cDNA Clone FAM83H

• Plasmid: pGEX6P-1-FAM83H Q452A
• Parent Plasmid: pGEX6P-1
• Expressed: GST-FAM83H Q452A
• Genbank: NM_198488.3
• Tag: GST
• Species: Human

Sequence of Insert

ggatccATGGCCCGTCGCTCTCAGAGCTCCTCGCAGGGGGACAACCCACT
GGCACCCGGGTACCTGCCGCCTCACTACAAAGAGTACTACCGCCTGGCGG
TGGATGCATTGGCCGAGGGTGGCTCGGAGGCCTACAGCCGCTTCCTCGCT
ACCGAGGGGGCACCAGACTTCCTGTGCCCTGAAGAGCTGGAACATGTGAG
CCGACACCTTCGGCCTCCGCAGTATGTTACCCGAGAGCCACCTGAAGGCA
GCCTTCTCGACGTGGACATGGATGGCTCCTCGGGTACATACTGGCCAGTG
AACTCAGACCAGGCCGTGCCTGAGCTTGATTTGGGCTGGCCTCTGACCTT
CGGCTTCCAGGGCACCGAGGTGACCACCTTGGTGCAGCCACCGCCCCCCG
ACAGCCCCAGTATCAAGGATGAGGCCCGCAGGATGATCCGTTCCGCCCAG
CAGGTGGTGGCCGTGGTGATGGACATGTTCACTGATGTGGACCTGCTCAG
CGAAGTGCTGGAGGCCGCGGCCCGTCGGGTCCCAGTCTACATCCTGCTGG
ATGAGATGAACGCGCAGCACTTCCTGGACATGGCCGACAAGTGCCGTGTC
AACCTGCACCACGTGGATTTCCTGCGCGTACGGACTGTGGCGGGCCCCAC
CTACTACTGCCGCACTGGGAAGTCCTTCAAGGGCCACGTCAAGGAGAAGT
TCCTGCTGGTGGACTGTGCCGTGGTGATGAGTGGGAGCTACAGCTTCATG
TGGTCCTTTGAGAAGATCCACCGCAGCCTGGCGCACGTGTTCCAAGGAGA
GCTGGTCTCCAGCTTCGACGAGGAGTTCCGCATCCTCTTCGCGCAGTCCG
AGCCGCTTGTGCCCTCGGCCGCGGCCCTGGCCCGCATGGACGCCTATGCC
CTGGCTCCGTATGCCGGGGCCGGGCCTCTCGTGGGCGTCCCTGGGGTCGG
GGCGCCAACCCCCTTCTCCTTCCCTAAACGAGCGCACCTCCTGTTCCCGC
CACCCCGGGAAGAGGGCCTGGGCTTCCCCTCCTTCCTCGACCCGGACCGC
CACTTCCTGTCGGCCTTCCGCCGGGAGGAGCCGCCGCGGATGCCGGGGGG
CGCGCTGGAACCGCACGCGGGGCTGCGGCCACTCTCGCGGCGCCTGGAGG
CCGAGGCCGGGCCGGCTGGGGAGCTCGCGGGCGCGCGGGGCTTCTTCCAG
GCGCGGCACCTGGAGATGGACGCCTTCAAGCGGCACAGCTTCGCGACCGA
GGGCGCGGGCGCCGTGGAGAACTTCGCGGCTGCGCGGCAGGTGTCGCGGC
AGACGTTCCTCAGCCACGGCGACGACTTCCGCTTCCAGACCAGCCACTTC
CACCGTGACgcGCTCTACCAGCAGCAGTACCAGTGGGACCCGCAGCTCAC
GCCGGCGCGCCCGCAAGGCCTGTTCGAGAAGCTTCGCGGGGGCCGCGCGG
GTTTCGCGGACCCGGATGACTTCACCCTGGGCGCCGGGCCCCGCTTCCCG
GAGCTCGGACCCGACGGGCACCAGCGGCTGGACTACGTGCCGTCCAGCGC
GTCCCGCGAGGTGCGCCACGGCTCGGACCCCGCCTTCGCGCCCGGACCCC
GCGGCCTGGAGCCCAGCGGAGCCCCGCGCCCCAACCTGACCCAGCGCTTC
CCATGCCAGGCCGCGGCGAGGCCGGGCCCAGACCCCGCTCCCGAGGCGGA
GCCGGAGCGCAGGGGCGGGCCCGAGGGGCGGGCAGGGCTGCGGCGCTGGC
GTTTGGCCTCCTACTTGAGCGGCTGCCACGGCGAGGATGGGGGCGACGAC
GGCCTACCGGCGCCCATGGAAGCGGAGGCTTACGAAGACGACGTGCTGGC
TCCCGGGGGCCGGGCACCTGCCGGCGACCTGCTCCCCTCGGCCTTCCGCG
TCCCAGCAGCCTTCCCCACCAAGGTCCCGGTGCCAGGCCCGGGCAGCGGC
GGCAACGGCCCAGAGCGCGAGGGCCCGGAGGAGCCTGGCCTGGCCAAGCA
GGACTCATTCCGCTCGCGCCTGAACCCCCTGGTCCAGCGCAGCTCCAGGC
TGCGCTCCTCGCTCATCTTCAGCACGTCACAGGCCGAGGGCGCGGCCGGG
GCTGCGGCGGCCACTGAGAAGGTGCAGCTGCTGCACAAGGAGCAGACGGT
CAGCGAGACGCTGGGGCCCGGCGGAGAGGCCGTGCGCTCCGCGGCTTCCA
CCAAGGTGGCGGAGCTGCTGGAGAAGTACAAGGGCCCAGCCCGTGATCCC
GGCGGCGGCGCGGGCGCCATCACCGTTGCCAGCCACAGCAAGGCCGTCGT
GTCCCAGGCGTGGCGGGAAGAGGTGGCGGCCCCAGGTGCCGTGGGGGGCG
AGCGCCGCAGCCTCGAGAGCTGCCTGCTGGACCTGCGCGACTCCTTTGCA
CAGCAGCTGCACCAGGAGGCGGAGCGGCAGCCGGGAGCCGCGTCGCTCAC
CGCGGCGCAGCTGCTCGACACACTGGGCCGGAGCGGCTCCGACCGCCTGC
CTTCCCGCTTCCTCTCTGCCCAGAGCCACTCAACGTCCCCGCAAGGGCTG
GACAGCCCTCTGCCGCTGGAAGGGTCCGGAGCGCACCAGGTGCTCCATAA
TGAGTCAAAAGGGAGCCCCACCTCGGCTTACCCTGAGCGGAAGGGGAGCC
CCACGCCTGGGTTTTCCACTCGAAGAGGAAGTCCAACTACAGGATTTATC
GAGCAGAAGGGGAGCCCCACCTCAGCCTACCCCGAGCGCAGGGGTAGTCC
GGTGCCCCCCGTGCCGGAGCGCAGGAGCAGTCCGGTGCCCCCCGTGCCGG
AGCGCAGGGGCAGCCTCACCCTTACCATCTCCGGGGAGTCCCCGAAGGCC
GGGCCCGCGGAGGAGGGGCCGAGCGGCCCCATGGAAGTCCTGCGCAAAGG
CTCCTTGCGTCTTAGGCAGCTGCTGAGCCCCAAGGGCGAGCGGCGCATGG
AGGATGAGGGTGGCTTCCCAGTGCCGCAGGAGAACGGCCAACCCGAGAGC
CCGCGGCGTCTGTCACTGGGCCAGGGTGACAGCACGGAGGCTGCCACAGA
AGAGCGGGGTCCGCGGGCGCGCCTGTCCTCAGCCACGGCCAACGCCTTGT
ACAGCAGCAACCTTCGGGATGACACGAAGGCCATTCTGGAGCAGATCAGT
GCCCACGGCCAGAAGCACCGTGCGGTCCCTGCCCCGAGCCCCGGCCCGAC
CCACAACAGCCCCGAGCTAGGCCGTCCACCGGCTGCTGGCGTCCTGGCCC
CAGATATGTCCGACAAGGACAAGTGTTCAGCCATCTTCCGCTCGGACAGC
TTGGGGACCCAGGGCCGGCTGAGCCGCACGCTGCCAGCCAGCGCGGAGGA
GCGCGATCGGCTGCTGCGCCGCATGGAGAGCATGCGCAAGGAGAAGCGCG
TGTACAGCCGCTTCGAGGTCTTCTGCAAGAAAGAGGAGGCCAGCAGCCCT
GGGGCAGGGGAAGGCCCCGCGGAGGAGGGCACCAGGGACAGCAAGGTGGG
CAAGTTCGTGCCCAAGATCCTGGGCACGTTCAAAAGCAAGAAGTGAGTCG
AC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMARRSQSSSQGDNPLAPGY
LPPHYKEYYRLAVDALAEGGSEAYSRFLATEGAPDFLCPEELEHVSRHLR
PPQYVTREPPEGSLLDVDMDGSSGTYWPVNSDQAVPELDLGWPLTFGFQG
TEVTTLVQPPPPDSPSIKDEARRMIRSAQQVVAVVMDMFTDVDLLSEVLE
AAARRVPVYILLDEMNAQHFLDMADKCRVNLHHVDFLRVRTVAGPTYYCR
TGKSFKGHVKEKFLLVDCAVVMSGSYSFMWSFEKIHRSLAHVFQGELVSS
FDEEFRILFAQSEPLVPSAAALARMDAYALAPYAGAGPLVGVPGVGAPTP
FSFPKRAHLLFPPPREEGLGFPSFLDPDRHFLSAFRREEPPRMPGGALEP
HAGLRPLSRRLEAEAGPAGELAGARGFFQARHLEMDAFKRHSFATEGAGA
VENFAAARQVSRQTFLSHGDDFRFQTSHFHRDALYQQQYQWDPQLTPARP
QGLFEKLRGGRAGFADPDDFTLGAGPRFPELGPDGHQRLDYVPSSASREV
RHGSDPAFAPGPRGLEPSGAPRPNLTQRFPCQAAARPGPDPAPEAEPERR
GGPEGRAGLRRWRLASYLSGCHGEDGGDDGLPAPMEAEAYEDDVLAPGGR
APAGDLLPSAFRVPAAFPTKVPVPGPGSGGNGPEREGPEEPGLAKQDSFR
SRLNPLVQRSSRLRSSLIFSTSQAEGAAGAAAATEKVQLLHKEQTVSETL
GPGGEAVRSAASTKVAELLEKYKGPARDPGGGAGAITVASHSKAVVSQAW
REEVAAPGAVGGERRSLESCLLDLRDSFAQQLHQEAERQPGAASLTAAQL
LDTLGRSGSDRLPSRFLSAQSHSTSPQGLDSPLPLEGSGAHQVLHNESKG
SPTSAYPERKGSPTPGFSTRRGSPTTGFIEQKGSPTSAYPERRGSPVPPV
PERRSSPVPPVPERRGSLTLTISGESPKAGPAEEGPSGPMEVLRKGSLRL
RQLLSPKGERRMEDEGGFPVPQENGQPESPRRLSLGQGDSTEAATEERGP
RARLSSATANALYSSNLRDDTKAILEQISAHGQKHRAVPAPSPGPTHNSP
ELGRPPAAGVLAPDMSDKDKCSAIFRSDSLGTQGRLSRTLPASAEERDRL
LRRMESMRKEKRVYSRFEVFCKKEEASSPGAGEGPAEEGTRDSKVGKFVP
KILGTFKSKK*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU28561
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Silent changes at Leu35, Pro375 and Ala425 compared to NCBI ref. ; Insert can be excised Bam-Sal or Bam-Not