACCCTTTGCAGGCAGCGGAACCCCCCACCTGGCGACAGGTGCCTCTGCGGCCAAAAGCCACGTGTATAAGATACACCTGCAAAGGCGGCACAACCCCAGTGCCACGTTGTGAGTTGGATAGTTGTGGAAAGAGTCAAATGGCTCTCCTCAAGCGTATTCAACAAGGGGCTGAAGGATGCCCAGAAGGTACCCCATTGTATGGGATCTGATCTGGGGCCTCGGTGCACATGCTTTACATGTGTTTAGTCGAGGTTAAAAAAACGTCTAGGCCCCCCGAACCACGGGGACGTGGTTTTCCTTTGAAAAACACGATGATAATATGGCCACAACCATGGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTGTCCGGCGAGGGCGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCATCTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCCTGACCTACGGCGTGCAGTGCTTCAGCCGCTACCCCGACCACATGAAGCAGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAGCGCACCATCTTCTTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGAAGTTCGAGGGCGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGACTTCAAGGAGGACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCAGAAGAACGGCATCAAGGTGAACTTCAAGATCCGCCACAACATCGAGGACGGCAGCGTGCAGCTCGCCGACCACTACCAGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGACAACCACTACCTGAGCACCCAGTCCGCCCTGAGCAAAGACCCCAACGAGAAGCGCGATCACATGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGATCACTCTCGGCATGGACGAGCTGTACAAGTGAGCTAAAGATGaTcGtGCCACATGCTGACCCAGCCCCACATCCACATTCTGTTAGAATGTAGCTCAGGGAGCTTCGTGGACGGCCTTGCTTGCTGTAGCGTTGTAGGGGCCCTGCCTGCACTGCGGTTGTCCACGGTCACATCTGCCCCAGTGCCTTTGTCCTTCCCTTGGGGCTTGCCGGCCAGACTTCTCTCCCCTGCGGCTCCCACCTCTGCCTGACCCCAGCCTTAAACATAGCCCCTGGCCAGAGGCCTTGCTGGGTGGAGCCTCTGTGTGACTCCATACTCCTCCCACCACAACACTCATCTGTCAAACACCAAGCACTCTCAGCCTCCCCGCCTTCAGCTGTCAGCTTTCTGGGGCTAACTTCTCTGCCTTTGTGGTTGGAGGCCTGAGGCCTCTTGGAACTCTTGCTAACCTGTTCAGAGCCAGGAAGGAGACTGCACAGTTTTGAAAGCACAGCCCGTCAGGTCCGGCTCTGCGTCTCCCTCTCTGCAGCCTGTGTAAGCTATTATAAgctagc

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU54730
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: CRISPR donor designed to introduce a C460S point mutation, use in conjunction with guide pair 54608/54611.